Gene/Protein Characteristic Table for KIAA0741
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Product ID ORK00619
Accession No AB018284
Description eukaryotic translation initiation factor 5B
Clone name hk04024
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4177 bp)
Predicted protein sequence (1222 aa)
Flexi ORF Clone FXC00619
Source Human adult brain
Rouge ID mKIAA0741 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4177 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 364 bp
Genome contig ID gi89161199f_99220300
PolyA signal sequence
(ATTAAA,-31)
+----*----+----*----+----*----+----
ACTGATTAAATCAGTACTGCAGTATTTGATTAACC
Flanking genome sequence
(162375 - 162424)
----+----*----+----*----+----*----+----*----+----*
AAGCTTCTGCAGATTTTGTGATTCTTGGGACTTTTTTGACGTAAGAAATA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 2 f 99320300 99382673 24 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 1222 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
BAG09828 0 100.0 eukaryotic tran...
synthetic construct
EAX01867 0 99.9 eukaryotic tran...
Homo sapiens
O60841 0 99.8 Eukaryotic tran...
Homo sapiens
AAH32639 0 99.8 Eukaryotic tran...
Homo sapiens
CAB44357 0 99.7 IF2 protein [Ho...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR000178 823 906 PD186100 Initiation factor 2
FPrintScan IPR000795 635 648 PR00315 Protein synthesis factor
IPR000795 701 711 PR00315 Protein synthesis factor
IPR000795 717 728 PR00315 Protein synthesis factor
IPR000795 753 762 PR00315 Protein synthesis factor
HMMPfam IPR000795 631 848 PF00009 Protein synthesis factor
IPR004161 872 950 PF03144 Translation elongation factor EFTu/EF1A
HMMTigr IPR005225 631 806 TIGR00231 Small GTP-binding protein domain
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f GTGACTGGCAGCTTATTGTGG
Primer_r CCATCAGTGTCCAAATACGTC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 2
Experimental conditions
Panel name UniGene
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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