(Circular) (Six-base) MAPSORT of: pFN21ASDA0859.seq Check: 1386 from: 1 to: 6826 pFN21ASDA0859 6826 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2464 2464 Size: 6826 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4636 278 Size: 53 83 186 936 3100 2468 Fragments arranged by size: 3100 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3259 4169 1937 Size: 1322 910 4594 Fragments arranged by size: 4594 1322 910 Acc65I G'GTAC_C Cuts at: 4147 4147 Size: 6826 AclI AA'CG_TT Cuts at: 1563 4953 5326 1563 Size: 3390 373 3063 Fragments arranged by size: 3390 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2543 2666 2995 3332 3864 4889 6273 6541 Size: 911 123 329 337 532 1025 1384 268 Cuts at: 6541 1632 Size: 1917 Fragments arranged by size: 1917 1384 1025 911 532 337 329 268 123 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6629 AflIII A'CryG_T Cuts at: 1203 4077 5725 6702 1203 Size: 2874 1648 977 1327 Fragments arranged by size: 2874 1648 1327 977 AgeI A'CCGG_T Cuts at: 5657 5657 Size: 6826 AhdI GACnn_n'nnGTC Cuts at: 5555 5555 Size: 6826 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6826 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2738 2813 3387 3739 4017 1184 Size: 1554 75 574 352 278 3993 Fragments arranged by size: 3993 1554 574 352 278 75 AlwNI CAG_nnn'CTG Cuts at: 2669 4569 6141 2669 Size: 1900 1572 3354 Fragments arranged by size: 3354 1900 1572 ApaLI G'TGCA_C Cuts at: 2570 4883 6039 2570 Size: 2313 1156 3357 Fragments arranged by size: 3357 2313 1156 ApoI r'AATT_y Cuts at: 1493 4135 4361 4513 6622 1493 Size: 2642 226 152 2109 1697 Fragments arranged by size: 2642 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5380 160 Size: 872 4348 1606 Fragments arranged by size: 4348 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 AvaI C'yCGr_G Cuts at: 1870 1943 2547 3400 4151 1870 Size: 73 604 853 751 4545 Fragments arranged by size: 4545 853 751 604 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3241 3274 3241 Size: 33 6793 BamHI G'GATC_C Cuts at: 4156 4523 4156 Size: 367 6459 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2387 4147 5602 618 Size: 325 217 627 600 1760 1455 1842 Fragments arranged by size: 1842 1760 1455 627 600 325 217 BanII G_rGCy'C Cuts at: 1391 3912 4017 4145 1391 Size: 2521 105 128 4072 Fragments arranged by size: 4072 2521 128 105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2429 928 Size: 971 530 5325 Fragments arranged by size: 5325 971 530 BbvCI CC'TCA_GC Cuts at: 2454 3272 2454 Size: 818 6008 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5017 5051 1903 Size: 34 3080 34 3678 Fragments arranged by size: 3678 3080 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4718 5928 4718 Size: 1210 5616 BclI T'GATC_A Cuts at: 1548 1548 Size: 6826 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2725 3698 5437 Size: 107 122 71 631 605 1053 973 1739 Cuts at: 5437 136 Size: 1525 Fragments arranged by size: 1739 1525 1053 973 631 605 122 107 71 BglII A'GATC_T Cuts at: 2685 6821 2685 Size: 4136 2690 BlpI GC'TnA_GC Cuts at: 3078 4243 3078 Size: 1165 5661 Bme1580I G_kGCm'C Cuts at: 1790 2390 2574 4887 6043 1790 Size: 600 184 2313 1156 2573 Fragments arranged by size: 2573 2313 1156 600 184 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6826 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3619 3779 4557 Size: 416 53 83 399 24 2202 160 778 Cuts at: 4557 5515 442 Size: 958 1753 Fragments arranged by size: 2202 1753 958 778 416 399 160 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6826 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2074 2844 2074 Size: 770 6056 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2750 5486 1239 Size: 1511 2736 2579 Fragments arranged by size: 2736 2579 1511 Bpu10I CC'TnA_GC Cuts at: 2454 2508 2703 3272 3990 2454 Size: 54 195 569 718 5290 Fragments arranged by size: 5290 718 569 195 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3223 3896 4085 4951 5816 6114 3223 Size: 673 189 866 865 298 3935 Fragments arranged by size: 3935 866 865 673 298 189 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2945 5489 882 Size: 617 1446 2544 2219 Fragments arranged by size: 2544 2219 1446 617 BsaAI yAC'GTr Cuts at: 493 1204 2878 4080 4123 4617 493 Size: 711 1674 1202 43 494 2702 Fragments arranged by size: 2702 1674 1202 711 494 43 BsaBI GATnn'nnATC Cuts at: 2930 6820 2930 Size: 3890 2936 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4633 5015 5650 275 Size: 53 83 186 936 3100 382 635 1451 Fragments arranged by size: 3100 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3171 5259 5657 5931 6078 6489 3171 Size: 2088 398 274 147 411 3508 Fragments arranged by size: 3508 2088 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2942 2972 3999 4029 1178 Size: 30 165 30 1539 30 1027 30 3975 Fragments arranged by size: 3975 1539 1027 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3720 3723 1186 Size: 2534 3 4289 Fragments arranged by size: 4289 2534 3 BseYI C'CCAG_C Cuts at: 2815 3109 3113 3126 3790 3905 6029 6525 2815 Size: 294 4 13 664 115 2124 496 3116 Fragments arranged by size: 3116 2124 664 496 294 115 13 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2780 2809 3311 3968 1163 Size: 573 1044 29 502 657 4021 Fragments arranged by size: 4021 1044 657 573 502 29 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2485 4276 5037 6065 1540 Size: 275 186 484 1791 761 1028 2301 Fragments arranged by size: 2301 1791 1028 761 484 275 186 BsiHKAI G_wGCw'C Cuts at: 2574 2704 4017 4887 4972 6043 2574 Size: 130 1313 870 85 1071 3357 Fragments arranged by size: 3357 1313 1071 870 130 85 BsmI GAATG_Cn' Cuts at: 4337 4430 4337 Size: 93 6733 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2390 2574 2704 3912 4017 4145 4887 Size: 399 600 184 130 1208 105 128 742 Cuts at: 4887 4972 6043 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1208 1071 742 600 399 184 130 128 105 85 BspEI T'CCGG_A Cuts at: 3171 6489 3171 Size: 3318 3508 BspHI T'CATG_A Cuts at: 2646 4715 6445 2646 Size: 2069 1730 3027 Fragments arranged by size: 3027 2069 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2464 3124 4181 844 Size: 1620 660 1057 3489 Fragments arranged by size: 3489 1620 1057 660 BsrBI CCG'CTC Cuts at: 1394 1823 2743 3207 4042 4713 1394 Size: 429 920 464 835 671 3507 Fragments arranged by size: 3507 920 835 671 464 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3666 5321 5495 65 Size: 1183 2418 1655 174 1396 Fragments arranged by size: 2418 1655 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5470 5657 1700 Size: 110 3660 187 2869 Fragments arranged by size: 3660 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6826 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6826 BssSI C'ACGA_G Cuts at: 4886 5898 4886 Size: 1012 5814 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6826 BstBI TT'CG_AA Cuts at: 2007 5630 2007 Size: 3623 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6826 BstXI CCAn_nnnn'nTGG Cuts at: 3632 3632 Size: 6826 BstYI r'GATC_y Cuts at: 1972 2685 3498 3966 4156 4523 4908 4925 6377 Size: 713 813 468 190 367 385 17 1452 Cuts at: 6377 6463 6475 6821 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 813 713 468 385 367 346 190 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2940 4049 2940 Size: 1109 5717 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6274 