KIAA1169

Product ID	ORK00754
Accession No	AB032995
Description	two pore segment channel 1, transcript variant 2
Clone name	hh10053
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5238 bp) Predicted protein sequence (857 aa)
HaloTag ORF Clone	FHC00754
Flexi ORF Clone	FXC00754
Source	Human adult brain
Rouge ID	mKIAA1169 by Kazusa Mouse cDNA Project
Note	We replaced hg02358, former representative clones for KIAA1169 with hh10053. (2002/5/10)

Length: 5238 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2506 bp
Genome contig ID	gi89161190f_112043751
PolyA signal sequence (ACTAAA,-22)	+--------+--------+----*----+---- TTTCTAGCACATTACTAAAAGAGCCTCTGCTTTGT
Flanking genome sequence (177021 - 177070)	----+--------+--------+--------+--------+----* AAACATGGCTGCTGCCTCTTGACTTCTGTGGCTTTGGGTATTGTCGGGAG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	12	f	112143659	112220770	28	99.5	Perfect prediction

Length: 857 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
NP_001137291	100.0	two pore segmen...	Homo sapiens
EAW98047	100.0	two pore segmen...	Homo sapiens
XP_001111625	98.7	similar to two ...	Macaca mulatta
Q9ULQ1	100.0	Two pore calciu...	Homo sapiens
BAG52729	99.9	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB037777	1.5e-07	25.3	KIAA1356
AB032946	5.6e-07	22.4	KIAA1120
AB032949	1.8e-06	21.9	KIAA1123

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR005821	183	360	PF00520	Ion transport
HMMPfam	IPR005821	518	727	PF00520	Ion transport
ScanRegExp	IPR001220	357	363	PS00307	Legume lectin

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	148	LFYLMELATALLLLLLSLCEAPA	170	PRIMARY	23
	2	176	LGIYVHATLELFALMVVVFELCM	198	PRIMARY	23
	3	215	MVKTSVLVVQFVEAIVVLVRQMS	237	SECONDARY	23
	4	270	LPPFMDILLLLLFFMIIFAILGF	292	PRIMARY	23
	5	303	YFSTLENSIVSLFVLLTTANFPD	325	SECONDARY	23
	6	339	VFFIVYLSIELYFIMNLLLAVVF	361	PRIMARY	23
	7	487	FMYLVVAVNGVWILVETFMLKGG	509	PRIMARY	23
	8	517	PWSYLVFLTIYGVELFLKVAGLG	539	PRIMARY	23
	9	560	FLGLLALALNMEPFYFIVVLRPL	582	PRIMARY	23
	10	612	GLTLLIFYYSFAIVGMEFFCGIV	634	PRIMARY	23
	11	710	MTFYIVTMVVMTIIVAFILEAFV	732	PRIMARY	23

Primer_f	CCAGAGCGAGTTAATGTGTCC
Primer_r	AGCTGTCCCTAACCTCAATGA
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-