##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        Aor51HI     
AscI        AseI        AsnI        Asp718I     AspI        
AvrII       BbrPI       BciVI       BlnI        BsaAI       
BsiWI       BspDI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        EagI        
EclXI       Eco47III    Eco52I      EcoRV       EcoT22I     
FseI        HpaI        KpnI        KspI        MfeI        
MluI        MunI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AflII        4392  CTTAAG               C^TTAAG
AflIII       2845  ACATGT               A^C[GA][CT]GT
Alw44I       1301  GTGCAC               G^TGCAC
ApaLI        1301  GTGCAC               G^TGCAC
AviII        3895  TGCGCA               TGC^GCA
BbeI         4894  GGCGCC               GGCGC^C
BcgI         3749  GCAGACTGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         4392  CTTAAG               C^TTAAG
BglI         4309  GCCGCCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3400  AGATCT               A^GATCT
BsiEI        1478  CGACCG               CG[GA][CT]^CG
BsmBI         131  GAGACG               CGTCTC(1/5)
BspHI        3625  TCATGA               T^CATGA
BspLU11I     2845  ACATGT               A^CATGT
BsrBI        3813  GAGCGG               CCGCTC(-3/-3)
BstEII        808  GGTTACC              G^GT[ACGT]ACC
BstPI         808  GGTTACC              G^GT[ACGT]ACC
DraIII       3475  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I       808  GGTTACC              G^GT[ACGT]ACC
EcoRI        1107  GAATTC               G^AATTC
FspI         3895  TGCGCA               TGC^GCA
HaeII        4894  GGCGCC               [GA]GCGC^[CT]
HindIII      1725  AAGCTT               A^AGCTT
KasI         4894  GGCGCC               G^GCGCC
NarI         4894  GGCGCC               GG^CGCC
NheI         2088  GCTAGC               G^CTAGC
PshAI         944  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         3625  TCATGA               T^CATGA
SfoI         4894  GGCGCC               GGC^GCC
SmaI         1363  CCCGGG               CCC^GGG
SspI          237  AATATT               AAT^ATT
XbaI         1148  TCTAGA               T^CTAGA
XmaCI        1363  CCCGGG               C^CCGGG
XmaI         1363  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          780  GTAGAC               GT^[AC][GT]AC
             4188  GTCTAC              
AccII        1437  CGCG                 CG^CG
             4744  CGCG                
AcyI          133  GACGCC               G[GA]^CG[CT]C
             4894  GGCGCC              
AhdI         4377  GACACTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5205  GACTTACTGTC         
AocI         2883  CCTGAGG              CC^T[ACGT]AGG
             2952  CCTGAGG             
AspEI        4377  GACACTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5205  GACTTACTGTC         
BclI         3580  TGATCA               T^GATCA
             4330  TGATCA              
BsaBI         623  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1712  GATGTACATC          
BsaHI         133  GACGCC               G[GA]^CG[CT]C
             4894  GGCGCC              
BsrDI        5086  CATTGC               GCAATG(2/0)
             3560  GCAATG              
BstUI        1437  CGCG                 CG^CG
             4744  CGCG                
Bsu36I       2883  CCTGAGG              CC^T[ACGT]AGG
             2952  CCTGAGG             
DraI          371  TTTAAA               TTT^AAA
              819  TTTAAA              
DrdI           37  GACTGTGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4179  GACTCAGAGGTC        
Eam1105I     4377  GACACTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5205  GACTTACTGTC         
Eco81I       2883  CCTGAGG              CC^T[ACGT]AGG
             2952  CCTGAGG             
FbaI         3580  TGATCA               T^GATCA
             4330  TGATCA              
HgaI          133  GACGC                GACGC(5/10)
             4465  GCGTC               
Hin1I         133  GACGCC               G[GA]^CG[CT]C
             4894  GGCGCC              
MamI          623  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1712  GATGTACATC          
MvnI         1437  CGCG                 CG^CG
             4744  CGCG                
NaeI         4603  GCCGGC               GCC^GGC
             6097  GCCGGC              
NgoMIV       4603  GCCGGC               G^CCGGC
             6097  GCCGGC              
SexAI         956  ACCAGGT              A^CC[AT]GGT
             3943  ACCAGGT             
SphI         1015  GCATGC               GCATG^C
             1681  GCATGC              
StuI         2150  AGGCCT               AGG^CCT
             2396  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  133 enzymes) #
#########################################

AccIII      AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApaI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BalI        BamHI       
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspEI       BspMI       BsrFI       
BsrGI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MroI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmnI