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Gene/Protein Characteristic Table for KIAA0380

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Product ID	ORK00522
Accession No	AB002378
Description	Rho guanine nucleotide exchange factor (GEF) 11, transcript variant 1
Clone name	hh00518
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector. This cDNA clone hh00518 has an irregular adapter sequence (GTCGACCCAC GCGTCCGCCC ACGCGTCCG) instead of regular one (GTCGACCCAC GCGTCCG).
cDNA sequence	DNA sequence (5790 bp) Predicted protein sequence (1539 aa)
HaloTag ORF Clone	FHC00522
Flexi ORF Clone	FXC00522
Source	Human adult brain
Rouge ID	mKIAA0380 by Kazusa Mouse cDNA Project

Length: 5790 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 1539 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001478	64	137	PF00595	PDZ/DHR/GLGF
	IPR015212	324	503	PF09128	Regulator of G protein signalling-like domain
	IPR000219	755	939	PF00621	DH
HMMSmart	IPR001478	72	140	SM00228	PDZ/DHR/GLGF
	IPR000342	330	449	SM00315	Regulator of G protein signalling
	IPR000219	755	939	SM00325	DH
	IPR001849	983	1098	SM00233	Pleckstrin-like
ProfileScan	IPR001478	64	128	PS50106	PDZ/DHR/GLGF
	IPR000342	330	436	PS50132	Regulator of G protein signalling
	IPR000219	751	940	PS50010	DH
	IPR001849	982	1096	PS50003	Pleckstrin-like

RT-PCR

Experimental conditions

Chromosome No. 1
Experimental conditions

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