Gene/Protein Characteristic Table for KIAA0248
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Product ID ORK00476
Accession No D87435
Description golgi brefeldin A resistant guanine nucleotide exchange factor 1, transcript variant 1
Clone name ha02775s1
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (6360 bp)
Predicted protein sequence (1880 aa)
Flexi ORF Clone FXC00476
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0248 by Kazusa Mouse cDNA Project
Note We replaced ha02775, former representative clones for KIAA0248 with ha02775s1. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 6360 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 556 bp
Genome contig ID gi89161187f_103895315
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
TGGACTTTTTATGATATAATAAATGTCTTAGTACC
Flanking genome sequence
(237326 - 237375)
----+----*----+----*----+----*----+----*----+----*
AGCATCATTGTCTCTCCCTCCGGTTTCCTTCTTGAGCTTGTGGGTCAGGG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 10 f 103995315 104132639 40 99.9 Perfect prediction
Features of the protein sequence
Description

Length: 1880 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_521592 0 99.5 golgi-specific ...
Pan troglodytes
XP_001104407 0 99.0 golgi-specific ...
Macaca mulatta
BAG09674 0 100.0 Golgi-specific ...
synthetic construct
Q92538 0 99.9 Golgi-specific ...
Homo sapiens
AAI17683 0 99.7 GBF1 protein [H...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB029033 5.1e-09 33.0 KIAA1110
AB018306 9.4e-09 37.7 KIAA0763
AB011094 1.5e-08 34.6 KIAA0522
AB023159 2.8e-05 26.1 KIAA0942
AB095931 0.00037 30.9 KIAA2011
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR000904 717 905 PF01369 SEC7-like
HMMSmart IPR000904 717 905 SM00222 SEC7-like
ProfileScan IPR000904 713 903 PS50190 SEC7-like
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 10
Experimental conditions
Panel name Genebridge 4
Primer_f TGTTTCCCACTCCCATTAGCC
Primer_r CCATGGTGCCAGTCAACTCTC
PCR product length 94 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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