Gene/Protein Characteristic Table for KIAA1153
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Product ID ORK00189
Accession No AB032979
Description tRNA methyltransferase 6
Clone name hh01454
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (2944 bp)
Predicted protein sequence (424 aa)
Flexi ORF Clone FXC00189
Source Human adult brain
Rouge ID mKIAA1153 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 2944 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 1669 bp
Genome contig ID gi51511747r_5766510
PolyA signal sequence
(AATAAA,-28)
+----*----+----*----+----*----+----
TGTTCTAAATAAAGTGACTAGAATTTTTGTGTACT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ATTCAATTAAAGGAAACAAATGTCTTTTCCCCCTCTATGGTCTTATGGAT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 20 r 5866510 5879123 9 99.7 Perfect prediction
Features of the protein sequence
Description

Length: 424 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAX10407 1.1e-152 100.0 CGI-09 protein,...
Homo sapiens
Q9UJA5 2e-139 98.2 tRNA (adenine-N...
Homo sapiens
XP_001166710 9.5e-139 97.6 CGI-09 protein ...
Pan troglodytes
XP_001115807 1.5e-137 96.9 similar to CGI-...
Macaca mulatta
XP_542900 1.7e-132 87.4 similar to CGI-...
Canis lupus fam...
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR007316 41 342 PF04189 Eukaryotic initiation factor 3
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f CAATATGGATGTGTGAGTGTG
Primer_r CCCTACTAAACCTTTCTATGC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 20
Experimental conditions
Panel name GeneBridge 4
Primer_f CAATATGGATGTGTGAGTGTG
Primer_r CCCTACTAAACCTTTCTATGC
PCR product length 176 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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