HUGE

Gene/Protein Characteristic Table for KIAA0448

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Mapping

Product ID :	ORK00540
Accession No. :	AB007917
Description :	Heparan sulfate 2-O-sulfotransferase 1.
HUGO Gene Name :	heparan sulfate 2-O-sulfotransferase 1 (HS2ST1)
Clone Name :	hg00135 [Vector Info]
Flexi ORF Clone :	pF1KSDA0448
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 6632 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 5279 bp

Genome contig ID gi89161185f_87053026
PolyA signal sequence
(TATAAA,-7)
+----*----+----*----+----*----+----
GGTTTTGTCATTTCATAAATGATATTTTTATAAAT
Flanking genome sequence
(295228 - 295277)
----+----*----+----*----+----*----+----*----+----*
ATTTTTGAATGACTGTACTTTTTACTGATTATCTTAATACCGCCTCACTT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 1 f 87153026 87348252 7 99.2 Perfect prediction

Features of the protein sequence	Description

Length: 362 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q7LGA3	2.8e-157	100.0	Heparan sulfate...	Homo sapiens
XP_001109871	3.3e-157	99.7	heparan sulfate...	Macaca mulatta
Q5R621	5.3e-157	99.7	Heparan sulfate...	Pongo abelii
BAG51177	1.6e-156	99.7	unnamed protein...	Homo sapiens
CAH92556	8e-156	99.4	hypothetical pr...	Pongo abelii

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR007734 15 360 PF05040 Heparan sulphate 2-O-sulphotransferase

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR007734	15	360	PF05040	Heparan sulphate 2-O-sulphotransferase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 16 PKLQLLAVVAFAVAMLFLENQIQ 38 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	16	PKLQLLAVVAFAVAMLFLENQIQ	38	PRIMARY	23

RH mapping information	Description

Chromosome No.

: 1

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: TACATCATGCCCAACCTTTAC
Primer_r	: CATTTATGGCACAGTTTGGAC
PCR product length	: 340 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	5279 bp
Genome contig ID	gi89161185f_87053026
PolyA signal sequence (TATAAA,-7)	+--------+--------+----*----+---- GGTTTTGTCATTTCATAAATGATATTTTTATAAAT
Flanking genome sequence (295228 - 295277)	----+--------+--------+--------+--------+----* ATTTTTGAATGACTGTACTTTTTACTGATTATCTTAATACCGCCTCACTT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	1	f	87153026	87348252	7	99.2	Perfect prediction

Gene/Protein Characteristic Table for KIAA0448

Features of the cloned DNA sequence

Features of the protein sequence

RH mapping information