Gene/Protein Characteristic Table for KIAA1003
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Product ID ORK00164
Accession No AB023220
Description ubiquitin specific peptidase 20, transcript variant 1
Clone name hk09911
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4252 bp)
Predicted protein sequence (917 aa)
Flexi ORF Clone FXC00164
Source Human adult brain
Rouge ID mKIAA1003 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4252 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1378 bp
Genome contig ID gi89161216f_131551910
PolyA signal sequence
(AATAAA,-10)
+----*----+----*----+----*----+----
GTTCAAAATGTCAGAATAAACACAGAATAAATGTT
Flanking genome sequence
(132020 - 132069)
----+----*----+----*----+----*----+----*----+----*
CCCACGGCCACACAGTCGTCTCTCCTTCACTGCAGTTCTGTTACTGGAGG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 9 f 131651909 131683928 25 99.3 Perfect prediction
Features of the protein sequence
Description

Length: 917 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAW87911 0 99.9 ubiquitin speci...
Homo sapiens
AAL79676 0 100.0 pVHL-interactin...
Homo sapiens
AAH39593 0 99.9 Ubiquitin speci...
Homo sapiens
Q9Y2K6 0 99.8 Ubiquitin carbo...
Homo sapiens
XP_520313 0 99.3 ubiquitin speci...
Pan troglodytes
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB029020 3e-125 60.0 KIAA1097
D29956 2.1e-11 29.8 KIAA0055
AB011101 4.7e-10 23.9 KIAA0529
AB020698 1.2e-08 24.5 KIAA0891
AB028986 1.4e-08 31.1 KIAA1063
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR001607 34 110 PF02148 Zinc finger
IPR001394 146 685 PF00443 Peptidase C19
HMMSmart IPR001607 33 85 SM00290 Zinc finger
IPR006615 705 788 SM00695 Ubiquitin carboxyl-terminal hydrolase
IPR006615 813 898 SM00695 Ubiquitin carboxyl-terminal hydrolase
ProfileScan IPR001607 32 96 PS50271 Zinc finger
IPR001394 149 689 PS50235 Peptidase C19
ScanRegExp IPR001394 150 165 PS00972 Peptidase C19
IPR001394 630 647 PS00973 Peptidase C19
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f CCCCACACTACTGCGTCTTTG
Primer_r ACAGAAGATCCGAAAAGTGAG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 9
Experimental conditions
Panel name GeneBridge 4
Primer_f CCCCACACTACTGCGTCTTTG
Primer_r ACAGAAGATCCGAAAAGTGAG
PCR product length 114 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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