HUGE

Gene/Protein Characteristic Table for KIAA0736

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00618
Accession No. :	AB018279
Description :	Synaptic vesicle glycoprotein 2A.
HUGO Gene Name :	synaptic vesicle glycoprotein 2A (SV2A)
Clone Name :	hk03846 [Vector Info]
Flexi ORF Clone :	pF1KSDA0736
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 4353 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1690 bp

Genome contig ID gi89161185r_148041558
PolyA signal sequence
(AATAAA,-27)
+----*----+----*----+----*----+----
TGACGAAAAATAAAACGGAAACAAATCCTCAAAAT
Flanking genome sequence
(99942 - 99893)
----+----*----+----*----+----*----+----*----+----*
ACAAAATGGCTATACCTGCTTCTGTGACTCTCCACCTACATCCAATTCTC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 1 r 148141500 148156001 13 99.8 Perfect prediction

Features of the protein sequence	Description

Length: 748 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q7L0J3	100.0	Synaptic vesicl...	Homo sapiens
XP_001166661	99.9	synaptic vesicl...	Pan troglodytes
BAF84407	99.9	unnamed protein...	Homo sapiens
CAD97824	99.7	hypothetical pr...	Homo sapiens
Q5R4L9	99.6	Synaptic vesicl...	Pongo abelii

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018278	3e-140	62.4	KIAA0735
AB028977	1.9e-103	64.2	KIAA1054

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR011701 178 715 PF07690 Major facilitator superfamily MFS_1

HMMTigr IPR005988 7 748 TIGR01299 Synaptic vesicle protein SV2

ProfileScan IPR007114 174 743 PS50850 Major facilitator superfamily

ScanRegExp IPR005829 270 295 PS00217 Sugar transporter superfamily

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR011701	178	715	PF07690	Major facilitator superfamily MFS_1
HMMTigr	IPR005988	7	748	TIGR01299	Synaptic vesicle protein SV2
ProfileScan	IPR007114	174	743	PS50850	Major facilitator superfamily
ScanRegExp	IPR005829	270	295	PS00217	Sugar transporter superfamily

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 174 LYFVLGLALMADGVEVFVVGFVL 196 PRIMARY 23

2 211 GMLGLIVYLGMMVGAFLWGGLAD 233 PRIMARY 23

3 239 QCLLISLSVNSVFAFFSSFVQGY 261 SECONDARY 23

4 266 FCRLLSGVGIGGSIPIVFSYFSE 288 PRIMARY 23

5 301 WLCMFWMIGGVYAAAMAWAIIPH 323 SECONDARY 23

6 337 HSWRVFVLVCAFPSVFAIGALTT 359 PRIMARY 23

7 602 MVYFVSFLGTLAVLPGNIVSALL 624 PRIMARY 23

8 632 RMLAGSSVMSCVSCFFLSF 650 SECONDARY 19

9 659 ALLCLFGGVSIASWNALDVLTVE 681 SECONDARY 23

10 691 AFGFLNALCKLAAVLGISIFTSF 713 SECONDARY 23

11 723 LFASAALALGSSLALKLPETRGQ 745 SECONDARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	174	LYFVLGLALMADGVEVFVVGFVL	196	PRIMARY	23
2	211	GMLGLIVYLGMMVGAFLWGGLAD	233	PRIMARY	23
3	239	QCLLISLSVNSVFAFFSSFVQGY	261	SECONDARY	23
4	266	FCRLLSGVGIGGSIPIVFSYFSE	288	PRIMARY	23
5	301	WLCMFWMIGGVYAAAMAWAIIPH	323	SECONDARY	23
6	337	HSWRVFVLVCAFPSVFAIGALTT	359	PRIMARY	23
7	602	MVYFVSFLGTLAVLPGNIVSALL	624	PRIMARY	23
8	632	RMLAGSSVMSCVSCFFLSF	650	SECONDARY	19
9	659	ALLCLFGGVSIASWNALDVLTVE	681	SECONDARY	23
10	691	AFGFLNALCKLAAVLGISIFTSF	713	SECONDARY	23
11	723	LFASAALALGSSLALKLPETRGQ	745	SECONDARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: ATTCACCTTCGTCATGCTGCG

Primer_r
: ACACTCATACACTGGGCACTG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 1

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: ATTCACCTTCGTCATGCTGCG
Primer_r	: ACACTCATACACTGGGCACTG
PCR product length	: 119 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	1690 bp
Genome contig ID	gi89161185r_148041558
PolyA signal sequence (AATAAA,-27)	+--------+--------+----*----+---- TGACGAAAAATAAAACGGAAACAAATCCTCAAAAT
Flanking genome sequence (99942 - 99893)	----+--------+--------+--------+--------+----* ACAAAATGGCTATACCTGCTTCTGTGACTCTCCACCTACATCCAATTCTC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	1	r	148141500	148156001	13	99.8	Perfect prediction

Gene/Protein Characteristic Table for KIAA0736

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information