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2856 2889 487 Size: 683 56 1630 33 4424 Fragments arranged by size: 4424 1630 683 56 33 BtsI GCAGTG_nn' Cuts at: 2538 3924 4342 5136 5156 2538 Size: 1386 418 794 20 4208 Fragments arranged by size: 4208 1386 794 418 20 ClaI AT'CG_AT Cuts at: 1554 3597 1554 Size: 2043 4783 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3492 3527 529 Size: 35 2928 35 3828 Fragments arranged by size: 3828 2928 35 35 DraI TTT'AAA Cuts at: 4130 4130 Size: 6826 DraIII CAC_nnn'GTG Cuts at: 2578 2881 3627 2578 Size: 303 746 5777 Fragments arranged by size: 5777 746 303 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3167 4082 5833 809 Size: 507 1851 915 1751 1802 Fragments arranged by size: 1851 1802 1751 915 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2461 2580 2748 Size: 54 1425 50 275 18 631 119 168 Cuts at: 2748 2997 3618 4273 5162 6791 8 Size: 249 621 655 889 1629 43 Fragments arranged by size: 1629 1425 889 655 631 621 275 249 168 119 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4273 1537 Size: 275 2461 4090 Fragments arranged by size: 4090 2461 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2412 4756 1353 Size: 1059 2344 3423 Fragments arranged by size: 3423 2344 1059 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3774 5413 5787 5933 387 Size: 1064 138 2185 1639 374 146 1280 Fragments arranged by size: 2185 1639 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4015 4015 Size: 6826 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2543 2666 2750 2995 3332 3864 4889 Size: 393 911 123 84 245 337 532 1025 Cuts at: 4889 5486 6273 6541 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1025 911 787 597 532 393 337 268 245 123 84 EcoNI CCTnn'n_nnAGG Cuts at: 2507 2507 Size: 6826 EcoO109I rG'GnC_Cy Cuts at: 3136 3385 3760 3136 Size: 249 375 6202 Fragments arranged by size: 6202 375 249 EcoRI G'AATT_C Cuts at: 4135 4513 4135 Size: 378 6448 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3268 3268 Size: 6826 FspI TGC'GCA Cuts at: 5332 5332 Size: 6826 HaeII r_GCGC'y Cuts at: 1678 5973 1678 Size: 4295 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5467 5499 5541 5573 1373 Size: 32 135 32 3895 32 42 32 2626 Fragments arranged by size: 3895 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2065 3260 3751 4170 4416 669 Size: 988 281 127 1195 491 419 246 3079 Fragments arranged by size: 3079 1195 988 491 419 281 246 127 HindIII A'AGCT_T Cuts at: 748 2103 748 Size: 1355 5471 HpaI GTT'AAC Cuts at: 4416 4416 Size: 6826 KpnI G_GTAC'C Cuts at: 4151 4151 Size: 6826 MfeI C'AATT_G Cuts at: 4425 4425 Size: 6826 MluI A'CGCG_T Cuts at: 6702 6702 Size: 6826 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2358 2583 3591 5940 6124 1305 Size: 1053 225 1008 2349 184 2007 Fragments arranged by size: 2349 2007 1053 1008 225 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2582 3620 10 Size: 54 1425 343 750 1038 3216 Fragments arranged by size: 3216 1425 1038 750 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4784 5143 5302 518 Size: 648 3618 359 159 2042 Fragments arranged by size: 3618 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2797 2992 3113 3367 3982 4242 4919 6067 Size: 1191 195 121 254 615 260 677 1148 Cuts at: 6067 6312 1606 Size: 245 2120 Fragments arranged by size: 2120 1191 1148 677 615 260 254 245 195 121 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6274 NdeI CA'TA_TG Cuts at: 387 387 Size: 6826 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6826 NotI GC'GGCC_GC Cuts at: 4273 4273 Size: 6826 NspI r_CATG'y Cuts at: 1132 1229 4184 5729 1132 Size: 97 2955 1545 2229 Fragments arranged by size: 2955 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2166 1348 Size: 818 6008 PciI A'CATG_T Cuts at: 5725 5725 Size: 6826 PflMI CCAn_nnn'nTGG Cuts at: 1108 2602 2669 2881 3627 4036 1108 Size: 1494 67 212 746 409 3898 Fragments arranged by size: 3898 1494 746 409 212 67 PmeI GTTT'AAAC Cuts at: 4130 4130 Size: 6826 PmlI CAC'GTG Cuts at: 2878 2878 Size: 6826 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2603 2635 3956 3988 4895 4927 6408 6440 2603 Size: 32 1321 32 907 32 1481 32 2989 Fragments arranged by size: 2989 1481 1321 907 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3136 3385 3760 3136 Size: 249 375 6202 Fragments arranged by size: 6202 375 249 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6826 PsiI TTA'TAA Cuts at: 4396 4396 Size: 6826 PstI C_TGCA'G Cuts at: 830 2475 3197 3397 4178 830 Size: 1645 722 200 781 3478 Fragments arranged by size: 3478 1645 781 722 200 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6826 PvuII CAG'CTG Cuts at: 2797 2992 3113 3982 2797 Size: 195 121 869 5641 Fragments arranged by size: 5641 869 195 121 RsrII CG'GwC_CG Cuts at: 3174 3174 Size: 6826 SacI G_AGCT'C Cuts at: 4017 4017 Size: 6826 SalI G'TCGA_C Cuts at: 1936 3258 4168 1936 Size: 1322 910 4594 Fragments arranged by size: 4594 1322 910 SbfI CC_TGCA'GG Cuts at: 2475 4178 2475 Size: 1703 5123 ScaI AGT'ACT Cuts at: 5074 6786 5074 Size: 1712 5114 SexAI A'CCwGG_T Cuts at: 2595 2595 Size: 6826 SfcI C'TryA_G Cuts at: 826 1045 2471 3193 3393 3815 4174 5309 5990 Size: 219 1426 722 200 422 359 1135 681 Cuts at: 5990 6181 826 Size: 191 1471 Fragments arranged by size: 1471 1426 1135 722 681 422 359 219 200 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6826 SmaI CCC'GGG Cuts at: 1872 2549 3402 4153 1872 Size: 677 853 751 4545 Fragments arranged by size: 4545 853 751 677 SmlI C'TyrA_G Cuts at: 820 1017 1943 3202 3911 4100 4930 5831 6093 Size: 197 926 1259 709 189 830 901 262 Cuts at: 6093 820 Size: 1553 Fragments arranged by size: 1553 1259 926 901 830 709 262 197 189 SnaBI TAC'GTA Cuts at: 493 4123 4617 493 Size: 3630 494 2702 Fragments arranged by size: 3630 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6826 SphI G_CATG'C Cuts at: 1132 4184 1132 Size: 3052 3774 SspI AAT'ATT Cuts at: 5 52 4750 5 Size: 47 4698 2081 Fragments arranged by size: 4698 2081 47 StuI AGG'CCT Cuts at: 1517 3525 1517 Size: 2008 4818 StyI C'CwwG_G Cuts at: 513 1065 4265 513 Size: 552 3200 3074 Fragments arranged by size: 3200 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2178 2969 3140 3143 3638 3666 4853 Size: 1255 598 791 171 3 495 28 1187 Cuts at: 4853 4870 5023 5208 5653 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1187 791 598 495 445 185 171 153 28 17 3 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5072 6784 96 Size: 274 80 33 51 1443 3095 1712 138 Fragments arranged by size: 3095 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2942 2974 3999 4031 6786 6818 2942 Size: 32 1025 32 2755 32 2950 Fragments arranged by size: 2950 2755 1025 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3215 1316 Size: 1899 4927 XbaI T'CTAG_A Cuts at: 4162 4162 Size: 6826 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3613 3671 3613 Size: 58 6768 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6826 XmaI C'CCGG_G Cuts at: 1870 2547 3400 4151 1870 Size: 677 853 751 4545 Fragments arranged by size: 4545 853 751 677 XmnI GAAnn'nnTTC Cuts at: 2081 2105 4955 2081 Size: 24 2850 3952 Fragments arranged by size: 3952 2850 24 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4634 276 Size: 53 83 186 936 3100 2468 Fragments arranged by size: 3100 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI SacII SanDI SapI SfiI SfoI SrfI SwaI