Restriction map for
pFN21ASDA1942
(Circular) (Six-base) MAPSORT of: pFN21ASDA1942.seq Check: 864 from: 1 to: 6067
pFN21ASDA1942 6067 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2566 2566
Size: 6067
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2955 3877 278
Size: 53 83 186 936 1419 922 2468
Fragments arranged by size:
2468 1419 936 922 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2118 3360 3410 1937
Size: 181 1242 50 4594
Fragments arranged by size:
4594 1242 181 50
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3293 4130 5514 5782 1632
Size: 1661 837 1384 268 1917
Fragments arranged by size:
1917 1661 1384 837 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 2051 4966 5943 1203
Size: 848 2915 977 1327
Fragments arranged by size:
2915 1327 977 848
AgeI A'CCGG_T
Cuts at: 2702 2717 4898 2702
Size: 15 2181 3871
Fragments arranged by size:
3871 2181 15
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 2469 1166
Size: 1303 4764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 2599 3241 3244 3810 5382 2599
Size: 642 3 566 1572 3284
Fragments arranged by size:
3284 1572 642 566 3
ApaI G_GGCC'C
Cuts at: 2108 3016 2108
Size: 908 5159
ApaLI G'TGCA_C
Cuts at: 3251 4124 5280 3251
Size: 873 1156 4038
Fragments arranged by size:
4038 1156 873
ApoI r'AATT_y
Cuts at: 1493 3376 3602 3754 5863 1493
Size: 1883 226 152 2109 1697
Fragments arranged by size:
2109 1883 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 2694 3136 3392 1870
Size: 73 751 442 256 4545
Fragments arranged by size:
4545 751 442 256 73
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2210 2458 3105 3288 3388
Size: 325 217 627 423 248 647 183 100
Cuts at: 3388 4843 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 647 627 423 325 248 217
183 100
BanII G_rGCy'C
Cuts at: 1391 2108 2592 2985 3016 3309 3386 1391
Size: 717 484 393 31 293 77 4072
Fragments arranged by size:
4072 717 484 393 293 77 31
BbeI G_GCGC'C
Cuts at: 2214 2214
Size: 6067
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5096
BbvCI CC'TCA_GC
Cuts at: 2439 2439
Size: 6067
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2657 2691 4258 4292 1903
Size: 34 720 34 1567 34 3678
Fragments arranged by size:
3678 1567 720 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5169 3959
Size: 1210 4857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4678 136
Size: 107 122 71 631 605 3006 1525
Fragments arranged by size:
3006 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3021 6062 3021
Size: 3041 3026
BlpI GC'TnA_GC
Cuts at: 2863 3484 2863
Size: 621 5446
Bme1580I G_kGCm'C
Cuts at: 1790 2108 2463 3016 3255 3302 4128 5284 1790
Size: 318 355 553 239 47 826 1156 2573
Fragments arranged by size:
2573 1156 826 553 355 318 239 47
BmgBI CAC'GTC
Cuts at: 1318 3202 1318
Size: 1884 4183
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3050 3798 4756
Size: 416 53 83 399 24 1633 748 958
Cuts at: 4756 442
Size: 1753
Fragments arranged by size:
1753 1633 958 748 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2465 2989 4727 1239
Size: 1226 524 1738 2579
Fragments arranged by size:
2579 1738 1226 524
Bpu10I CC'TnA_GC
Cuts at: 2439 2439
Size: 6067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3002 4192 5057 5355 3002
Size: 1190 865 298 3714
Fragments arranged by size:
3714 1190 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3257 4730 882
Size: 617 1758 1473 2219
Fragments arranged by size:
2219 1758 1473 617
BsaAI yAC'GTr
Cuts at: 493 1204 2052 2776 3079 3364 3858 493
Size: 711 848 724 303 285 494 2702
Fragments arranged by size:
2702 848 724 711 494 303 285
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2044 2211 2871 2952
Size: 53 83 186 936 511 167 660 81
Cuts at: 2952 3874 4256 4891 275
Size: 922 382 635 1451
Fragments arranged by size:
1451 936 922 660 635 511 382 186
167 83 81 53
BsaWI w'CCGG_w
Cuts at: 2259 2702 2717 4500 4898 5172 5319 5730 2259
Size: 443 15 1783 398 274 147 411 2596
Fragments arranged by size:
2596 1783 443 411 398 274 147 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2750 2780 1178
Size: 30 165 30 1347 30 4465
Fragments arranged by size:
4465 1347 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 2189 2417 2600 1186
Size: 985 18 228 183 4653
Fragments arranged by size:
4653 985 228 183 18
BseYI C'CCAG_C
Cuts at: 2294 2500 2519 2906 5270 5766 2294
Size: 206 19 387 2364 496 2595
Fragments arranged by size:
2595 2364 496 387 206 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3162 3266 1163
Size: 573 1426 104 3964
Fragments arranged by size:
3964 1426 573 104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2131 2707 3517 4278 5306 1540
Size: 275 186 130 576 810 761 1028 2301
Fragments arranged by size:
2301 1028 810 761 576 275 186 130
BsiHKAI G_wGCw'C
Cuts at: 2190 3255 4128 4213 5284 2190
Size: 1065 873 85 1071 2973
Fragments arranged by size:
2973 1071 1065 873 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2190 2463 2592 2985 3016 3255
Size: 399 318 82 273 129 393 31 239
Cuts at: 3255 3302 3309 3386 4128 4213 5284 1391
Size: 47 7 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 393 318 273 239
129 85 82 77 47 31 7
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 3956 5686 3956
Size: 1730 4337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2130 2566 2829 3422 844
Size: 1286 436 263 593 3489
Fragments arranged by size:
3489 1286 593 436 263
BsrBI CCG'CTC
Cuts at: 1394 1823 2431 3181 3954 1394
Size: 429 608 750 773 3507
Fragments arranged by size:
3507 773 750 608 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2702 2717 4711 4898 1700
Size: 110 892 15 1994 187 2869
Fragments arranged by size:
2869 1994 892 187 110 15
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6067
BssHII G'CGCG_C
Cuts at: 1925 2030 1925
Size: 105 5962
BssSI C'ACGA_G
Cuts at: 4127 5139 4127
Size: 1012 5055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2353 2916 3241 794
Size: 1559 563 325 3620
Fragments arranged by size:
3620 1559 563 325
BstBI TT'CG_AA
Cuts at: 2007 4871 2007
Size: 2864 3203
BstEII G'GTnAC_C
Cuts at: 1184 2698 2829 1184
Size: 1514 131 4422
Fragments arranged by size:
4422 1514 131
BstXI CCAn_nnnn'nTGG
Cuts at: 2471 2471
Size: 6067
BstYI r'GATC_y
Cuts at: 1972 3021 3397 3764 4149 4166 5618 5704 5716
Size: 1049 376 367 385 17 1452 86 12
Cuts at: 5716 6062 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1049 385 376 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3189 3189
Size: 6067
BtgI C'CryG_G
Cuts at: 513 1065 2022 2070 2454 513
Size: 552 957 48 384 4126
Fragments arranged by size:
4126 957 552 384 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 2392 2831 3180 3302 3583 4377 4397 2392
Size: 439 349 122 281 794 20 4062
Fragments arranged by size:
4062 794 439 349 281 122 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DraIII CAC_nnn'GTG
Cuts at: 3162 3162
Size: 6067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2957 3122 5074 809
Size: 507 1641 165 1952 1802
Fragments arranged by size:
1952 1802 1641 507 165
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2128 2451 2786
Size: 54 1425 50 275 18 298 323 335
Cuts at: 2786 3060 3222 3514 4403 6032 8
Size: 274 162 292 889 1629 43
Fragments arranged by size:
1629 1425 889 335 323 298 292 275
274 162 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2128 3514 1537
Size: 275 316 1386 4090
Fragments arranged by size:
4090 1386 316 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2394 2409 2421 2502 3997 1353
Size: 1041 15 12 81 1495 3423
Fragments arranged by size:
3423 1495 1041 81 15 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4654 5028 5174 387
Size: 1064 138 3065 374 146 1280
Fragments arranged by size:
3065 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2465 2989 3293 4130 4727 5514 5782
Size: 393 833 524 304 837 597 787 268
Cuts at: 5782 1239
Size: 1524
Fragments arranged by size:
1524 837 833 787 597 524 393 304
268
EcoO109I rG'GnC_Cy
Cuts at: 2104 2298 2596 2818 3012 3013 3028 3166 3184
Size: 194 298 222 194 1 15 138 18
Cuts at: 3184 2104
Size: 4987
Fragments arranged by size:
4987 298 222 194 194 138 18 15
1
EcoRI G'AATT_C
Cuts at: 3376 3754 3376
Size: 378 5689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2659 2659
Size: 6067
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 2214 2316 3326 5214 1678
Size: 536 102 1010 1888 2531
Fragments arranged by size:
2531 1888 1010 536 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2335 2367 2750 2782 4708
Size: 32 135 32 763 32 383 32 1926
Cuts at: 4708 4740 4782 4814 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1926 763 383 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3657 669
Size: 988 281 1473 246 3079
Fragments arranged by size:
3079 1473 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KasI G'GCGC_C
Cuts at: 2210 2210
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 3666 3666
Size: 6067
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5181 5365 1305
Size: 3876 184 2007
Fragments arranged by size:
3876 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2453 3062 10
Size: 54 1425 343 621 609 3015
Fragments arranged by size:
3015 1425 621 609 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2469 4025 4384 4543 518
Size: 648 1303 1556 359 159 2042
Fragments arranged by size:
2042 1556 1303 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2443 2785 2867 2967 3120 3241 3483 4160
Size: 837 342 82 100 153 121 242 677
Cuts at: 4160 5308 5553 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 837 677 342 245 242 153
121 100 82
NarI GG'CG_CC
Cuts at: 2211 2211
Size: 6067
NcoI C'CATG_G
Cuts at: 513 1065 2022 2070 2454 513
Size: 552 957 48 384 4126
Fragments arranged by size:
4126 957 552 384 48
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6067
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 2923 3425 4970 1132
Size: 97 1694 502 1545 2229
Fragments arranged by size:
2229 1694 1545 502 97
PasI CC'CwG_GG
Cuts at: 1348 3302 1348
Size: 1954 4113
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2599 1108
Size: 1491 4576
PfoI T'CCnGG_A
Cuts at: 2238 2613 2238
Size: 375 5692
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PmlI CAC'GTG
Cuts at: 2052 2776 3079 2052
Size: 724 303 5040
Fragments arranged by size:
5040 724 303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2298 2818 3028 3166 3184 2298
Size: 520 210 138 18 5181
Fragments arranged by size:
5181 520 210 138 18
PshAI GACnn'nnGTC
Cuts at: 1613 3095 1613
Size: 1482 4585
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PspOMI G'GGCC_C
Cuts at: 2104 3012 2104
Size: 908 5159
PstI C_TGCA'G
Cuts at: 830 2577 2826 3419 830
Size: 1747 249 593 3478
Fragments arranged by size:
3478 1747 593 249
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2443 2867 2967 3241 2443
Size: 424 100 274 5269
Fragments arranged by size:
5269 424 274 100
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SanDI GG'GwC_CC
Cuts at: 2298 3184 2298
Size: 886 5181
SapI GCTCTTCn'nnn_
Cuts at: 2421 2502 2421
Size: 81 5986
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SfcI C'TryA_G
Cuts at: 826 1045 2573 2822 3415 4550 5231 5422 826
Size: 219 1528 249 593 1135 681 191 1471
Fragments arranged by size:
1528 1471 1135 681 593 249 219 191
SfoI GGC'GCC
Cuts at: 2212 2212
Size: 6067
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 2696 3394 1872
Size: 824 698 4545
Fragments arranged by size:
4545 824 698
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3017 4171 5072 5334 820
Size: 197 926 1074 1154 901 262 1553
Fragments arranged by size:
1553 1154 1074 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3364 3858 493
Size: 2871 494 2702
Fragments arranged by size:
2871 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 2923 3425 1132
Size: 1791 502 3774
Fragments arranged by size:
3774 1791 502
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 2180 2915 1517
Size: 663 735 4669
Fragments arranged by size:
4669 735 663
StyI C'CwwG_G
Cuts at: 513 1065 2022 2070 2454 3506 513
Size: 552 957 48 384 1052 3074
Fragments arranged by size:
3074 1052 957 552 384 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2492 3281 4094 4111 4264 4449 4894
Size: 1255 912 789 813 17 153 185 445
Cuts at: 4894 325
Size: 1498
Fragments arranged by size:
1498 1255 912 813 789 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4313 6025 96
Size: 274 80 33 51 1443 2336 1712 138
Fragments arranged by size:
2336 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2810 2842 6027 6059 2810
Size: 32 3185 32 2818
Fragments arranged by size:
3185 2818 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2234 1316
Size: 918 5149
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6067
XmaI C'CCGG_G
Cuts at: 1870 2694 3392 1870
Size: 824 698 4545
Fragments arranged by size:
4545 824 698
XmnI GAAnn'nnTTC
Cuts at: 4196 4196
Size: 6067
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2953 3875 276
Size: 53 83 186 936 1419 922 2468
Fragments arranged by size:
2468 1419 936 922 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI
SacII SexAI SfiI SrfI SwaI XcmI