Gene/Protein Characteristic Table for KIAA0088
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Product ID ORK00404
Accession No D42041
Description glucosidase, alpha; neutral AB, transcript variant 6
Clone name ha01225
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (3820 bp)
Predicted protein sequence (943 aa)
Flexi ORF Clone FXC00404
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0088 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 3820 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 987 bp
Genome contig ID gi51511727r_62048876
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
TCAAAGGAGAAACAATAAAGGGATAAACCATAAAT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AACTGGTGGTAGTCTGGATTCTAGTTCTAGCCTGAGCCACAGACCCTAGA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 11 r 62148876 62170645 24 99.9 Perfect prediction
Features of the protein sequence
Description

Length: 943 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
CAA04006 0 100.0 Glucosidase II ...
Homo sapiens
Q14697 0 99.9 Neutral alpha-g...
Homo sapiens
EAW74050 0 99.7 glucosidase, al...
Homo sapiens
XP_001154003 0 99.5 alpha glucosida...
Pan troglodytes
BAG58692 0 99.7 unnamed protein...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB032987 2.6e-09 26.0 KIAA1161
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR000322 364 809 PF01055 Glycoside hydrolase
ScanRegExp IPR000322 537 544 PS00129 Glycoside hydrolase
IPR000322 644 674 PS00707 Glycoside hydrolase

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 15 ASLVLAFLGVCLGITLAVDRS 35 PRIMARY 21
2 625 LKISIPMCLSLGLVGLSFCGADV 647 PRIMARY 23
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 8
Experimental conditions
Panel name Genebridge 4
Primer_f TTACTCCCCTTTTTATGCCCC
Primer_r ACAAGAAGGGGGCAAAAGAAG
PCR product length 251 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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