(Circular) (Six-base) MAPSORT of: pFN21ASDA1112.seq Check: 908 from: 1 to: 6739 pFN21ASDA1112 6739 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2033 2937 3783 3832 2033 Size: 904 846 49 4940 Fragments arranged by size: 4940 904 846 49 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2682 4549 278 Size: 53 83 186 936 1146 1867 2468 Fragments arranged by size: 2468 1867 1146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4082 1937 Size: 2145 4594 Acc65I G'GTAC_C Cuts at: 4060 4060 Size: 6739 AclI AA'CG_TT Cuts at: 1563 4866 5239 1563 Size: 3303 373 3063 Fragments arranged by size: 3303 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2640 4802 6186 6454 1632 Size: 1008 2162 1384 268 1917 Fragments arranged by size: 2162 1917 1384 1008 268 AfeI AGC'GCT Cuts at: 3809 3809 Size: 6739 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6542 AflIII A'CryG_T Cuts at: 1203 2979 3216 3639 5638 6615 1203 Size: 1776 237 423 1999 977 1327 Fragments arranged by size: 1999 1776 1327 977 423 237 AgeI A'CCGG_T Cuts at: 5570 5570 Size: 6739 AhdI GACnn_n'nnGTC Cuts at: 5468 5468 Size: 6739 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6739 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2468 1184 Size: 1284 5455 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3056 3088 3056 Size: 32 6707 AlwNI CAG_nnn'CTG Cuts at: 2159 2447 2659 4482 6054 2159 Size: 288 212 1823 1572 2844 Fragments arranged by size: 2844 1823 1572 288 212 ApaI G_GGCC'C Cuts at: 2137 2137 Size: 6739 ApaLI G'TGCA_C Cuts at: 2303 3781 4796 5952 2303 Size: 1478 1015 1156 3090 Fragments arranged by size: 3090 1478 1156 1015 ApoI r'AATT_y Cuts at: 1493 4048 4274 4426 6535 1493 Size: 2555 226 152 2109 1697 Fragments arranged by size: 2555 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5293 160 Size: 872 4261 1606 Fragments arranged by size: 4261 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 AvaI C'yCGr_G Cuts at: 1870 1943 3529 3543 3794 4064 1870 Size: 73 1586 14 251 270 4545 Fragments arranged by size: 4545 1586 270 251 73 14 AvrII C'CTAG_G Cuts at: 2186 2186 Size: 6739 BamHI G'GATC_C Cuts at: 4069 4436 4069 Size: 367 6372 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3107 3755 4060 5515 618 Size: 325 217 627 1320 648 305 1455 1842 Fragments arranged by size: 1842 1455 1320 648 627 325 305 217 BanII G_rGCy'C Cuts at: 1391 2137 2467 2592 2793 3189 3473 3515 4058 Size: 746 330 125 201 396 284 42 543 Cuts at: 4058 1391 Size: 4072 Fragments arranged by size: 4072 746 543 396 330 284 201 125 42 BbeI G_GCGC'C Cuts at: 3759 3759 Size: 6739 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2246 928 Size: 971 347 5421 Fragments arranged by size: 5421 971 347 BbvCI CC'TCA_GC Cuts at: 2771 2900 2771 Size: 129 6610 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3089 3123 3437 3471 4930 4964 1903 Size: 34 1152 34 314 34 1459 34 3678 Fragments arranged by size: 3678 1459 1152 314 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4631 5841 4631 Size: 1210 5529 BclI T'GATC_A Cuts at: 1548 1548 Size: 6739 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2813 5350 136 Size: 107 122 71 631 605 1141 2537 1525 Fragments arranged by size: 2537 1525 1141 631 605 122 107 71 BglII A'GATC_T Cuts at: 3141 3300 3496 6734 3141 Size: 159 196 3238 3146 Fragments arranged by size: 3238 3146 196 159 BlpI GC'TnA_GC Cuts at: 3206 4156 3206 Size: 950 5789 Bme1580I G_kGCm'C Cuts at: 1790 2137 2307 3785 4800 5956 1790 Size: 347 170 1478 1015 1156 2573 Fragments arranged by size: 2573 1478 1156 1015 347 170 BmgBI CAC'GTC Cuts at: 1318 2275 3640 1318 Size: 957 1365 4417 Fragments arranged by size: 4417 1365 957 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2049 3493 4470 Size: 416 53 83 399 24 632 1444 977 Cuts at: 4470 5428 442 Size: 958 1753 Fragments arranged by size: 1753 1444 977 958 632 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6739 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3515 3515 Size: 6739 