Gene/Protein Characteristic Table for KIAA1309
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Product ID ORK05758
Accession No AB037730
Description kelch-like family member 13
Clone name fh10381
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5331 bp)
Predicted protein sequence (639 aa)
Source Human fetal brain
Rouge ID mKIAA1309 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5331 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1094 bp
Genome contig ID gi89161218r_116815805
PolyA signal sequence
(AATAAA,-19)
+----*----+----*----+----*----+----
TTATCTTTTTTTGTTCAATAAAAACATTTAATGTC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ATTTTGGTCTGAGACATCCCATTATTGTCTGATTTGACTTCCTTTGATTA
Features of the protein sequence
Description

Length: 639 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
BAH13788 0 100.0 unnamed protein...
Homo sapiens
BAH12978 0 100.0 unnamed protein...
Homo sapiens
BAH12315 0 99.5 unnamed protein...
Homo sapiens
EAW89899 0 99.5 kelch-like 13 (...
Homo sapiens
AAH64576 0 98.4 Kelch-like 13 (...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB037775 0 92.4 KIAA1354
AB037805 5.2e-71 33.8 KIAA1384
AB051474 6.7e-56 30.2 KIAA1687
AB040923 8.5e-55 29.7 KIAA1490
AB032955 4.1e-53 30.6 KIAA1129
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR013069 66 175 PF00651 BTB/POZ
IPR011705 180 281 PF07707 BTB/Kelch-associated
IPR006652 313 360 PF01344 Kelch repeat type 1
IPR006652 362 412 PF01344 Kelch repeat type 1
IPR006652 414 459 PF01344 Kelch repeat type 1
IPR006652 461 506 PF01344 Kelch repeat type 1
IPR006652 508 558 PF01344 Kelch repeat type 1
IPR006652 562 607 PF01344 Kelch repeat type 1
HMMSmart IPR000210 76 175 SM00225 BTB/POZ-like
IPR006652 325 373 SM00612 Kelch repeat type 1
IPR006652 374 425 SM00612 Kelch repeat type 1
IPR006652 426 472 SM00612 Kelch repeat type 1
IPR006652 473 519 SM00612 Kelch repeat type 1
IPR006652 520 571 SM00612 Kelch repeat type 1
IPR006652 572 620 SM00612 Kelch repeat type 1
ProfileScan IPR000210 76 145 PS50097 BTB/POZ-like
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TGACTGTGTGGGTGCATTGAG
Primer_r TAAGTAGAGGGTTCATGCTGC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 9
Experimental conditions
Panel name GeneBridge 4
Primer_f TGACTGTGTGGGTGCATTGAG
Primer_r TAAGTAGAGGGTTCATGCTGC
PCR product length 104 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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