Gene/Protein Characteristic Table for KIAA0698
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Product ID ORK05414
Accession No AB014598
Description hephaestin, transcript variant 2
Clone name hg00360s1
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4228 bp)
Predicted protein sequence (1104 aa)
Flexi ORF Clone FXC05414
Source Human adult brain
Rouge ID mKIAA0698 by Kazusa Mouse cDNA Project
Note We replaced hg00360, former representative clones for KIAA0698 with hg00360s1. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 4228 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 714 bp
Genome contig ID gi89161218f_65200834
PolyA signal sequence
(ATTAAA,-24)
+----*----+----*----+----*----+----
AAACCCTATCCATTAAAGTACTTGTTAGAACACTG
Flanking genome sequence
(203121 - 203170)
----+----*----+----*----+----*----+----*----+----*
AAAGACTGAATTTGGTGATTTTTGACAGTCTCTGTGGCTTCTGTCACTGT
Features of the protein sequence
Description

Length: 1104 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9BQS7 0 100.0 Hephaestin; Fla...
Homo sapiens
EAX05385 0 100.0 hephaestin, iso...
Homo sapiens
CAC35365 0 99.9 hephaestin [Hom...
Homo sapiens
AAQ89349 0 99.9 HEPH [Homo sapi...
Homo sapiens
XP_001099464 0 96.9 similar to heph...
Macaca mulatta
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR001117 176 313 PF00394 Multicopper oxidase
IPR011707 401 428 PF07732 Multicopper oxidase
IPR011706 896 1012 PF07731 Multicopper oxidase
ScanRegExp IPR002355 287 307 PS00079 Multicopper oxidase
IPR002355 639 659 PS00079 Multicopper oxidase
IPR002355 986 1006 PS00079 Multicopper oxidase
IPR002355 991 1002 PS00080 Multicopper oxidase

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 1054 LASVLVAISVTLLLVVLALGGVV 1076 PRIMARY 23
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f CTCAACATTCCTTTCAGTGCC
Primer_r TGAAGGTAGGCTGAGTATTGG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. X
Experimental conditions
Panel name GeneBridge 4
Primer_f CTCAACATTCCTTTCAGTGCC
Primer_r TGAAGGTAGGCTGAGTATTGG
PCR product length 183 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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