KIAA1761

Product ID	ORK02052
Accession No	AB051548
Description	striatin interacting protein 1, transcript variant 1
Clone name	ha03234
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3237 bp) Predicted protein sequence (837 aa)
HaloTag ORF Clone	FHC02052
Flexi ORF Clone	FXC02052
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA1761 by Kazusa Mouse cDNA Project
Note	We replaced fh26843, former representative clones for KIAA1761 with ha03234. (2001/2/07)

Length: 3237 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	721 bp
Genome contig ID	gi89161185f_110278781
PolyA signal sequence (ATTAAA,-13)	+--------+--------+----*----+---- TGTGATTGTGTATTCTGTTTACATTAAAAAGAAGC
Flanking genome sequence (119999 - 120048)	----+--------+--------+--------+--------+----* AAAAATAATTCCCGTTGGCTTGTCTACAGGAAATATGGCCTCTACGTATC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	f	110378781	110398778	21	99.7	Perfect prediction

Length: 837 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAF83748	99.9	unnamed protein...	Homo sapiens
Q9GLZ5	99.5	Protein FAM40A.	Macaca fascicularis
Q5R7S4	99.3	Protein FAM40A.	Pongo abelii
XP_001493762	98.8	similar to CG11...	Equus caballus
Q0P5J8	98.8	Protein FAM40A.	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB032996	4.9e-78	69.9	KIAA1170

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR012486	50	363	PF07923	N1221-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	512	GLLPSLPQYMIALLKILLAAAP	533	SECONDARY	22
	2	571	KEVIVKAISAVLLLLLKHFKLNH	593	PRIMARY	23
	3	597	FEYMAQHLVFANCIPLILKFFNQ	619	SECONDARY	23

Primer_f	TCCGCCGTGCATCTGAATTTC
Primer_r	GAGCCTCCGCAATTACTTCTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-