(Circular) (Six-base) MAPSORT of: pFN21ASDB0003.seq Check: 2981 from: 1 to: 6235 pFN21ASDB0003 6235 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2472 2472 Size: 6235 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2721 4045 278 Size: 53 83 186 936 1185 1324 2468 Fragments arranged by size: 2468 1324 1185 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3295 3578 1937 Size: 1358 283 4594 Fragments arranged by size: 4594 1358 283 Acc65I G'GTAC_C Cuts at: 3556 3556 Size: 6235 AclI AA'CG_TT Cuts at: 1563 4362 4735 1563 Size: 2799 373 3063 Fragments arranged by size: 3063 2799 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3107 4298 5682 5950 1632 Size: 1475 1191 1384 268 1917 Fragments arranged by size: 1917 1475 1384 1191 268 AflII C'TTAA_G Cuts at: 820 1017 3085 820 Size: 197 2068 3970 Fragments arranged by size: 3970 2068 197 AflIII A'CryG_T Cuts at: 1203 5134 6111 1203 Size: 3931 977 1327 Fragments arranged by size: 3931 1327 977 AgeI A'CCGG_T Cuts at: 5066 5066 Size: 6235 AhdI GACnn_n'nnGTC Cuts at: 4964 4964 Size: 6235 AleI CACnn'nnGTG Cuts at: 1166 2581 1166 Size: 1415 4820 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6235 AlwNI CAG_nnn'CTG Cuts at: 3001 3978 5550 3001 Size: 977 1572 3686 Fragments arranged by size: 3686 1572 977 ApaI G_GGCC'C Cuts at: 3349 3349 Size: 6235 ApaLI G'TGCA_C Cuts at: 4292 5448 4292 Size: 1156 5079 ApoI r'AATT_y Cuts at: 1493 3044 3209 3239 3544 3770 3922 6031 1493 Size: 1551 165 30 305 226 152 2109 1697 Fragments arranged by size: 2109 1697 1551 305 226 165 152 30 AseI AT'TA_AT Cuts at: 160 1032 4789 160 Size: 872 3757 1606 Fragments arranged by size: 3757 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 AvaI C'yCGr_G Cuts at: 1870 1943 2104 3560 1870 Size: 73 161 1456 4545 Fragments arranged by size: 4545 1456 161 73 BamHI G'GATC_C Cuts at: 3565 3932 3565 Size: 367 5868 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2121 2369 3556 5011 618 Size: 325 217 627 334 248 1187 1455 1842 Fragments arranged by size: 1842 1455 1187 627 334 325 248 217 BanII G_rGCy'C Cuts at: 1391 3008 3349 3554 1391 Size: 1617 341 205 4072 Fragments arranged by size: 4072 1617 341 205 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2481 928 Size: 971 582 4682 Fragments arranged by size: 4682 971 582 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4426 4460 1903 Size: 34 2489 34 3678 Fragments arranged by size: 3678 2489 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4127 5337 4127 Size: 1210 5025 BclI T'GATC_A Cuts at: 1548 2160 1548 Size: 612 5623 BfrBI ATG'CAT Cuts at: 2420 2420 Size: 6235 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4846 136 Size: 107 122 71 631 605 3174 1525 Fragments arranged by size: 3174 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6230 6230 Size: 6235 BlpI GC'TnA_GC Cuts at: 3652 3652 Size: 6235 Bme1580I G_kGCm'C Cuts at: 1790 3349 4296 5452 1790 Size: 1559 947 1156 2573 Fragments arranged by size: 2573 1559 1156 947 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6235 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3406 3966 4924 Size: 416 53 83 399 24 1989 560 958 Cuts at: 4924 442 Size: 1753 Fragments arranged by size: 1989 1753 958 560 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6235 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2924 3113 4895 1239 Size: 1685 189 1782 2579 Fragments arranged by size: 2579 1782 1685 189 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4360 5225 5523 4360 Size: 865 298 5072 Fragments arranged by size: 5072 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4898 882 Size: 617 3399 2219 Fragments arranged by size: 3399 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3532 4026 493 Size: 711 2328 494 2702 Fragments arranged by size: 2702 2328 711 494 BsaBI GATnn'nnATC Cuts at: 6229 6229 Size: 6235 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2718 4042 4424 5059 Size: 53 83 186 936 1185 1324 382 635 Cuts at: 5059 275 Size: 1451 Fragments arranged by size: 1451 1324 1185 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4668 5066 5340 5487 5898 4668 Size: 398 274 147 411 5005 Fragments arranged by size: 5005 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6010 Fragments arranged by size: 6010 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2089 3521 1186 Size: 903 1432 3900 Fragments arranged by size: 3900 1432 903 BseYI C'CCAG_C Cuts at: 2050 3001 3385 5438 5934 2050 Size: 951 384 2053 496 2351 Fragments arranged by size: 2351 2053 951 496 384 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2444 1163 Size: 573 708 4954 Fragments arranged by size: 4954 708 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2117 3685 4446 5474 1540 Size: 275 186 116 1568 761 1028 2301 Fragments arranged by size: 2301 1568 1028 761 275 186 116 BsiHKAI G_wGCw'C Cuts at: 4296 4381 5452 4296 Size: 85 1071 5079 Fragments arranged by size: 5079 1071 85 BsmI GAATG_Cn' Cuts at: 2223 3746 3839 2223 Size: 1523 93 4619 Fragments arranged by size: 4619 1523 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3008 3349 3554 4296 4381 5452 1391 Size: 399 1218 341 205 742 85 1071 2174 Fragments arranged by size: 2174 1218 1071 742 399 341 205 85 BspEI T'CCGG_A Cuts at: 5898 5898 Size: 6235 BspHI T'CATG_A Cuts at: 3309 4124 5854 3309 Size: 815 1730 3690 Fragments arranged by size: 3690 1730 815 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2472 3590 844 Size: 1628 1118 3489 Fragments arranged by size: 3489 1628 1118 BsrBI CCG'CTC Cuts at: 1394 1823 3331 4122 1394 Size: 429 1508 791 3507 Fragments arranged by size: 3507 1508 791 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4730 4904 65 Size: 1183 3482 174 1396 Fragments arranged by size: 3482 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2112 4879 5066 1700 Size: 110 302 2767 187 2869 Fragments arranged by size: 2869 2767 302 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6235 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6235 BssSI C'ACGA_G Cuts at: 4295 5307 4295 Size: 1012 5223 BstAPI GCAn_nnn'nTGC Cuts at: 794 3118 794 Size: 2324 3911 BstBI TT'CG_AA Cuts at: 2007 5039 2007 Size: 3032 3203 BstEII G'GTnAC_C Cuts at: 1184 2635 1184 Size: 1451 4784 BstYI r'GATC_y Cuts at: 1972 3565 3932 4317 4334 5786 5872 5884 6230 Size: 1593 367 385 17 1452 86 12 346 Cuts at: 6230 1972 Size: 1977 Fragments arranged by size: 1977 1593 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3296 3296 Size: 6235 BtgI C'CryG_G Cuts at: 513 1065 2305 2602 513 Size: 552 1240 297 4146 Fragments arranged by size: 4146 1240 552 297 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5496 Fragments arranged by size: 5496 683 56 BtsI GCAGTG_nn' Cuts at: 2734 3191 3463 3751 4545 4565 2734 Size: 457 272 288 794 20 4404 Fragments arranged by size: 4404 794 457 288 272 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6200 DraI TTT'AAA Cuts at: 3539 3539 Size: 6235 DraIII CAC_nnn'GTG Cuts at: 2821 2821 Size: 6235 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2277 5242 809 Size: 507 961 2965 1802 Fragments arranged by size: 2965 1802 961 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2114 3682 4571 Size: 54 1425 50 275 18 284 1568 889 Cuts at: 4571 6200 8 Size: 1629 43 Fragments arranged by size: 1629 1568 1425 889 284 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2114 3682 1537 Size: 275 302 1568 4090 Fragments arranged by size: 4090 1568 302 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2597 3338 3413 4165 1353 Size: 1244 741 75 752 3423 Fragments arranged by size: 3423 1244 752 741 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2321 4822 5196 5342 387 Size: 1064 138 732 2501 374 146 1280 Fragments arranged by size: 2501 1280 1064 732 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2924 3107 3113 4298 4895 5682 5950 Size: 393 1292 183 6 1185 597 787 268 Cuts at: 5950 1239 Size: 1524 Fragments arranged by size: 1524 1292 1185 787 597 393 268 183 6 EcoRI G'AATT_C Cuts at: 3209 3544 3922 3209 Size: 335 378 5522 Fragments arranged by size: 5522 378 335 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2498 3245 2498 Size: 747 5488 FspI TGC'GCA Cuts at: 4741 4741 Size: 6235 HaeII r_GCGC'y Cuts at: 1678 5382 1678 Size: 3704 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2640 2672 4876 4908 4950 Size: 32 135 32 1068 32 2204 32 42 Cuts at: 4950 4982 1373 Size: 32 2626 Fragments arranged by size: 2626 2204 1068 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3579 3825 669 Size: 988 281 1641 246 3079 Fragments arranged by size: 3079 1641 988 281 246 HindIII A'AGCT_T Cuts at: 748 2398 2756 748 Size: 1650 358 4227 Fragments arranged by size: 4227 1650 358 HpaI GTT'AAC Cuts at: 3825 3825 Size: 6235 KpnI G_GTAC'C Cuts at: 3560 3560 Size: 6235 MfeI C'AATT_G Cuts at: 2928 3834 2928 Size: 906 5329 MluI A'CGCG_T Cuts at: 6111 6111 Size: 6235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3354 5349 5533 1305 Size: 2049 1995 184 2007 Fragments arranged by size: 2049 2007 1995 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4413 Fragments arranged by size: 4413 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2581 4193 4552 4711 518 Size: 648 861 554 1612 359 159 2042 Fragments arranged by size: 2042 1612 861 648 554 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 3001 3465 3651 4328 5476 5721 1606 Size: 531 864 464 186 677 1148 245 2120 Fragments arranged by size: 2120 1148 864 677 531 464 245 186 NaeI GCC'GGC Cuts at: 2114 2114 Size: 6235 NcoI C'CATG_G Cuts at: 513 1065 2602 513 Size: 552 1537 4146 Fragments arranged by size: 4146 1537 552 NdeI CA'TA_TG Cuts at: 387 2499 387 Size: 2112 4123 NgoMIV G'CCGG_C Cuts at: 2112 2112 Size: 6235 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6235 NotI GC'GGCC_GC Cuts at: 3682 3682 Size: 6235 NsiI A_TGCA'T Cuts at: 2422 2422 Size: 6235 NspI r_CATG'y Cuts at: 1132 1229 2938 3385 3593 5138 1132 Size: 97 1709 447 208 1545 2229 Fragments arranged by size: 2229 1709 1545 447 208 97 PasI CC'CwG_GG Cuts at: 1348 2142 2831 1348 Size: 794 689 4752 Fragments arranged by size: 4752 794 689 PciI A'CATG_T Cuts at: 5134 5134 Size: 6235 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6235 PfoI T'CCnGG_A Cuts at: 2257 3336 2257 Size: 1079 5156 PmeI GTTT'AAAC Cuts at: 3539 3539 Size: 6235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4304 4336 5817 5849 4304 Size: 32 1481 32 4690 Fragments arranged by size: 4690 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6235 PsiI TTA'TAA Cuts at: 3805 3805 Size: 6235 PspOMI G'GGCC_C Cuts at: 3345 3345 Size: 6235 PstI C_TGCA'G Cuts at: 830 2205 2809 3587 830 Size: 1375 604 778 3478 Fragments arranged by size: 3478 1375 778 604 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6235 PvuII CAG'CTG Cuts at: 3001 3001 Size: 6235 SalI G'TCGA_C Cuts at: 1936 3577 1936 Size: 1641 4594 SapI GCTCTTCn'nnn_ Cuts at: 3338 3413 3338 Size: 75 6160 SbfI CC_TGCA'GG Cuts at: 3587 3587 Size: 6235 ScaI AGT'ACT Cuts at: 4483 6195 4483 Size: 1712 4523 SfcI C'TryA_G Cuts at: 826 1045 2201 2805 3214 3583 4718 5399 5590 Size: 219 1156 604 409 369 1135 681 191 Cuts at: 5590 826 Size: 1471 Fragments arranged by size: 1471 1156 1135 681 604 409 369 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6235 SmaI CCC'GGG Cuts at: 1872 2106 3562 1872 Size: 234 1456 4545 Fragments arranged by size: 4545 1456 234 SmlI C'TyrA_G Cuts at: 820 1017 1943 3085 4339 5240 5502 820 Size: 197 926 1142 1254 901 262 1553 Fragments arranged by size: 1553 1254 1142 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3532 4026 493 Size: 3039 494 2702 Fragments arranged by size: 3039 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6235 SphI G_CATG'C Cuts at: 1132 2938 3593 1132 Size: 1806 655 3774 Fragments arranged by size: 3774 1806 655 SspI AAT'ATT Cuts at: 5 52 4159 5 Size: 47 4107 2081 Fragments arranged by size: 4107 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6235 StyI C'CwwG_G Cuts at: 513 1065 2482 2602 3490 3674 513 Size: 552 1417 120 888 184 3074 Fragments arranged by size: 3074 1417 888 552 184 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2064 2738 2844 4262 4279 4432 4617 Size: 1255 484 674 106 1418 17 153 185 Cuts at: 4617 5062 325 Size: 445 1498 Fragments arranged by size: 1498 1418 1255 674 484 445 185 153 106 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2345 4481 6193 Size: 274 80 33 51 1443 368 2136 1712 Cuts at: 6193 96 Size: 138 Fragments arranged by size: 2136 1712 1443 368 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6195 6227 6195 Size: 32 6203 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6235 XbaI T'CTAG_A Cuts at: 3571 3571 Size: 6235 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6235 XmaI C'CCGG_G Cuts at: 1870 2104 3560 1870 Size: 234 1456 4545 Fragments arranged by size: 4545 1456 234 XmnI GAAnn'nnTTC Cuts at: 4364 4364 Size: 6235 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2719 4043 276 Size: 53 83 186 936 1185 1324 2468 Fragments arranged by size: 2468 1324 1185 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI