Gene/Protein Characteristic Table for KIAA0071
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Product ID ORK06618
Accession No D31888
Description REST corepressor 1
Clone name ha00472
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (5241 bp)
Predicted protein sequence (396 aa)
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0071 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5241 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 4050 bp
Genome contig ID gi51511730f_102029355
PolyA signal sequence
(AATAAA,-26)
+----*----+----*----+----*----+----
GAGGGGTTAAATAAAAATTTTCAAGCCATTGATGT
Flanking genome sequence
(237291 - 237340)
----+----*----+----*----+----*----+----*----+----*
AATAAAATATGAAATGAAAGCATTTTCTGCACCCCCACGCTCCATCAAAG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 14 f 102129244 102266644 12 99.2 Perfect prediction
Features of the protein sequence
Description

Length: 396 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9UKL0 2.9e-150 100.0 REST corepresso...
Homo sapiens
EAW81793 2.9e-150 100.0 REST corepresso...
Homo sapiens
EAW81794 7.7e-146 100.0 REST corepresso...
Homo sapiens
Q8CFE3 2.3e-145 96.2 REST corepresso...
Mus musculus
XP_001927812 7.1e-145 95.5 similar to REST...
Sus scrofa
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB037764 2.8e-40 64.4 KIAA1343
AB028970 1.6e-10 25.8 KIAA1047
AB033092 0.00054 26.6 KIAA1266
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR000949 14 78 PF01448 ELM2
IPR014778 103 148 PF00249 Myb
IPR014778 294 339 PF00249 Myb
HMMSmart IPR001005 102 150 SM00717 SANT
IPR001005 293 341 SM00717 SANT
ProfileScan IPR000949 14 100 PS51156 ELM2
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 14
Experimental conditions
Panel name Stanford G3
Primer_f GTTTCCTCAGATACCACAGAC
Primer_r GAGCATCGCACAGGACCACTA
PCR product length 337 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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