##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AclI AflII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BalI BbeI BbrPI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsiEI
BsiWI BspDI BspHI BspLU11I BssHII
BssSI Bst1107I BstBI BstZ17I CelII
ClaI DraI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HpaI KasI MamI MfeI
MluI MluNI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1641 GACGTC GACGT^C
Acc65I 2562 GGTACC G^GTACC
AccI 1782 GTCTAC GT^[AC][GT]AC
AccIII 316 TCCGGA T^CCGGA
AcyI 1641 GACGTC G[GA]^CG[CT]C
AflIII 1426 ACACGT A^C[GA][CT]GT
Alw44I 887 GTGCAC G^TGCAC
ApaLI 887 GTGCAC G^TGCAC
Asp718I 2562 GGTACC G^GTACC
AspI 2204 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 3057 CCTCAGC CCTCAGC(-5/-2)
BcgI 240 GCAGAGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 353 GGATAC GTATCC(6/5)
BsaHI 1641 GACGTC G[GA]^CG[CT]C
BsaI 684 GAGACC GGTCTC(1/5)
BseAI 316 TCCGGA T^CCGGA
Bsp1407I 769 TGTACA T^GTACA
BspEI 316 TCCGGA T^CCGGA
BspMI 1962 GCAGGT ACCTGC(4/8)
BsrGI 769 TGTACA T^GTACA
BstAPI 1925 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 809 GGTGACC G^GT[ACGT]ACC
BstPI 809 GGTGACC G^GT[ACGT]ACC
CpoI 20 CGGTCCG CG^G[AT]CCG
DrdI 645 GACCTCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 809 GGTGACC G^GT[ACGT]ACC
EcoRI 279 GAATTC G^AATTC
HaeII 2344 GGCGCT [GA]GCGC^[CT]
Hin1I 1641 GACGTC G[GA]^CG[CT]C
HindIII 3098 AAGCTT A^AGCTT
KpnI 2562 GGTACC GGTAC^C
MroI 316 TCCGGA T^CCGGA
PflFI 2204 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1164 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 3051 CAGCTG CAG^CTG
RsrII 20 CGGTCCG CG^G[AT]CCG
SacI 243 GAGCTC GAGCT^C
SapI 2499 GAAGAGC GCTCTTC(1/4)
SmaI 99 CCCGGG CCC^GGG
SspBI 769 TGTACA T^GTACA
Tth111I 2204 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 379 CCATGAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 99 CCCGGG C^CCGGG
XmaI 99 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 1012 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
1806 CAGCACCTG
BamHI 319 GGATCC G^GATCC
1112 GGATCC
BanI 104 GGCACC G^G[CT][GA]CC
2562 GGTACC
BsaWI 316 TCCGGA [AT]^CCGG[AT]
367 ACCGGA
BsgI 476 GTGCAG GTGCAG(16/14)
495 GTGCAG
BsmBI 1182 GAGACG CGTCTC(1/5)
2881 GAGACG
BsmI 1828 GCATTC GAATGC(1/-1)
2048 GCATTC
BsrBI 1472 CCGCTC CCGCTC(-3/-3)
1107 GAGCGG
BsrDI 2586 CATTGC GCAATG(2/0)
2263 GCAATG
BsrFI 8 GCCGGC [GA]^CCGG[CT]
77 GCCGGC
BstXI 622 CCATCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2164 CCAAATACTTGG
Cfr10I 8 GCCGGC [GA]^CCGG[CT]
77 GCCGGC
KspI 108 CCGCGG CCGC^GG
118 CCGCGG
NaeI 8 GCCGGC GCC^GGC
77 GCCGGC
NgoMIV 8 GCCGGC G^CCGGC
77 GCCGGC
PpuMI 652 GGGTCCT [GA]G^G[AT]CC[CT]
2696 GGGTCCT
SacII 108 CCGCGG CCGC^GG
118 CCGCGG
SexAI 1810 ACCTGGT A^CC[AT]GGT
2611 ACCAGGT
TfiI 290 GAATC G^A[AT]TC
983 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApaI ApoI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PaeR7I PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoI XhoII