Gene/Protein Characteristic Table for KIAA1677
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Product ID ORK05759
Accession No AB051464
Description kelch-like family member 15
Clone name fh18965
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5765 bp)
Predicted protein sequence (521 aa)
Source Human fetal brain
Features of the cloned cDNA sequence
Description

Length: 5765 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 4197 bp
Genome contig ID gi89161218r_23811762
PolyA signal sequence
(AATAAA,-21)
+----*----+----*----+----*----+----
TGAAGAAAAGTTTAAATAAAATATTTTTAATCTTT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAAATATACTTCTTTATCTCTGATTATTTGCTGTTTTTTGATATAATCCA
Features of the protein sequence
Description

Length: 521 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q96M94 0 100.0 Kelch-like prot...
Homo sapiens
BAB71415 0 99.8 unnamed protein...
Homo sapiens
XP_001089787 0 99.8 similar to kelc...
Macaca mulatta
XP_528904 0 99.8 kelch-like 15 [...
Pan troglodytes
CAM26498 0 99.2 kelch-like 15 (...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB037730 1.7e-38 30.6 KIAA1309
AB037775 1.2e-37 30.4 KIAA1354
AB037805 9.4e-28 28.1 KIAA1384
AB018338 2.2e-19 23.7 KIAA0795
D50922 6e-19 25.6 KIAA0132
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR013069 2 45 PF00651 BTB/POZ
IPR011705 50 154 PF07707 BTB/Kelch-associated
IPR006652 233 283 PF01344 Kelch repeat type 1
IPR006652 285 330 PF01344 Kelch repeat type 1
IPR006652 332 392 PF01344 Kelch repeat type 1
IPR006652 394 446 PF01344 Kelch repeat type 1
IPR006652 448 494 PF01344 Kelch repeat type 1
HMMSmart IPR006652 245 296 SM00612 Kelch repeat type 1
IPR006652 297 343 SM00612 Kelch repeat type 1
IPR006652 344 405 SM00612 Kelch repeat type 1
IPR006652 406 459 SM00612 Kelch repeat type 1
IPR006652 460 509 SM00612 Kelch repeat type 1

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 25 AMYVQLIEVVKFCCSFLLAKICL 47 PRIMARY 23
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TCTGTGTCCTCATTACTCTGC
Primer_r CCTTTACTATGACTCGAGCAG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. X
Experimental conditions
Panel name CCR
Primer_f TCTGTGTCCTCATTACTCTGC
Primer_r CCTTTACTATGACTCGAGCAG
PCR product length 137 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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