Strain-specific differences in gene expression have been observed among
various inbred mouse strains. Two strains that are commonly used
in gene-targeting research today are the 129 substrains, which are used
to produce ES cell lines, and C57BL/6J, which is used for the extensive
backcrosses required to produce isogenic knockout mice. When F2 nonisogenic
littermates are assessed using DNA microarrays, one must determine whether
the expression profiles obtained resulted either from specific alteration(s)
induced by the targeted gene mutation or from gene expression differences
related to the genetic background of the parent mouse strains. In the present
study, we report the differential expression profile of genes expressed
in neonatal brains and adult spleen and liver of 129X1/SvJ and C57BL/6J
strains of mice. These comprehensive profiles were assessed using two types
of Agilent Mouse Oligo Microarrays (development and standard) and were
compiled into a publicly available database. Researchers can use this database
to determine whether their microarray findings represent strain-specific
differences in gene expression by comparing their data with those cataloged
in our database. This database is useful for effectively analyzing DNA
microarray data from nonisogenic littermates, and would help researchers
avoid time-consuming backcrosses and confirmative experiments requiring
the use of many mice.
| T1_description | T1_excel file (.xls) | T1_original data |
| T2_description | T2_excel file (.xls) | T2_original data |
| T3_description | T3_excel file (.xls) | T3_original data |
| T4_description | T4_excel file (.xls) | T4_original data |
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How to obtain KIAA
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