PDE7B ORF clone pFN21ASDB0003

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21ASDB0003

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Clone info.

Species

human

Clone Name

pFN21ASDB0003 Sequence (with vector) Restriction Map

Product ID

FHC00973

Flexi Clone PID

FXC00973

Original Clone PID

ORK00973

ORF Sequence

DNA sequence (1506 bp)
Protein sequence (502 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384352]

Gene

KIBB0003

Symbol

PDE7B

Alias : bA472E5.1

Description

phosphodiesterase 7B

Original Clone ID

ah00907

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1506 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 502 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5175.1 2.6e-181 100.0 PDE7B CCDS56538.1 8.6e-111 57.8 PDE7A CCDS34901.1 9.6e-111 60.8 PDE7A CCDS54858.1 2.5e-44 30.4 PDE4D CCDS56371.1 2.5e-44 30.4 PDE4D The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR023088 220 233 PR00387 3'5'-cyclic nucleotide phosphodiesterase IPR023088 251 264 PR00387 3'5'-cyclic nucleotide phosphodiesterase IPR023088 265 280 PR00387 3'5'-cyclic nucleotide phosphodiesterase IPR023088 292 308 PR00387 3'5'-cyclic nucleotide phosphodiesterase IPR023088 371 384 PR00387 3'5'-cyclic nucleotide phosphodiesterase IPR023088 388 404 PR00387 3'5'-cyclic nucleotide phosphodiesterase HMMPfam IPR002073 224 448 PF00233 3'5'-cyclic nucleotide phosphodiesterase HMMSmart IPR003607 222 389 SM00471 Metal-dependent phosphohydrolase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_005266988 502 604645 100.0 100.0 NP_061818 450 604645 100.0 84.3 XP_011534057 489 604645 100.0 84.3 XP_016869027 448 171885 59.7 85.5 NP_001229247 482 171885 57.8 89.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDB0003 Download

>KIBB0003  1506 bp
ATGCCTGTGCTAGAGCGATATTTCCACCCAGCAGAGCTAGGCAGGAGGTGGACAGGCCCA
GAAGGTGTGCTGCCCTCCTCCCCGGGAAGCCGGCCGGGGTGCCAGCAGGGGCCGCTGCCC
TGGGACTTGCCAGAGATGATCAGGATGGTAAAGCTGGTTTGGAAATCCAAAAGTGAGCTG
CAGGCGACCAAACAGAGAGGCATTCTGGACAATGAAGATGCTCTCCGCAGCTTTCCAGGA
GATATACGACTAAGGGGTCAGACGGGGGTTCGTGCTGAACGCCGTGGCTCCTACCCATTC
ATTGACTTCCGCCTACTTAACAGTACAACATACTCAGGGGAGATTGGCACCAAGAAAAAG
GTGAAAAGACTATTAAGCTTTCAAAGATACTTCCATGCATCAAGGCTGCTTCGTGGAATT
ATACCACAAGCCCCTCTGCACCTGCTGGATGAAGACTACCTTGGACAAGCAAGGCATATG
CTCTCCAAAGTGGGAATGTGGGATTTTGACATTTTCTTGTTTGATCGCTTGACAAATGGA
AACAGCCTGGTAACACTGTTGTGCCACCTCTTCAATACCCATGGACTCATTCACCATTTC
AAGTTAGATATGGTGACCTTACACCGATTTTTAGTCATGGTTCAAGAAGATTACCACAGC
CAAAACCCGTATCACAATGCTGTTCACGCAGCCGACGTCACCCAGGCCATGCACTGCTAC
CTGAAAGAGCCAAAGCTTGCCAGCTTCCTCACGCCTCTGGACATCATGCTTGGACTGCTG
GCTGCAGCAGCACACGATGTGGACCACCCAGGGGTGAACCAGCCATTTTTGATAAAAACT
AACCACCATCTTGCAAACCTATATCAGAATATGTCTGTGCTGGAGAATCATCACTGGCGA
TCTACAATTGGCATGCTTCGAGAATCAAGGCTTCTTGCTCATTTGCCAAAGGAAATGACA
CAGGATATTGAACAGCAGCTGGGCTCCTTGATCTTGGCAACAGACATCAACAGGCAGAAT
GAATTTTTGACCAGATTGAAAGCTCACCTCCACAATAAAGACTTAAGACTGGAGGATGCA
CAGGACAGGCACTTTATGCTTCAGATCGCCTTGAAGTGTGCTGACATTTGCAATCCTTGT
AGAATCTGGGAGATGAGCAAGCAGTGGAGTGAAAGGGTCTGTGAAGAATTCTACAGGCAA
GGTGAACTTGAACAGAAATTTGAACTGGAAATCAGTCCTCTTTGTAATCAACAGAAAGAT
TCCATCCCTAGTATACAAATTGGTTTCATGAGCTACATCGTGGAGCCGCTCTTCCGGGAA
TGGGCCCATTTCACGGGTAACAGCACCCTGTCGGAGAACATGCTGGGCCACCTCGCACAC
AACAAGGCCCAGTGGAAGAGCCTGTTGCCCAGGCAGCACAGAAGCAGGGGCAGCAGTGGC
AGCGGGCCTGACCACGACCACGCAGGCCAAGGGACTGAGAGCGAGGAGCAGGAAGGCGAC
AGCCCC

