ARHGEF4 ORF clone pFN21ASDA1112

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Halotag ORF Clone pFN21ASDA1112

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Clone info.

Species

human

Clone Name

pFN21ASDA1112 Sequence (with vector) Restriction Map

Product ID

FHC00742

Flexi Clone PID

FXC00742

ORF Sequence

DNA sequence (2010 bp)
Protein sequence (670 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384116]

Gene

KIAA1112

Symbol

ARHGEF4

Alias : ASEF, ASEF1, GEF4, SMIM39, STM6

Description

Rho guanine nucleotide exchange factor (GEF) 4, transcript variant 2

Original Clone ID

hj05505s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2010 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 670 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS42754.1 2.1e-203 100.0 ARHGEF4 CCDS2165.1 9.8e-195 96.2 ARHGEF4 CCDS9305.1 4.7e-95 60.5 SPATA13 CCDS53857.1 7.8e-95 60.5 SPATA13 CCDS66517.1 1.2e-93 61.1 SPATA13 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001452 201 245 PF00018 Src homology-3 domain IPR000219 288 467 PF00621 Dbl homology (DH) domain IPR001849 501 601 PF00169 Pleckstrin homology domain HMMSmart IPR001452 197 252 SM00326 Src homology-3 domain IPR000219 288 467 SM00325 Dbl homology (DH) domain IPR001849 500 608 SM00233 Pleckstrin homology domain ProfileScan IPR001452 201 253 PS50002 Src homology-3 domain IPR000219 284 468 PS50010 Dbl homology (DH) domain IPR001849 499 606 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_127462 670 605216 100.0 100.0 NP_056135 690 605216 96.2 100.0 XP_005263738 1876 605216 96.2 100.0 XP_011509576 1870 605216 99.2 96.7 NP_694568 652 613324 60.5 82.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1112 Download

>KIAA1112  2010 bp
ATGCCCTGGGAAGAACCAGCAGGTGAGAAGCCCAGTTGCTCTCACAGTCAGAAGGCGTTC
CACATGGAGCCTGCCCAGAAGCCCTGCTTCACCACTGACATGGTGACATGGGCCCTCCTC
TGCATCTCTGCAGAGACTGTGCGTGGGGAGGCTCCTTCACAGCCTAGGGGCATCCCTCAC
CGCTCGCCCGTCAGTGTGGATGACCTGTGGCTGGAGAAGACACAGAGAAAGAAGTTGCAG
AAGCAGGCCCACGTCGAAAGGAGGCTGCACATAGGGGCAGTGCACAAAGATGGAGTCAAG
TGCTGGAGAAAGACGATCATTACCTCTCCAGAGTCTTTGAATCTCCCTAGAAGAAGCCAT
CCACTCTCCCAGAGTGCTCCAACGGGACTGAACCACATGGGCTGGCCAGAGCACACACCA
GGCACTGCCATGCCTGATGGAGCTCTGGACACAGCTGTCTGCGCTGACGAAGTGGGGAGC
GAGGAGGACCTGTATGATGACCTGCACAGCTCCAGCCACCACTACAGCCACCCTGGAGGG
GGTGGGGAGCAGCTGGCTATCAATGAGCTCATCAGCGATGGCAGTGTGGTCTGCGCTGAA
GCACTCTGGGACCATGTCACCATGGACGACCAGGAGCTGGGCTTCAAAGCTGGGGACGTC
ATCGAAGTGATGGATGCCACCAACAGAGAGTGGTGGTGGGGCCGGGTCGCCGATGGCGAG
GGCTGGTTTCCAGCCAGCTTCGTTCGGCTGAGGGTGAATCAGGACGAGCCCGCGGATGAC
GACGCCCCTCTGGCCGGGAACAGCGGAGCGGAGGACGGCGGGGCGGAGGCGCAGAGCAGC
AAGGACCAGATGCGGACCAACGTCATCAACGAGATCCTCAGCACTGAGCGGGACTACATC
AAGCACCTGCGCGACATCTGCGAGGGCTACGTCCGGCAGTGCCGCAAGCGCGCAGACATG
TTCAGCGAGGAGCAGCTGCGTACCATCTTCGGGAACATCGAGGACATCTACCGCTGCCAG
AAGGCCTTCGTGAAGGCCCTGGAGCAGAGGTTCAACCGCGAGCGCCCACACCTGAGCGAG
CTGGGTGCCTGCTTCCTGGAGCATCAAGCCGACTTCCAGATCTACTCGGAGTACTGCAAT
AACCACCCCAACGCCTGCGTGGAGCTCTCCCGGCTCACCAAGCTCAGCAAGTACGTGTAC
TTCTTCGAGGCCTGCCGGCTGCTGCAGAAGATGATTGACATCTCCCTGGATGGCTTCCTG
CTGACTCCGGTGCAGAAGATCTGCAAGTACCCTCTGCAGCTGGCCGAGCTGCTCAAATAC
ACGCACCCCCAGCACAGGGACTTCAAGGATGTTGAAGCCGCCTTGCATGCCATGAAGAAC
GTGGCCCAGCTCATCAACGAGCGGAAGCGGAGACTTGAGAACATCGACAAGATTGCTCAG
TGGCAGAGCTCCATAGAGGACTGGGAGGGAGAAGATCTCTTGGTCAGGAGCTCAGAACTC
ATCTACTCGGGGGAGCTGACTCGAGTTACACAGCCTCAAGCCAAAAGCCAGCAGCGAATG
TTCTTTCTCTTTGACCACCAGCTCATCTACTGTAAGAAGGACCTGCTCCGCCGCGACGTG
TTGTACTACAAGGGCCGGCTGGACATGGACGGCCTGGAGGTGGTGGACCTGGAGGACGGG
AAGGACAGAGACCTCCATGTGAGCATCAAGAACGCCTTCCGGCTGCACCGTGGCGCCACA
GGGGACAGCCACCTGCTGTGCACCAGGAAGCCCGAGCAGAAGCAGCGCTGGCTCAAGGCC
TTTGCCAGGGAGAGGGAGCAGGTGCAGCTGGACCAGGAGACAGGCTTCTCCATCACTGAA
CTGCAGAGGAAGCAGGCCATGCTGAATGCCAGCAAGCAGCAGGTCACAGGGAAGCCCAAA
GGTAGGCGGACAGCAGCCCCACCTCCTCGGCTGCCCGGTCCTTACCCTGCTGACATCATT
CCCTTCTCTGAACCACAAAGCCAAGCCAGC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1112 Download

>KIAA1112  670 aa
MPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPH
RSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSH
PLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGG
GGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGE
GWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYI
KHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSE
LGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFL
LTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQ
WQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDV
LYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKA
FAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKGRRTAAPPPRLPGPYPADII
PFSEPQSQAS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1112 Download

>pFN21ASDA1112  6739 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGCCCTGGGAAGAACC
AGCAGGTGAGAAGCCCAGTTGCTCTCACAGTCAGAAGGCGTTCCACATGGAGCCTGCCCA
GAAGCCCTGCTTCACCACTGACATGGTGACATGGGCCCTCCTCTGCATCTCTGCAGAGAC
TGTGCGTGGGGAGGCTCCTTCACAGCCTAGGGGCATCCCTCACCGCTCGCCCGTCAGTGT
GGATGACCTGTGGCTGGAGAAGACACAGAGAAAGAAGTTGCAGAAGCAGGCCCACGTCGA
AAGGAGGCTGCACATAGGGGCAGTGCACAAAGATGGAGTCAAGTGCTGGAGAAAGACGAT
CATTACCTCTCCAGAGTCTTTGAATCTCCCTAGAAGAAGCCATCCACTCTCCCAGAGTGC
TCCAACGGGACTGAACCACATGGGCTGGCCAGAGCACACACCAGGCACTGCCATGCCTGA
TGGAGCTCTGGACACAGCTGTCTGCGCTGACGAAGTGGGGAGCGAGGAGGACCTGTATGA
TGACCTGCACAGCTCCAGCCACCACTACAGCCACCCTGGAGGGGGTGGGGAGCAGCTGGC
TATCAATGAGCTCATCAGCGATGGCAGTGTGGTCTGCGCTGAAGCACTCTGGGACCATGT
CACCATGGACGACCAGGAGCTGGGCTTCAAAGCTGGGGACGTCATCGAAGTGATGGATGC
CACCAACAGAGAGTGGTGGTGGGGCCGGGTCGCCGATGGCGAGGGCTGGTTTCCAGCCAG
CTTCGTTCGGCTGAGGGTGAATCAGGACGAGCCCGCGGATGACGACGCCCCTCTGGCCGG
GAACAGCGGAGCGGAGGACGGCGGGGCGGAGGCGCAGAGCAGCAAGGACCAGATGCGGAC
CAACGTCATCAACGAGATCCTCAGCACTGAGCGGGACTACATCAAGCACCTGCGCGACAT
CTGCGAGGGCTACGTCCGGCAGTGCCGCAAGCGCGCAGACATGTTCAGCGAGGAGCAGCT
GCGTACCATCTTCGGGAACATCGAGGACATCTACCGCTGCCAGAAGGCCTTCGTGAAGGC
CCTGGAGCAGAGGTTCAACCGCGAGCGCCCACACCTGAGCGAGCTGGGTGCCTGCTTCCT
GGAGCATCAAGCCGACTTCCAGATCTACTCGGAGTACTGCAATAACCACCCCAACGCCTG
CGTGGAGCTCTCCCGGCTCACCAAGCTCAGCAAGTACGTGTACTTCTTCGAGGCCTGCCG
GCTGCTGCAGAAGATGATTGACATCTCCCTGGATGGCTTCCTGCTGACTCCGGTGCAGAA
GATCTGCAAGTACCCTCTGCAGCTGGCCGAGCTGCTCAAATACACGCACCCCCAGCACAG
GGACTTCAAGGATGTTGAAGCCGCCTTGCATGCCATGAAGAACGTGGCCCAGCTCATCAA
CGAGCGGAAGCGGAGACTTGAGAACATCGACAAGATTGCTCAGTGGCAGAGCTCCATAGA
GGACTGGGAGGGAGAAGATCTCTTGGTCAGGAGCTCAGAACTCATCTACTCGGGGGAGCT
GACTCGAGTTACACAGCCTCAAGCCAAAAGCCAGCAGCGAATGTTCTTTCTCTTTGACCA
CCAGCTCATCTACTGTAAGAAGGACCTGCTCCGCCGCGACGTGTTGTACTACAAGGGCCG
GCTGGACATGGACGGCCTGGAGGTGGTGGACCTGGAGGACGGGAAGGACAGAGACCTCCA
TGTGAGCATCAAGAACGCCTTCCGGCTGCACCGTGGCGCCACAGGGGACAGCCACCTGCT
GTGCACCAGGAAGCCCGAGCAGAAGCAGCGCTGGCTCAAGGCCTTTGCCAGGGAGAGGGA
GCAGGTGCAGCTGGACCAGGAGACAGGCTTCTCCATCACTGAACTGCAGAGGAAGCAGGC
CATGCTGAATGCCAGCAAGCAGCAGGTCACAGGGAAGCCCAAAGGTAGGCGGACAGCAGC
CCCACCTCCTCGGCTGCCCGGTCCTTACCCTGCTGACATCATTCCCTTCTCTGAACCACA
AAGCCAAGCCAGCTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT