METTL13 ORF clone pFN21ASDA0859

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Halotag ORF Clone pFN21ASDA0859

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Clone info.

Species

human

Clone Name

pFN21ASDA0859 Sequence (with vector) Restriction Map

Product ID

FHC01611

Flexi Clone PID

FXC01611

ORF Sequence

DNA sequence (2097 bp)
Protein sequence (699 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384020]

Gene

KIAA0859

Symbol

METTL13

Alias : 5630401D24Rik, CGI-01, DFNB26, DFNB26M, DFNM1, EEF1AKNMT, KIAA0859, feat

Description

methyltransferase like 13, transcript variant 1

Original Clone ID

hk06485s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2097 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 699 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1299.1 0 100.0 METTL13 CCDS1300.1 0 100.0 METTL13 CCDS30936.1 5.5e-170 77.7 METTL13 CCDS3255.1 1.9e-21 34.6 ECE2 CCDS3256.2 7.2e-18 35.1 ECE2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013216 53 157 PF08241 Methyltransferase type 11 IPR001045 518 613 PF01564 Spermine synthase Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0859 Download

>KIAA0859  2097 bp
ATGAACCTCTTACCTAAAAGTTCCAGGGAGTTTGGCTCCGTTGACTATTGGGAGAAGTTC
TTCCAGCAGCGAGGAAAGAAAGCTTTCGAGTGGTATGGAACCTACCTGGAACTGTGCGGG
GTGCTACATAAATATATCAAGCCCAGGGAAAAGGTGCTGGTGATTGGGTGTGGCAACTCA
GAACTGAGTGAGCAACTGTATGATGTGGGCTATCGGGATATAGTGAACATCGACATCAGT
GAGGTTGTCATCAAGCAAATGAAGGAATGTAATGCCACCCGACGGCCCCAGATGAGCTTC
TTGAAGATGGACATGACGCAGATGGAGTTTCCTGATGCCTCGTTCCAGGTGGTGTTGGAC
AAGGGCACCCTGGATGCTGTCCTGACAGATGAGGAAGAGAAGACCTTACAACAGGTGGAC
AGGATGCTGGCTGAGGTTGGCCGTGTCCTGCAGGTGGGCGGTCGCTATCTCTGCATCTCC
CTGGCTCAGGCTCACATCCTGAAGAAAGCAGTGGGCCACTTCTCCCGGGAGGGGTGGATG
GTGAGGGTGCACCAAGTGGCCAACAGCCAGGACCAGGTGTTGGAAGCAGAGCCTCAGTTC
TCCTTGCCTGTCTTTGCCTTCATCATGACCAAGTTCAGGCCAGTCCCTGGCTCTGCCCTT
CAGATCTTTGAGCTGTGTGCTCAGGAGCAGCGCAAGCCTGTGCGGCTGGAGAGTGCCGAG
CGGCTGGCCGAGGCGGTGCAGGAGCGACAGCAGTATGCCTGGCTGTGCAGCCAGCTGCGC
CGCAAGGCCAGGCTGGGGAGTGTGTCTCTGGACTTGTGCGATGGGGACACGGGGGAGCCA
CGCTACACCCTCCACGTGGTGGACAGCCCCACTGTGAAACCATCGCGGGACAATCATTTT
GCGATTTTCATCATCCCTCAGGGCCGGGAGACCGAGTGGCTCTTTGGCATGGATGAGGGC
CGGAAACAGCTGGCGGCCAGTGCTGGCTTCAGGAGGTTGATTACAGTGGCCCTTCACCGA
GGTCAGCAGTATGAAAGCATGGACCACATCCAAGCTGAGCTGTCGGCTAGAGTCATGGAG
CTGGCCCCAGCTGGGATGCCCACCCAGCAGCAGGTCCCCTTTCTGTCTGTGGGTGGGGAC
ATTGGGGTCCGGACCGTTCAGCACCAAGACTGCAGCCCCTTGAGCGGTGACTATGTCATT
GAGGATGTGCAAGGGGATGACAAGCGATACTTCCGTCGACTGATCTTCCTCAGCAACAGG
AATGTGGTGCAGTCCGAAGCCAGGTTGCTGAAGGATGTGTCTCACAAAGCCCAGAAGAAG
CGGAAAAAGGACAGGAAGAAGCAGCGGCCTGCTGATGCGGAGGACCTCCCTGCAGCCCCG
GGGCAGTCCATTGATAAGAGTTACCTGTGTTGTGAACACCACAAAGCCATGATCGCTGGC
CTTGCCCTGCTGAGAAACCCAGAGCTACTCCTAGAGATCCCACTGGCATTGTTGGTGGTA
GGCCTGGGCGGGGGCAGCCTCCCCCTCTTTGTCCACGATCATTTTCCAAAGTCCTGCATT
GATGCTGTGGAGATCGATCCCTCCATGTTGGAAGTGGCCACCCAGTGGTTTGGCTTCTCC
CAGAGTGACCGAATGAAGGTCCACATTGCAGATGGCCTGGACTATATCGCCAGCTTGGCA
GGAGGAGGAGAAGCACGGCCTTGCTACGATGTCATAATGTTTGATGTTGACAGTAAGGAC
CCAACACTGGGAATGAGTTGTCCGCCCCCAGCATTTGTGGAGCAATCTTTTCTACAGAAG
GTTAAAAGCATCTTGACTCCTGAAGGTGTTTTTATTCTCAACCTTGTGTGCCGAGACTTG
GGGCTAAAAGACTCAGTGCTGGCTGGGCTCAAGGCAGTGTTCCCCCTCCTATATGTCCGG
CGAATTGAGGGTGAAGTGAATGAGATCCTGTTCTGTCAGCTGCACCCTGAGCAAAAACTT
GCCACACCAGAGCTCCTAGAAACAGCCCAGGCTTTGGAGCGGACCCTGAGGAAGCCTGGG
AGGGGTTGGGATGACACGTATGTCTTGTCAGATATGCTCAAGACGGTGAAAATTGTG

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0859 Download

>KIAA0859  699 aa
MNLLPKSSREFGSVDYWEKFFQQRGKKAFEWYGTYLELCGVLHKYIKPREKVLVIGCGNS
ELSEQLYDVGYRDIVNIDISEVVIKQMKECNATRRPQMSFLKMDMTQMEFPDASFQVVLD
KGTLDAVLTDEEEKTLQQVDRMLAEVGRVLQVGGRYLCISLAQAHILKKAVGHFSREGWM
VRVHQVANSQDQVLEAEPQFSLPVFAFIMTKFRPVPGSALQIFELCAQEQRKPVRLESAE
RLAEAVQERQQYAWLCSQLRRKARLGSVSLDLCDGDTGEPRYTLHVVDSPTVKPSRDNHF
AIFIIPQGRETEWLFGMDEGRKQLAASAGFRRLITVALHRGQQYESMDHIQAELSARVME
LAPAGMPTQQQVPFLSVGGDIGVRTVQHQDCSPLSGDYVIEDVQGDDKRYFRRLIFLSNR
NVVQSEARLLKDVSHKAQKKRKKDRKKQRPADAEDLPAAPGQSIDKSYLCCEHHKAMIAG
LALLRNPELLLEIPLALLVVGLGGGSLPLFVHDHFPKSCIDAVEIDPSMLEVATQWFGFS
QSDRMKVHIADGLDYIASLAGGGEARPCYDVIMFDVDSKDPTLGMSCPPPAFVEQSFLQK
VKSILTPEGVFILNLVCRDLGLKDSVLAGLKAVFPLLYVRRIEGEVNEILFCQLHPEQKL
ATPELLETAQALERTLRKPGRGWDDTYVLSDMLKTVKIV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0859 Download

>pFN21ASDA0859  6826 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGAACCTCTTACCTAA
AAGTTCCAGGGAGTTTGGCTCCGTTGACTATTGGGAGAAGTTCTTCCAGCAGCGAGGAAA
GAAAGCTTTCGAGTGGTATGGAACCTACCTGGAACTGTGCGGGGTGCTACATAAATATAT
CAAGCCCAGGGAAAAGGTGCTGGTGATTGGGTGTGGCAACTCAGAACTGAGTGAGCAACT
GTATGATGTGGGCTATCGGGATATAGTGAACATCGACATCAGTGAGGTTGTCATCAAGCA
AATGAAGGAATGTAATGCCACCCGACGGCCCCAGATGAGCTTCTTGAAGATGGACATGAC
GCAGATGGAGTTTCCTGATGCCTCGTTCCAGGTGGTGTTGGACAAGGGCACCCTGGATGC
TGTCCTGACAGATGAGGAAGAGAAGACCTTACAACAGGTGGACAGGATGCTGGCTGAGGT
TGGCCGTGTCCTGCAGGTGGGCGGTCGCTATCTCTGCATCTCCCTGGCTCAGGCTCACAT
CCTGAAGAAAGCAGTGGGCCACTTCTCCCGGGAGGGGTGGATGGTGAGGGTGCACCAAGT
GGCCAACAGCCAGGACCAGGTGTTGGAAGCAGAGCCTCAGTTCTCCTTGCCTGTCTTTGC
CTTCATCATGACCAAGTTCAGGCCAGTCCCTGGCTCTGCCCTTCAGATCTTTGAGCTGTG
TGCTCAGGAGCAGCGCAAGCCTGTGCGGCTGGAGAGTGCCGAGCGGCTGGCCGAGGCGGT
GCAGGAGCGACAGCAGTATGCCTGGCTGTGCAGCCAGCTGCGCCGCAAGGCCAGGCTGGG
GAGTGTGTCTCTGGACTTGTGCGATGGGGACACGGGGGAGCCACGCTACACCCTCCACGT
GGTGGACAGCCCCACTGTGAAACCATCGCGGGACAATCATTTTGCGATTTTCATCATCCC
TCAGGGCCGGGAGACCGAGTGGCTCTTTGGCATGGATGAGGGCCGGAAACAGCTGGCGGC
CAGTGCTGGCTTCAGGAGGTTGATTACAGTGGCCCTTCACCGAGGTCAGCAGTATGAAAG
CATGGACCACATCCAAGCTGAGCTGTCGGCTAGAGTCATGGAGCTGGCCCCAGCTGGGAT
GCCCACCCAGCAGCAGGTCCCCTTTCTGTCTGTGGGTGGGGACATTGGGGTCCGGACCGT
TCAGCACCAAGACTGCAGCCCCTTGAGCGGTGACTATGTCATTGAGGATGTGCAAGGGGA
TGACAAGCGATACTTCCGTCGACTGATCTTCCTCAGCAACAGGAATGTGGTGCAGTCCGA
AGCCAGGTTGCTGAAGGATGTGTCTCACAAAGCCCAGAAGAAGCGGAAAAAGGACAGGAA
GAAGCAGCGGCCTGCTGATGCGGAGGACCTCCCTGCAGCCCCGGGGCAGTCCATTGATAA
GAGTTACCTGTGTTGTGAACACCACAAAGCCATGATCGCTGGCCTTGCCCTGCTGAGAAA
CCCAGAGCTACTCCTAGAGATCCCACTGGCATTGTTGGTGGTAGGCCTGGGCGGGGGCAG
CCTCCCCCTCTTTGTCCACGATCATTTTCCAAAGTCCTGCATTGATGCTGTGGAGATCGA
TCCCTCCATGTTGGAAGTGGCCACCCAGTGGTTTGGCTTCTCCCAGAGTGACCGAATGAA
GGTCCACATTGCAGATGGCCTGGACTATATCGCCAGCTTGGCAGGAGGAGGAGAAGCACG
GCCTTGCTACGATGTCATAATGTTTGATGTTGACAGTAAGGACCCAACACTGGGAATGAG
TTGTCCGCCCCCAGCATTTGTGGAGCAATCTTTTCTACAGAAGGTTAAAAGCATCTTGAC
TCCTGAAGGTGTTTTTATTCTCAACCTTGTGTGCCGAGACTTGGGGCTAAAAGACTCAGT
GCTGGCTGGGCTCAAGGCAGTGTTCCCCCTCCTATATGTCCGGCGAATTGAGGGTGAAGT
GAATGAGATCCTGTTCTGTCAGCTGCACCCTGAGCAAAAACTTGCCACACCAGAGCTCCT
AGAAACAGCCCAGGCTTTGGAGCGGACCCTGAGGAAGCCTGGGAGGGGTTGGGATGACAC
GTATGTCTTGTCAGATATGCTCAAGACGGTGAAAATTGTGTACGTAGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT