##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
ApaI ApaLI AscI Asp718I AviII
AvrII BbeI BbrPI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaWI
BseAI BsgI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BssHII
BssSI BstBI BstEII BstPI CelII
Cfr10I ClaI CpoI DraIII EcoO65I
FseI FspI HpaI KasI KpnI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PvuI RsrII SacI
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AclI 1563 AACGTT AA^CGTT
AcyI 211 GGCGTC G[GA]^CG[CT]C
AflIII 705 ACACGT A^C[GA][CT]GT
AocI 291 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2738 AGCGCT AGC^GCT
AspI 1392 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 266 CCCGAG C^[CT]CG[GA]G
BamHI 774 GGATCC G^GATCC
BanI 4724 GGTGCC G^G[CT][GA]CC
BbsI 1398 GTCTTC GAAGAC(2/6)
BbvCI 70 GCTGAGG CCTCAGC(-5/-2)
BcgI 13 GCAGCCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4032 GTATCC GTATCC(6/5)
BpuAI 1398 GTCTTC GAAGAC(2/6)
BsaBI 2873 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 211 GGCGTC G[GA]^CG[CT]C
BsiEI 56 CGGCCG CG[GA][CT]^CG
BsmI 961 GAATGC GAATGC(1/-1)
BsoBI 266 CCCGAG C^[CT]CG[GA]G
Bst1107I 3396 GTATAC GTA^TAC
BstAPI 3493 GCACCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3778 CCATTGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3396 GTATAC GTA^TAC
Bsu36I 291 CCTCAGG CC^T[ACGT]AGG
DrdI 1484 GACAGTAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 56 CGGCCG C^GGCCG
EclXI 56 CGGCCG C^GGCCG
Eco47III 2738 AGCGCT AGC^GCT
Eco52I 56 CGGCCG C^GGCCG
Eco81I 291 CCTCAGG CC^T[ACGT]AGG
EcoRI 4575 GAATTC G^AATTC
EcoRV 2157 GATATC GAT^ATC
HgaI 212 GCGTC GACGC(5/10)
Hin1I 211 GGCGTC G[GA]^CG[CT]C
KspI 249 CCGCGG CCGC^GG
MamI 2873 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 4535 CATATG CA^TATG
PflFI 1392 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3009 GACTAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1563 AACGTT AA^CGTT
PstI 6586 CTGCAG CTGCA^G
SacII 249 CCGCGG CCGC^GG
SapI 354 GCTCTTC GCTCTTC(1/4)
SphI 5950 GCATGC GCATG^C
Tth111I 1392 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3831 TCTAGA T^CTAGA
XcmI 913 CCAGAGAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 4499 CTTAAG C^TTAAG
4996 CTTAAG
AhdI 422 GACACGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2540 GACAAGGAGTC
AspEI 422 GACACGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2540 GACAAGGAGTC
AspHI 908 GTGCTC G[AT]GC[AT]^C
3703 GAGCAC
BalI 948 TGGCCA TGG^CCA
6506 TGGCCA
BclI 506 TGATCA T^GATCA
5305 TGATCA
BfrI 4499 CTTAAG C^TTAAG
4996 CTTAAG
BsaI 1292 GAGACC GGTCTC(1/5)
3633 GGTCTC
BsiHKAI 908 GTGCTC G[AT]GC[AT]^C
3703 GAGCAC
BsmBI 213 CGTCTC CGTCTC(1/5)
846 GAGACG
BsrBI 235 CCGCTC CCGCTC(-3/-3)
393 CCGCTC
DraII 230 GGGTCCC [GA]G^G[ACGT]CC[CT]
2566 AGGTCCT
Eam1105I 422 GACACGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2540 GACAAGGAGTC
EcoNI 109 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5033 CCTTTAAGAGG
EcoO109I 230 GGGTCCC [GA]G^G[ACGT]CC[CT]
2566 AGGTCCT
EcoT14I 2127 CCATGG C^C[AT][AT]GG
3764 CCATGG
EcoT22I 4538 ATGCAT ATGCA^T
4882 ATGCAT
FbaI 506 TGATCA T^GATCA
5305 TGATCA
HaeII 105 AGCGCC [GA]GCGC^[CT]
2738 AGCGCT
HindIII 2470 AAGCTT A^AGCTT
2947 AAGCTT
MluNI 948 TGGCCA TGG^CCA
6506 TGGCCA
MscI 948 TGGCCA TGG^CCA
6506 TGGCCA
NcoI 2127 CCATGG C^CATGG
3764 CCATGG
NsiI 4538 ATGCAT ATGCA^T
4882 ATGCAT
PflMI 375 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1420 CCACAGTCTGG
PpuMI 230 GGGTCCC [GA]G^G[AT]CC[CT]
2566 AGGTCCT
StyI 2127 CCATGG C^C[AT][AT]GG
3764 CCATGG
SwaI 1952 ATTTAAAT ATTT^AAAT
4901 ATTTAAAT
Van91I 375 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1420 CCACAGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PshBI
PvuII RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI