Gene/Protein Characteristic Table for KIAA0848
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Product ID ORK00657
Accession No AB020655
Description SLIT and NTRK-like family, member 3
Clone name hk05607
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4220 bp)
Predicted protein sequence (988 aa)
Flexi ORF Clone FXC00657
Source Human adult brain
Features of the cloned cDNA sequence
Description

Length: 4220 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1175 bp
Genome contig ID gi89161205r_166287204
PolyA signal sequence
(AATAAA,-23)
+----*----+----*----+----*----+----
TATAGCTTTCCTAATAAAAAGTTTTTGAAAAACAT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AATACATTATATATAGAGGATACAAAGCTTGAACCTTAAACTCCAGCTAT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 3 r 166387204 166397163 2 99.0 Perfect prediction
Features of the protein sequence
Description

Length: 988 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_526372 0 99.6 slit and trk li...
Pan troglodytes
BAG09869 0 100.0 SLIT and NTRK-l...
synthetic construct
O94933 0 99.9 SLIT and NTRK-l...
Homo sapiens
XP_851171 0 98.3 similar to slit...
Canis lupus fam...
XP_001504616 0 97.4 SLIT and NTRK-l...
Equus caballus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB020725 1.6e-78 42.2 KIAA0918
AB058757 1.8e-34 50.3 KIAA1854
AB067497 1.2e-27 38.9 KIAA1910
AB011538 5.7e-09 25.3 KIAA0814
AB007865 8.1e-07 27.9 KIAA0405
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR001611 209 222 PR00019 Leucine-rich repeat
IPR001611 466 479 PR00019 Leucine-rich repeat
HMMPfam IPR001611 113 135 PF00560 Leucine-rich repeat
IPR001611 161 183 PF00560 Leucine-rich repeat
IPR001611 185 207 PF00560 Leucine-rich repeat
IPR001611 208 231 PF00560 Leucine-rich repeat
IPR001611 420 442 PF00560 Leucine-rich repeat
IPR001611 444 466 PF00560 Leucine-rich repeat
IPR001611 468 490 PF00560 Leucine-rich repeat
IPR001611 492 514 PF00560 Leucine-rich repeat
IPR001611 516 538 PF00560 Leucine-rich repeat
HMMSmart IPR003591 111 134 SM00369 Leucine-rich repeat
IPR003591 135 158 SM00369 Leucine-rich repeat
IPR003591 159 182 SM00369 Leucine-rich repeat
IPR003591 183 206 SM00369 Leucine-rich repeat
IPR003591 207 230 SM00369 Leucine-rich repeat
IPR000483 243 293 SM00082 Cysteine-rich flanking region
IPR003591 442 465 SM00369 Leucine-rich repeat
IPR003591 466 489 SM00369 Leucine-rich repeat
IPR003591 490 513 SM00369 Leucine-rich repeat
IPR003591 514 537 SM00369 Leucine-rich repeat
IPR000483 573 623 SM00082 Cysteine-rich flanking region

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 24 RMLWIILLSTIALGWTTPIPLIE 46 PRIMARY 23
2 665 SVLILSLLVLFFSAVFVAAGLFA 687 PRIMARY 23
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f CTTGCTCCCTTTACACTTGTC
Primer_r TTAATGATCTGAGTATGGCCC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 3
Experimental conditions
Panel name GeneBridge 4
Primer_f CTTGCTCCCTTTACACTTGTC
Primer_r TTAATGATCTGAGTATGGCCC
PCR product length 151 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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