Gene/Protein Characteristic Table for KIAA1027
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Product ID ORK01622
Accession No AB028950
Description talin 1
Clone name fh00156s1
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (8405 bp)
Predicted protein sequence (2550 aa)
Flexi ORF Clone FXC01622
Source Human fetal brain
Rouge ID mKIAA1027 by Kazusa Mouse cDNA Project
Note We replaced fh00156, former representative clones for KIAA1027 with fh00156s1. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 8405 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 450 bp
Genome contig ID gi89161216r_35587338
PolyA signal sequence
(AATAAA,-19)
+----*----+----*----+----*----+----
AAACAGGTCATGCTCCAATAAAAATGATTCTACCT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACAACCTCTGCCTGGCTTCAAGGGAGATACAAGTTTTCTCCCAGGGCAGT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 9 r 35687338 35722367 57 99.9 Perfect prediction
Features of the protein sequence
Description

Length: 2550 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9Y490 0 100.0 Talin-1.
Homo sapiens
AAH42923 0 100.0 Talin 1 [Homo s...
Homo sapiens
AAF27330 0 100.0 talin [Homo sap...
Homo sapiens
EAW58353 0 99.9 talin 1, isofor...
Homo sapiens
AAF23322 0 99.8 talin [Homo sap...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB002318 0 74.3 KIAA0320
AB014555 4.3e-12 22.6 KIAA0655
AB002336 0.0003 20.7 KIAA0338
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR002558 700 771 PD011820 I/LWEQ
IPR002558 1430 1628 PD011820 I/LWEQ
IPR002558 2305 2539 PD011820 I/LWEQ
HMMPfam IPR000299 130 322 PF00373 Band 4.1
IPR015224 500 661 PF09141 Talin
IPR015009 1243 1352 PF08913 Vinculin Binding Site
IPR015009 1858 1982 PF08913 Vinculin Binding Site
IPR002558 2350 2542 PF01608 I/LWEQ
HMMSmart IPR000299 91 322 SM00295 Band 4.1
IPR002558 2345 2542 SM00307 I/LWEQ
ProfileScan IPR000299 95 412 PS50057 Band 4.1
IPR002558 2302 2542 PS50945 I/LWEQ
ScanRegExp IPR000299 182 210 PS00660 Band 4.1
IPR000299 292 321 PS00661 Band 4.1
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TGAAAGAGAAGATGGTTGGCG
Primer_r AAACTTGTACTGCTGCTGCCG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 9
Experimental conditions
Panel name GeneBridge 4
Primer_f TGAAAGAGAAGATGGTTGGCG
Primer_r AAACTTGTACTGCTGCTGCCG
PCR product length 128 (0.3k) bp
PCR conditions 95 °C15 sec64 °C60 sec35 cycles
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