Gene/Protein Characteristic Table for KIAA0827
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Product ID ORK01608
Accession No AB020634
Description nuclear factor of activated T-cells 5, tonicity-responsive, transcript variant 3
Clone name hh03726
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (6019 bp)
Predicted protein sequence (1608 aa)
Flexi ORF Clone FXC01608
Source Human adult brain
Rouge ID mKIAA0827 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 6019 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1105 bp
Genome contig ID gi51511732f_68057388
PolyA signal sequence
(TATAAA,-10)
+----*----+----*----+----*----+----
AATTTTTCCCTCTAATAGGAAACAGTATAAATTTT
Flanking genome sequence
(231474 - 231523)
----+----*----+----*----+----*----+----*----+----*
AATTAAAAAAAAAAGGCAAACTAAAATTTCTTGAAATATCACTTCTCCCT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 16 f 68157388 68288860 14 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 1608 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_001093880 0 99.1 similar to nucl...
Macaca mulatta
O94916 0 100.0 Nuclear factor ...
Homo sapiens
XP_001168930 0 97.1 nuclear factor ...
Pan troglodytes
NP_619727 0 98.8 nuclear factor ...
Homo sapiens
AAI31510 0 98.8 NFAT5 protein [...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB028971 0.00056 26.9 KIAA1048
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR008366 384 400 PR01789 Nuclear factor of activated T cells (NFAT)
IPR008366 502 524 PR01789 Nuclear factor of activated T cells (NFAT)
IPR008366 543 562 PR01789 Nuclear factor of activated T cells (NFAT)
IPR008366 601 620 PR01789 Nuclear factor of activated T cells (NFAT)
HMMPfam IPR011539 359 516 PF00554 Rel homology
IPR002909 522 618 PF01833 Cell surface receptor IPT/TIG
HMMSmart IPR002909 521 619 SM00429 Cell surface receptor IPT/TIG
ProfileScan IPR011539 341 520 PS50254 Rel homology
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TTGGAGAAACTGAAGGGTAAC
Primer_r GAAACCCAGAGAACTAACAAC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 16
Experimental conditions
Panel name GeneBridge 4
Primer_f TTGGAGAAACTGAAGGGTAAC
Primer_r GAAACCCAGAGAACTAACAAC
PCR product length 161 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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