Gene/Protein Characteristic Table for KIAA0448
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Product ID ORK00540
Accession No AB007917
Description heparan sulfate 2-O-sulfotransferase 1, transcript variant 1
Clone name hg00135
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (6632 bp)
Predicted protein sequence (362 aa)
Flexi ORF Clone FXC00540
Source Human adult brain
Rouge ID mKIAA0448 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 6632 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 5279 bp
Genome contig ID gi89161185f_87053026
PolyA signal sequence
(TATAAA,-7)
+----*----+----*----+----*----+----
GGTTTTGTCATTTCATAAATGATATTTTTATAAAT
Flanking genome sequence
(295228 - 295277)
----+----*----+----*----+----*----+----*----+----*
ATTTTTGAATGACTGTACTTTTTACTGATTATCTTAATACCGCCTCACTT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 1 f 87153026 87348252 7 99.2 Perfect prediction
Features of the protein sequence
Description

Length: 362 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q7LGA3 2.8e-157 100.0 Heparan sulfate...
Homo sapiens
XP_001109871 3.3e-157 99.7 heparan sulfate...
Macaca mulatta
Q5R621 5.3e-157 99.7 Heparan sulfate...
Pongo abelii
BAG51177 1.6e-156 99.7 unnamed protein...
Homo sapiens
CAH92556 8e-156 99.4 hypothetical pr...
Pongo abelii
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR007734 15 360 PF05040 Heparan sulphate 2-O-sulphotransferase

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 16 PKLQLLAVVAFAVAMLFLENQIQ 38 PRIMARY 23
Experimental conditions
Primer_f
Primer_r
PCR conditions °C sec °C sec cycles

RH mapping information
Description

Chromosome No. 1
Experimental conditions
Panel name GeneBridge 4
Primer_f TACATCATGCCCAACCTTTAC
Primer_r CATTTATGGCACAGTTTGGAC
PCR product length 340 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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