Gene/Protein Characteristic Table for KIAA0189
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Product ID ORK01660
Accession No D80011
Description StAR-related lipid transfer (START) domain containing 8, transcript variant 2
Clone name ha02928
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (4824 bp)
Predicted protein sequence (1132 aa)
Flexi ORF Clone FXC01660
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0189 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4824 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning Warning
Integrity of 3' end
Length of 3'UTR 1415 bp
Genome contig ID gi89161218f_67730218
PolyA signal sequence
(AATAAA,-20)
+----*----+----*----+----*----+----
AGGATCCCTCAAATCAATAAAGCATTACCAGAGAT
Flanking genome sequence
(132184 - 132233)
----+----*----+----*----+----*----+----*----+----*
GCACTTTAACTGTGTGGCTGTCTTTGCTGACAATGGGCTAGGGACTGGGG
Features of the protein sequence
Description

Length: 1132 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
NP_001135975 0 99.9 StAR-related li...
Homo sapiens
CAH18253 0 99.8 hypothetical pr...
Homo sapiens
XP_001082337 0 97.0 similar to STAR...
Macaca mulatta
BAG10301 0 100.0 START domain-co...
synthetic construct
Q92502 0 99.9 StAR-related li...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB051510 8e-85 42.6 KIAA1723
D50921 2.1e-05 31.6 KIAA0131
AB051509 2.5e-05 23.8 KIAA1722
D87717 0.0001 31.3 KIAA0013
AB037735 0.00024 25.7 KIAA1314
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR000198 695 844 PF00620 RhoGAP
IPR002913 927 1129 PF01852 Lipid-binding START
HMMSmart IPR000198 692 883 SM00324 RhoGAP
IPR002913 927 1129 SM00234 Lipid-binding START
ProfileScan IPR000198 682 886 PS50238 RhoGAP
IPR002913 931 1101 PS50848 Lipid-binding START
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. X
Experimental conditions
Panel name Genebridge 4
Primer_f TAACATAACAGCCCCTTTCTC
Primer_r AGTGGGACAGAACAGGTTTGG
PCR product length 107 bp
PCR conditions 95 °C15 sec60 °C60 sec30 cycles
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