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Gene/Protein Characteristic Table for FLJ00025

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Product ID	ORK05492
Accession No	AK024435
Clone name	as00025
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4559 bp) Predicted protein sequence (955 aa)
Source	Human spleen
Rouge ID	mFLJ00025 by Kazusa Mouse cDNA Project

Length: 4559 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 955 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR001680	43	74	PD000018	G-protein beta WD-40 repeat
HMMPfam	IPR001680	38	75	PF00400	G-protein beta WD-40 repeat
	IPR001680	77	114	PF00400	G-protein beta WD-40 repeat
	IPR001680	118	154	PF00400	G-protein beta WD-40 repeat
	IPR001680	161	202	PF00400	G-protein beta WD-40 repeat
	IPR001680	247	284	PF00400	G-protein beta WD-40 repeat
	IPR001680	440	477	PF00400	G-protein beta WD-40 repeat
HMMSmart	IPR001680	36	75	SM00320	G-protein beta WD-40 repeat
	IPR001680	77	114	SM00320	G-protein beta WD-40 repeat
	IPR001680	116	154	SM00320	G-protein beta WD-40 repeat
	IPR001680	156	202	SM00320	G-protein beta WD-40 repeat
	IPR001680	204	243	SM00320	G-protein beta WD-40 repeat
	IPR001680	245	284	SM00320	G-protein beta WD-40 repeat
	IPR001680	442	477	SM00320	G-protein beta WD-40 repeat
ProfileScan	IPR001680	43	74	PS50082	G-protein beta WD-40 repeat
	IPR001680	43	84	PS50294	G-protein beta WD-40 repeat

RT-PCR-ELISA

Experimental conditions

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