ORF Protein

Cloned ORF protein sequence for pFN21ASDB0003 Download

>KIBB0003  502 aa
MPVLERYFHPAELGRRWTGPEGVLPSSPGSRPGCQQGPLPWDLPEMIRMVKLVWKSKSEL
QATKQRGILDNEDALRSFPGDIRLRGQTGVRAERRGSYPFIDFRLLNSTTYSGEIGTKKK
VKRLLSFQRYFHASRLLRGIIPQAPLHLLDEDYLGQARHMLSKVGMWDFDIFLFDRLTNG
NSLVTLLCHLFNTHGLIHHFKLDMVTLHRFLVMVQEDYHSQNPYHNAVHAADVTQAMHCY
LKEPKLASFLTPLDIMLGLLAAAAHDVDHPGVNQPFLIKTNHHLANLYQNMSVLENHHWR
STIGMLRESRLLAHLPKEMTQDIEQQLGSLILATDINRQNEFLTRLKAHLHNKDLRLEDA
QDRHFMLQIALKCADICNPCRIWEMSKQWSERVCEEFYRQGELEQKFELEISPLCNQQKD
SIPSIQIGFMSYIVEPLFREWAHFTGNSTLSENMLGHLAHNKAQWKSLLPRQHRSRGSSG
SGPDHDHAGQGTESEEQEGDSP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDB0003 Download

>pFN21ASDB0003  6235 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGCCTGTGCTAGAGCG
ATATTTCCACCCAGCAGAGCTAGGCAGGAGGTGGACAGGCCCAGAAGGTGTGCTGCCCTC
CTCCCCGGGAAGCCGGCCGGGGTGCCAGCAGGGGCCGCTGCCCTGGGACTTGCCAGAGAT
GATCAGGATGGTAAAGCTGGTTTGGAAATCCAAAAGTGAGCTGCAGGCGACCAAACAGAG
AGGCATTCTGGACAATGAAGATGCTCTCCGCAGCTTTCCAGGAGATATACGACTAAGGGG
TCAGACGGGGGTTCGTGCTGAACGCCGTGGCTCCTACCCATTCATTGACTTCCGCCTACT
TAACAGTACAACATACTCAGGGGAGATTGGCACCAAGAAAAAGGTGAAAAGACTATTAAG
CTTTCAAAGATACTTCCATGCATCAAGGCTGCTTCGTGGAATTATACCACAAGCCCCTCT
GCACCTGCTGGATGAAGACTACCTTGGACAAGCAAGGCATATGCTCTCCAAAGTGGGAAT
GTGGGATTTTGACATTTTCTTGTTTGATCGCTTGACAAATGGAAACAGCCTGGTAACACT
GTTGTGCCACCTCTTCAATACCCATGGACTCATTCACCATTTCAAGTTAGATATGGTGAC
CTTACACCGATTTTTAGTCATGGTTCAAGAAGATTACCACAGCCAAAACCCGTATCACAA
TGCTGTTCACGCAGCCGACGTCACCCAGGCCATGCACTGCTACCTGAAAGAGCCAAAGCT
TGCCAGCTTCCTCACGCCTCTGGACATCATGCTTGGACTGCTGGCTGCAGCAGCACACGA
TGTGGACCACCCAGGGGTGAACCAGCCATTTTTGATAAAAACTAACCACCATCTTGCAAA
CCTATATCAGAATATGTCTGTGCTGGAGAATCATCACTGGCGATCTACAATTGGCATGCT
TCGAGAATCAAGGCTTCTTGCTCATTTGCCAAAGGAAATGACACAGGATATTGAACAGCA
GCTGGGCTCCTTGATCTTGGCAACAGACATCAACAGGCAGAATGAATTTTTGACCAGATT
GAAAGCTCACCTCCACAATAAAGACTTAAGACTGGAGGATGCACAGGACAGGCACTTTAT
GCTTCAGATCGCCTTGAAGTGTGCTGACATTTGCAATCCTTGTAGAATCTGGGAGATGAG
CAAGCAGTGGAGTGAAAGGGTCTGTGAAGAATTCTACAGGCAAGGTGAACTTGAACAGAA
ATTTGAACTGGAAATCAGTCCTCTTTGTAATCAACAGAAAGATTCCATCCCTAGTATACA
AATTGGTTTCATGAGCTACATCGTGGAGCCGCTCTTCCGGGAATGGGCCCATTTCACGGG
TAACAGCACCCTGTCGGAGAACATGCTGGGCCACCTCGCACACAACAAGGCCCAGTGGAA
GAGCCTGTTGCCCAGGCAGCACAGAAGCAGGGGCAGCAGTGGCAGCGGGCCTGACCACGA
CCACGCAGGCCAAGGGACTGAGAGCGAGGAGCAGGAAGGCGACAGCCCCTACGTAGTTTA
AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG
ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT
AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG
TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT
GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC
CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT
GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT
TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC
CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC
TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG
GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT
CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC
ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA
CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA
AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT
GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT
TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT
GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG
CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG
ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC
GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT
TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG
CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG
AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA
CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT
GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC
CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA
GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG
TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA
CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA
CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA
TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC
CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT
TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA
GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC
GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT