GRWD1 ORF clone pFN21ASDA1942

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Halotag ORF Clone pFN21ASDA1942

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Clone info.

Species

human

Clone Name

pFN21ASDA1942 Sequence (with vector) Restriction Map

Product ID

FHC00951

Flexi Clone PID

FXC00951

ORF Sequence

DNA sequence (1338 bp)
Protein sequence (446 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384328]

Gene

KIAA1942

Symbol

GRWD1

Alias : CDW4, GRWD, RRB1, WDR28

Description

glutamate-rich WD repeat containing 1

Original Clone ID

fg00781

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1338 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 446 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12720.1 4.3e-125 99.8 GRWD1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR020472 277 291 PR00320 G-protein beta WD-40 repeat IPR020472 323 337 PR00320 G-protein beta WD-40 repeat IPR020472 369 383 PR00320 G-protein beta WD-40 repeat HMMPfam IPR022052 43 112 PF12265 Histone-binding protein RBBP4 IPR019781 205 242 PF00400 WD40 repeat IPR019781 257 290 PF00400 WD40 repeat IPR019781 302 336 PF00400 WD40 repeat IPR019781 343 382 PF00400 WD40 repeat HMMSmart IPR001680 203 243 SM00320 WD40 repeat IPR001680 249 290 SM00320 WD40 repeat IPR001680 297 336 SM00320 WD40 repeat IPR001680 342 382 SM00320 WD40 repeat ProfileScan IPR017986 210 391 PS50294 WD40-repeat-containing domain IPR019782 257 292 PS50082 WD40 repeat 2 IPR019782 304 338 PS50082 WD40 repeat 2 IPR019782 349 383 PS50082 WD40 repeat 2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_113673 446 610597 99.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1942 Download

>KIAA1942  1338 bp
ATGGCGGCGCGCAAGGGTCGGCGTCGCACGTGTGAAACCGGGGAACCCATGGAAGCCGAG
TCCGGCGACACAAGTTCCGAGGGCCCGGCCCAGGTCTACCTGCCCGGCCGGGGGCCGCCG
CTACGCGAAGGGGAGGAGCTGGTCATGGACGAGGAGGCCTATGTGCTCTACCACCGAGCG
CAGACTGGCGCCCCCTGTCTCAGCTTTGACATAGTCCGGGATCACCTGGGAGACAACCGG
ACAGAGCTTCCTCTTACACTTTACTTGTGTGCTGGGACCCAGGCTGAGAGCGCCCAGAGC
AACAGACTGATGATGCTTCGGATGCACAATCTGCATGGGACAAAGCCCCCACCCTCAGAG
GGCAGTGATGAAGAAGAAGAGGAGGAAGATGAAGAGGATGAAGAAGAGCGGAAACCTCAG
CTGGAGCTGGCCATGGTGCCCCACTATGGTGGCATCAACCGAGTTCGGGTGTCATGGCTG
GGTGAAGAGCCTGTGGCTGGGGTGTGGTCAGAGAAGGGCCAGGTGGAGGTGTTTGCGCTG
CGGCGGCTTCTGCAGGTGGTGGAGGAGCCCCAGGCCCTGGCAGCCTTCCTCCGGGATGAG
CAGGCCCAAATGAAGCCCATCTTCTCCTTCGCTGGACACATGGGCGAGGGCTTTGCCCTT
GACTGGTCCCCCCGGGTGACCGGTCGCCTGCTGACCGGTGACTGTCAAAAGAACATCCAC
CTCTGGACACCTACGGACGGCGGCTCCTGGCACGTGGACCAGCGGCCATTCGTGGGCCAC
ACACGCTCTGTGGAGGACCTGCAGTGGTCACCGACTGAGAACACGGTGTTTGCCTCCTGC
TCAGCTGACGCCTCCATCCGCATCTGGGACATCCGGGCAGCCCCCAGCAAGGCCTGCATG
CTCACCACAGCCACCGCCCATGATGGGGACGTCAATGTCATCAGCTGGAGCCGCCAGGAG
CCCTTCCTGCTCAGTGGCGGGGATGATGGGGCCCTCAAGATCTGGGACCTTCGGCAGTTC
AAGTCTGGTTCCCCAGTGGCCACCTTCAAGCAGCACGTGGCCCCCGTGACCTCCGTCGAG
TGGCACCCCCAGGACAGCGGGGTCTTTGCAGCCTCGGGTGCAGACCACCAGATCACACAG
TGGGACCTGGCAGTGGAGCGGGACCCTGAGGCGGGCGACGTGGAGGCCGACCCCGGACTG
GCCGACCTCCCGCAGCAGCTGCTGTTCGTGCACCAGGGCGAGACCGAGCTGAAGGAGCTG
CACTGGCACCCGCAGTGCCCAGGGCTCCTGGTCAGCACGGCGCTGTCAGGCTTCACCATC
TTCCGCACCATCAGCGTC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1942 Download

>KIAA1942  446 aa
MAARKGRRRTCETGEPMEAESGDTSSEGPAQVYLPGRGPPLREGEELVMDEEAYVLYHRA
QTGAPCLSFDIVRDHLGDNRTELPLTLYLCAGTQAESAQSNRLMMLRMHNLHGTKPPPSE
GSDEEEEEEDEEDEEERKPQLELAMVPHYGGINRVRVSWLGEEPVAGVWSEKGQVEVFAL
RRLLQVVEEPQALAAFLRDEQAQMKPIFSFAGHMGEGFALDWSPRVTGRLLTGDCQKNIH
LWTPTDGGSWHVDQRPFVGHTRSVEDLQWSPTENTVFASCSADASIRIWDIRAAPSKACM
LTTATAHDGDVNVISWSRQEPFLLSGGDDGALKIWDLRQFKSGSPVATFKQHVAPVTSVE
WHPQDSGVFAASGADHQITQWDLAVERDPEAGDVEADPGLADLPQQLLFVHQGETELKEL
HWHPQCPGLLVSTALSGFTIFRTISV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1942 Download

>pFN21ASDA1942  6067 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCGGCGCGCAAGGG
TCGGCGTCGCACGTGTGAAACCGGGGAACCCATGGAAGCCGAGTCCGGCGACACAAGTTC
CGAGGGCCCGGCCCAGGTCTACCTGCCCGGCCGGGGGCCGCCGCTACGCGAAGGGGAGGA
GCTGGTCATGGACGAGGAGGCCTATGTGCTCTACCACCGAGCGCAGACTGGCGCCCCCTG
TCTCAGCTTTGACATAGTCCGGGATCACCTGGGAGACAACCGGACAGAGCTTCCTCTTAC
ACTTTACTTGTGTGCTGGGACCCAGGCTGAGAGCGCCCAGAGCAACAGACTGATGATGCT
TCGGATGCACAATCTGCATGGGACAAAGCCCCCACCCTCAGAGGGCAGTGATGAAGAAGA
AGAGGAGGAAGATGAAGAGGATGAAGAAGAGCGGAAACCTCAGCTGGAGCTGGCCATGGT
GCCCCACTATGGTGGCATCAACCGAGTTCGGGTGTCATGGCTGGGTGAAGAGCCTGTGGC
TGGGGTGTGGTCAGAGAAGGGCCAGGTGGAGGTGTTTGCGCTGCGGCGGCTTCTGCAGGT
GGTGGAGGAGCCCCAGGCCCTGGCAGCCTTCCTCCGGGATGAGCAGGCCCAAATGAAGCC
CATCTTCTCCTTCGCTGGACACATGGGCGAGGGCTTTGCCCTTGACTGGTCCCCCCGGGT
GACCGGTCGCCTGCTGACCGGTGACTGTCAAAAGAACATCCACCTCTGGACACCTACGGA
CGGCGGCTCCTGGCACGTGGACCAGCGGCCATTCGTGGGCCACACACGCTCTGTGGAGGA
CCTGCAGTGGTCACCGACTGAGAACACGGTGTTTGCCTCCTGCTCAGCTGACGCCTCCAT
CCGCATCTGGGACATCCGGGCAGCCCCCAGCAAGGCCTGCATGCTCACCACAGCCACCGC
CCATGATGGGGACGTCAATGTCATCAGCTGGAGCCGCCAGGAGCCCTTCCTGCTCAGTGG
CGGGGATGATGGGGCCCTCAAGATCTGGGACCTTCGGCAGTTCAAGTCTGGTTCCCCAGT
GGCCACCTTCAAGCAGCACGTGGCCCCCGTGACCTCCGTCGAGTGGCACCCCCAGGACAG
CGGGGTCTTTGCAGCCTCGGGTGCAGACCACCAGATCACACAGTGGGACCTGGCAGTGGA
GCGGGACCCTGAGGCGGGCGACGTGGAGGCCGACCCCGGACTGGCCGACCTCCCGCAGCA
GCTGCTGTTCGTGCACCAGGGCGAGACCGAGCTGAAGGAGCTGCACTGGCACCCGCAGTG
CCCAGGGCTCCTGGTCAGCACGGCGCTGTCAGGCTTCACCATCTTCCGCACCATCAGCGT
CTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT