Gene/Protein Characteristic Table for KIAA0029
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Product ID ORK00005
Accession No D21852
Description R3H domain containing 1, transcript variant 3
Clone name ha00566
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (4272 bp)
Predicted protein sequence (974 aa)
Flexi ORF Clone FXC00005
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0029 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4272 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 974 bp
Genome contig ID gi89161199f_135905541
PolyA signal sequence
(AATAAA,-17)
+----*----+----*----+----*----+----
CCTTTTTCAGTTCAAGAGAATAAATGTTTACAAAT
Flanking genome sequence
(293767 - 293816)
----+----*----+----*----+----*----+----*----+----*
ATAGGCTCACTTTGTCTTTTTTTTAATTAAAAACACCTTTTAAATGAGTC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 2 f 136005541 136199306 23 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 974 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
BAG11123 0 100.0 R3H domain-cont...
synthetic construct
EAX11627 0 99.9 R3H domain cont...
Homo sapiens
XP_001154157 0 99.5 R3H domain (bin...
Pan troglodytes
XP_856389 0 95.6 similar to R3H ...
Canis lupus fam...
XP_001153781 0 99.5 R3H domain (bin...
Pan troglodytes
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB023219 4e-16 49.8 KIAA1002
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR001374 134 187 PF01424 Single-stranded nucleic acid binding R3H
HMMSmart IPR001374 110 187 SM00393 Single-stranded nucleic acid binding R3H
ProfileScan IPR001374 127 190 PS51061 Single-stranded nucleic acid binding R3H
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 2
Experimental conditions
Panel name Genebridge 4
Primer_f GCCACATTCCCCTCCATTTC
Primer_r AGGGCAGGGGAACCATAAAG
PCR product length 174 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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