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2255 2334 2347 2518 2577 3083 3140 3698 Size: 1016 79 13 171 59 506 57 558 Cuts at: 3698 3713 5399 1239 Size: 15 1686 2579 Fragments arranged by size: 2579 1686 1016 558 506 171 79 59 57 15 13 Bpu10I CC'TnA_GC Cuts at: 2771 2900 3095 2771 Size: 129 195 6415 Fragments arranged by size: 6415 195 129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3458 3543 3800 4864 5729 6027 3458 Size: 85 257 1064 865 298 4170 Fragments arranged by size: 4170 1064 865 298 257 85 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3705 5402 882 Size: 617 2206 1697 2219 Fragments arranged by size: 2219 2206 1697 617 BsaAI yAC'GTr Cuts at: 493 1204 3217 4036 4530 493 Size: 711 2013 819 494 2702 Fragments arranged by size: 2702 2013 819 711 494 BsaBI GATnn'nnATC Cuts at: 3260 6733 3260 Size: 3473 3266 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2679 2805 3756 4546 Size: 53 83 186 936 1146 126 951 790 Cuts at: 4546 4928 5563 275 Size: 382 635 1451 Fragments arranged by size: 1451 1146 951 936 790 635 382 186 126 83 53 BsaWI w'CCGG_w Cuts at: 3289 5172 5570 5844 5991 6402 3289 Size: 1883 398 274 147 411 3626 Fragments arranged by size: 3626 1883 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2498 2528 3056 3086 1178 Size: 30 165 30 1095 30 528 30 4831 Fragments arranged by size: 4831 1095 528 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2519 3005 3957 1186 Size: 943 390 486 952 3968 Fragments arranged by size: 3968 952 943 486 390 BseYI C'CCAG_C Cuts at: 2659 2672 3103 3351 3408 5942 6438 2659 Size: 13 431 248 57 2534 496 2960 Fragments arranged by size: 2960 2534 496 431 248 57 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2273 2510 3314 3731 3866 1163 Size: 573 537 237 804 417 135 4036 Fragments arranged by size: 4036 804 573 537 417 237 135 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4189 4950 5978 1540 Size: 275 186 2188 761 1028 2301 Fragments arranged by size: 2301 2188 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2307 2401 2436 2467 2592 3189 3473 3515 3785 Size: 94 35 31 125 597 284 42 270 Cuts at: 3785 4800 4885 5956 2307 Size: 1015 85 1071 3090 Fragments arranged by size: 3090 1071 1015 597 284 270 125 94 85 42 35 31 BsmI GAATG_Cn' Cuts at: 3913 4250 4343 3913 Size: 337 93 6309 Fragments arranged by size: 6309 337 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2137 2307 2401 2436 2467 2592 2793 Size: 399 347 170 94 35 31 125 201 Cuts at: 2793 3189 3473 3515 3785 4058 4800 4885 5956 Size: 396 284 42 270 273 742 85 1071 Cuts at: 5956 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 396 347 284 273 270 201 170 125 94 85 42 35 31 BspEI T'CCGG_A Cuts at: 6402 6402 Size: 6739 BspHI T'CATG_A Cuts at: 4628 6358 4628 Size: 1730 5009 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2033 2532 2937 3633 3783 3832 3913 4094 Size: 1189 499 405 696 150 49 81 181 Cuts at: 4094 844 Size: 3489 Fragments arranged by size: 3489 1189 696 499 405 181 150 81 49 BsrBI CCG'CTC Cuts at: 1394 1823 2205 2831 2911 3424 4626 1394 Size: 429 382 626 80 513 1202 3507 Fragments arranged by size: 3507 1202 626 513 429 382 80 BsrDI GCAATG_nn' Cuts at: 65 1248 5234 5408 65 Size: 1183 3986 174 1396 Fragments arranged by size: 3986 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3237 3657 5383 5570 1700 Size: 110 1427 420 1726 187 2869 Fragments arranged by size: 2869 1726 1427 420 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6739 BssHII G'CGCG_C Cuts at: 1925 2971 1925 Size: 1046 5693 BssSI C'ACGA_G Cuts at: 4799 5811 4799 Size: 1012 5727 BstAPI GCAn_nnn'nTGC Cuts at: 794 3901 794 Size: 3107 3632 BstBI TT'CG_AA Cuts at: 2007 5543 2007 Size: 3536 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6739 BstXI CCAn_nnnn'nTGG Cuts at: 2122 2122 Size: 6739 BstYI r'GATC_y Cuts at: 1972 2895 3141 3300 3496 4069 4436 4821 4838 Size: 923 246 159 196 573 367 385 17 Cuts at: 4838 6290 6376 6388 6734 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 923 573 385 367 346 246 196 159 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2643 2793 3751 513 Size: 552 1578 150 958 3501 Fragments arranged by size: 3501 1578 958 552 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2613 487 Size: 683 56 1387 4613 Fragments arranged by size: 4613 1387 683 56 BtsI GCAGTG_nn' Cuts at: 2307 2445 2611 2966 4255 5049 5069 2307 Size: 138 166 355 1289 794 20 3977 Fragments arranged by size: 3977 1289 794 355 166 138 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6739 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6704 DraI TTT'AAA Cuts at: 4043 4043 Size: 6739 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2477 5746 809 Size: 507 1161 3269 1802 Fragments arranged by size: 3269 1802 1161 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2426 2814 3324 Size: 54 1425 50 275 18 596 388 510 Cuts at: 3324 4186 5075 6704 8 Size: 862 889 1629 43 Fragments arranged by size: 1629 1425 889 862 596 510 388 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4186 1537 Size: 275 2374 4090 Fragments arranged by size: 4090 2374 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4669 1353 Size: 3316 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2861 3620 3964 5326 5700 5846 Size: 1064 138 1272 759 344 1362 374 146 Cuts at: 5846 387 Size: 1280 Fragments arranged by size: 1362 1280 1272 1064 759 374 344 146 138 EcoICRI GAG'CTC Cuts at: 2465 2590 3187 3471 3513 2465 Size: 125 597 284 42 5691 Fragments arranged by size: 5691 597 284 125 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2334 2347 2518 2577 2640 3083 Size: 393 623 79 13 171 59 63 443 Cuts at: 3083 3140 3698 3713 4802 5399 6186 6454 1239 Size: 57 558 15 1089 597 787 268 1524 Fragments arranged by size: 1524 1089 787 623 597 558 443 393 268 171 79 63 59 57 15 13 EcoNI CCTnn'n_nnAGG Cuts at: 3826 3826 Size: 6739 EcoO109I rG'GnC_Cy Cuts at: 2134 2509 3058 3622 2134 Size: 375 549 564 5251 Fragments arranged by size: 5251 564 549 375 EcoRI G'AATT_C Cuts at: 4048 4426 4048 Size: 378 6361 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2125 3754 2125 Size: 1629 5110 FspI TGC'GCA Cuts at: 5245 5245 Size: 6739 HaeII r_GCGC'y Cuts at: 1678 3088 3759 3811 5886 1678 Size: 1410 671 52 2075 2531 Fragments arranged by size: 2531 2075 1410 671 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2764 2796 5380 5412 5454 Size: 32 135 32 1192 32 2584 32 42 Cuts at: 5454 5486 1373 Size: 32 2626 Fragments arranged by size: 2626 2584 1192 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4083 4329 669 Size: 988 281 2145 246 3079 Fragments arranged by size: 3079 2145 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6739 HpaI GTT'AAC Cuts at: 4329 4329 Size: 6739 KasI G'GCGC_C Cuts at: 3755 3755 Size: 6739 KpnI G_GTAC'C Cuts at: 4064 4064 Size: 6739 MfeI C'AATT_G Cuts at: 4338 4338 Size: 6739 MluI A'CGCG_T Cuts at: 6615 6615 Size: 6739 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2426 5853 6037 1305 Size: 1121 3427 184 2007 Fragments arranged by size: 3427 2007 1121 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2428 10 Size: 54 1425 343 596 4321 Fragments arranged by size: 4321 1425 596 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2310 2450 2687 4697 5056 5215 Size: 648 954 190 140 237 2010 359 159 Cuts at: 5215 518 Size: 2042 Fragments arranged by size: 2042 2010 954 648 359 237 190 159 140 MspA1I CmG'CkG Cuts at: 1606 2477 2575 2795 2826 2998 3036 3322 3850 Size: 871 98 220 31 172 38 286 528 Cuts at: 3850 4155 4832 5980 6225 1606 Size: 305 677 1148 245 2120 Fragments arranged by size: 2120 1148 871 677 528 305 286 245 220 172 98 38 31 NaeI GCC'GGC Cuts at: 3239 3659 3239 Size: 420 6319 NarI GG'CG_CC Cuts at: 3756 3756 Size: 6739 NcoI C'CATG_G Cuts at: 513 1065 2643 513 Size: 552 1578 4609 Fragments arranged by size: 4609 1578 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6739 NgoMIV G'CCGG_C Cuts at: 3237 3657 3237 Size: 420 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6739 NotI GC'GGCC_GC Cuts at: 4186 4186 Size: 6739 NspI r_CATG'y Cuts at: 1132 1229 2983 3392 4097 5642 1132 Size: 97 1754 409 705 1545 2229 Fragments arranged by size: 2229 1754 1545 705 409 97 PasI CC'CwG_GG Cuts at: 1348 2028 1348 Size: 680 6059 PciI A'CATG_T Cuts at: 2979 5638 2979 Size: 2659 4080 PflMI CCAn_nnn'nTGG Cuts at: 1108 2028 2659 1108 Size: 920 631 5188 Fragments arranged by size: 5188 920 631 PfoI T'CCnGG_A Cuts at: 3117 3117 Size: 6739 PmeI GTTT'AAAC Cuts at: 4043 4043 Size: 6739 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3056 3088 4808 4840 6321 6353 3056 Size: 32 1720 32 1481 32 3442 Fragments arranged by size: 3442 1720 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2509 3622 2509 Size: 1113 5626 PshAI GACnn'nnGTC Cuts at: 1613 2882 1613 Size: 1269 5470 PsiI TTA'TAA Cuts at: 4309 4309 Size: 6739 PspOMI G'GGCC_C Cuts at: 2133 2133 Size: 6739 PspXI vC'TCGA_Gb Cuts at: 3543 3543 Size: 6739 PstI C_TGCA'G Cuts at: 830 2155 3249 3321 3888 4091 830 Size: 1325 1094 72 567 203 3478 Fragments arranged by size: 3478 1325 1094 567 203 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6739 PvuII CAG'CTG Cuts at: 2477 2575 2998 3322 3850 2477 Size: 98 423 324 528 5366 Fragments arranged by size: 5366 528 423 324 98 SacI G_AGCT'C Cuts at: 2467 2592 3189 3473 3515 2467 Size: 125 597 284 42 5691 Fragments arranged by size: 5691 597 284 125 42 SacII CC_GC'GG Cuts at: 2796 2796 Size: 6739 SalI G'TCGA_C Cuts at: 1936 4081 1936 Size: 2145 4594 SbfI CC_TGCA'GG Cuts at: 4091 4091 Size: 6739 ScaI AGT'ACT Cuts at: 3155 4987 6699 3155 Size: 1832 1712 3195 Fragments arranged by size: 3195 1832 1712 SfcI C'TryA_G Cuts at: 826 1045 2151 2545 3245 3317 3884 4087 5222 Size: 219 1106 394 700 72 567 203 1135 Cuts at: 5222 5903 6094 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1106 700 681 567 394 219 203 191 72 SfoI GGC'GCC Cuts at: 3757 3757 Size: 6739 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6739 SmaI CCC'GGG Cuts at: 1872 4066 1872 Size: 2194 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3437 3543 3558 3815 4843 5744 Size: 197 926 1494 106 15 257 1028 901 Cuts at: 5744 6006 820 Size: 262 1553 Fragments arranged by size: 1553 1494 1028 926 901 262 257 197 106 15 SnaBI TAC'GTA Cuts at: 493 4036 4530 493 Size: 3543 494 2702 Fragments arranged by size: 3543 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6739 SphI G_CATG'C Cuts at: 1132 3392 4097 1132 Size: 2260 705 3774 Fragments arranged by size: 3774 2260 705 SspI AAT'ATT Cuts at: 5 52 4663 5 Size: 47 4611 2081 Fragments arranged by size: 4611 2081 47 StuI AGG'CCT Cuts at: 1517 3047 3233 3821 1517 Size: 1530 186 588 4435 Fragments arranged by size: 4435 1530 588 186 StyI C'CwwG_G Cuts at: 513 1065 2186 2643 4178 513 Size: 552 1121 457 1535 3074 Fragments arranged by size: 3074 1535 1121 552 457 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3095 4766 4783 4936 5121 5566 325 Size: 1255 1515 1671 17 153 185 445 1498 Fragments arranged by size: 1671 1515 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3153 3219 3645 Size: 274 80 33 51 1443 1176 66 426 Cuts at: 3645 4985 6697 96 Size: 1340 1712 138 Fragments arranged by size: 1712 1443 1340 1176 426 274 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2213 2245 6699 6731 2213 Size: 32 4454 32 2221 Fragments arranged by size: 4454 2221 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2636 1316 Size: 1320 5419 XbaI T'CTAG_A Cuts at: 4075 4075 Size: 6739 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3178 3400 3178 Size: 222 6517 XhoI C'TCGA_G Cuts at: 1943 3543 1943 Size: 1600 5139 XmaI C'CCGG_G Cuts at: 1870 4064 1870 Size: 2194 4545 XmnI GAAnn'nnTTC Cuts at: 2078 3047 3737 4868 2078 Size: 969 690 1131 3949 Fragments arranged by size: 3949 1131 969 690 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2680 4547 276 Size: 53 83 186 936 1146 1867 2468 Fragments arranged by size: 2468 1867 1146 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI