NCSTN ORF clone pFN21ASDA0253

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Halotag ORF Clone pFN21ASDA0253

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Clone info.

Species

human

Clone Name

pFN21ASDA0253 Sequence (with vector) Restriction Map

Product ID

FHC00480

Flexi Clone PID

FXC00480

ORF Sequence

DNA sequence (2127 bp)
Protein sequence (709 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383822]

Gene

KIAA0253

Symbol

NCSTN

Alias : ATAG1874

Description

nicastrin, transcript variant 1

Original Clone ID

ha07036

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2127 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 709 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1203.1 0 100.0 NCSTN CCDS76228.1 0 99.0 NCSTN The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR008710 274 499 PF05450 Nicastrin
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_056146 709 142690,605254 100.0 100.0 NP_001277113 689 142690,605254 99.0 97.2 XP_011507665 638 142690,605254 90.0 100.0 NP_001277115 571 142690,605254 80.5 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0253 Download

>KIAA0253  2127 bp
ATGGCTACGGCAGGGGGTGGCTCTGGGGCTGACCCGGGAAGTCGGGGTCTCCTTCGCCTT
CTGTCTTTCTGCGTCCTACTAGCAGGTTTGTGCAGGGGAAACTCAGTGGAGAGGAAGATA
TATATCCCCTTAAATAAAACAGCTCCCTGTGTTCGCCTGCTCAACGCCACTCATCAGATT
GGCTGCCAGTCTTCAATTAGTGGAGACACAGGGGTTATCCACGTAGTAGAGAAAGAGGAG
GACCTACAGTGGGTATTGACTGATGGCCCCAACCCCCCTTACATGGTTCTGCTGGAGAGC
AAGCATTTTACCAGGGATTTAATGGAGAAGCTGAAAGGGAGAACCAGCCGAATTGCTGGT
CTTGCAGTGTCCTTGACCAAGCCCAGTCCTGCCTCAGGCTTCTCTCCTAGTGTACAGTGC
CCAAATGATGGGTTTGGTGTTTACTCCAATTCCTATGGGCCAGAGTTTGCTCACTGCAGA
GAAATACAGTGGAATTCGCTGGGCAATGGTTTGGCTTATGAAGACTTTAGTTTCCCCATC
TTTCTTCTTGAAGATGAAAATGAAACCAAAGTCATCAAGCAGTGCTATCAAGATCACAAC
CTGAGTCAGAATGGCTCAGCACCAACCTTCCCACTATGTGCCATGCAGCTCTTTTCACAC
ATGCATGCTGTCATCAGCACTGCCACCTGCATGCGGCGCAGCTCCATCCAAAGCACCTTC
AGCATCAACCCAGAAATCGTCTGTGACCCCCTGTCTGATTACAATGTGTGGAGCATGCTA
AAGCCTATAAATACAACTGGGACATTAAAGCCTGACGACAGGGTTGTGGTTGCTGCCACC
CGGCTGGATAGTCGTTCCTTTTTCTGGAATGTGGCCCCAGGGGCTGAAAGCGCAGTGGCT
TCCTTTGTCACCCAGCTGGCTGCTGCTGAAGCTTTGCAAAAGGCACCTGATGTGACCACC
CTGCCCCGCAATGTCATGTTTGTCTTCTTTCAAGGGGAAACTTTTGACTACATTGGCAGC
TCGAGGATGGTCTACGATATGGAGAAGGGCAAGTTTCCCGTGCAGTTAGAGAATGTTGAC
TCATTTGTGGAGCTGGGACAGGTGGCCTTAAGAACTTCATTAGAGCTTTGGATGCACACA
GATCCTGTTTCTCAGAAAAATGAGTCTGTACGGAACCAGGTGGAGGATCTCCTGGCCACA
TTGGAGAAGAGTGGTGCTGGTGTCCCTGCTGTCATCCTCAGGAGGCCAAATCAGTCCCAG
CCTCTCCCACCATCTTCCCTGCAGCGATTTCTTCGAGCTCGAAACATCTCTGGCGTTGTT
CTGGCTGACCACTCTGGTGCCTTCCATAACAAATATTACCAGAGTATTTACGACACTGCT
GAGAACATTAATGTGAGCTATCCCGAATGGCTGAGCCCTGAAGAGGACCTGAACTTTGTA
ACAGACACTGCCAAGGCCCTGGCAGATGTGGCCACGGTGCTGGGACGTGCTCTGTATGAG
CTTGCAGGAGGAACCAACTTCAGCGACACAGTTCAGGCTGATCCCCAAACGGTTACCCGC
CTGCTCTATGGGTTCCTGATTAAAGCCAACAACTCATGGTTCCAGTCTATCCTCAGGCAG
GACCTAAGGTCCTACTTGGGTGACGGGCCTCTTCAACATTACATCGCTGTCTCCAGCCCC
ACCAACACCACTTATGTTGTACAGTATGCCTTGGCAAATTTGACTGGCACAGTGGTCAAC
CTCACCCGAGAGCAGTGCCAGGATCCAAGTAAAGTCCCAAGTGAAAACAAGGATCTGTAT
GAGTACTCATGGGTCCAGGGCCCTTTGCATTCTAATGAGACGGACCGACTCCCCCGGTGT
GTGCGTTCTACTGCACGATTAGCCAGGGCCTTGTCTCCTGCCTTTGAACTGAGTCAGTGG
AGCTCTACTGAATACTCTACATGGACTGAGAGCCGCTGGAAAGATATCCGTGCCCGGATA
TTTCTCATCGCCAGCAAAGAGCTTGAGTTGATCACCCTGACAGTGGGCTTCGGCATCCTC
ATCTTCTCCCTCATCGTCACCTACTGCATCAATGCCAAAGCTGATGTCCTTTTCATTGCT
CCCCGGGAGCCAGGAGCTGTGTCATAC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0253 Download

>KIAA0253  709 aa
MATAGGGSGADPGSRGLLRLLSFCVLLAGLCRGNSVERKIYIPLNKTAPCVRLLNATHQI
GCQSSISGDTGVIHVVEKEEDLQWVLTDGPNPPYMVLLESKHFTRDLMEKLKGRTSRIAG
LAVSLTKPSPASGFSPSVQCPNDGFGVYSNSYGPEFAHCREIQWNSLGNGLAYEDFSFPI
FLLEDENETKVIKQCYQDHNLSQNGSAPTFPLCAMQLFSHMHAVISTATCMRRSSIQSTF
SINPEIVCDPLSDYNVWSMLKPINTTGTLKPDDRVVVAATRLDSRSFFWNVAPGAESAVA
SFVTQLAAAEALQKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMEKGKFPVQLENVD
SFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLATLEKSGAGVPAVILRRPNQSQ
PLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFV
TDTAKALADVATVLGRALYELAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQ
DLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLY
EYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIRARI
FLIASKELELITLTVGFGILIFSLIVTYCINAKADVLFIAPREPGAVSY

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0253 Download

>pFN21ASDA0253  6856 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCTACGGCAGGGGG
TGGCTCTGGGGCTGACCCGGGAAGTCGGGGTCTCCTTCGCCTTCTGTCTTTCTGCGTCCT
ACTAGCAGGTTTGTGCAGGGGAAACTCAGTGGAGAGGAAGATATATATCCCCTTAAATAA
AACAGCTCCCTGTGTTCGCCTGCTCAACGCCACTCATCAGATTGGCTGCCAGTCTTCAAT
TAGTGGAGACACAGGGGTTATCCACGTAGTAGAGAAAGAGGAGGACCTACAGTGGGTATT
GACTGATGGCCCCAACCCCCCTTACATGGTTCTGCTGGAGAGCAAGCATTTTACCAGGGA
TTTAATGGAGAAGCTGAAAGGGAGAACCAGCCGAATTGCTGGTCTTGCAGTGTCCTTGAC
CAAGCCCAGTCCTGCCTCAGGCTTCTCTCCTAGTGTACAGTGCCCAAATGATGGGTTTGG
TGTTTACTCCAATTCCTATGGGCCAGAGTTTGCTCACTGCAGAGAAATACAGTGGAATTC
GCTGGGCAATGGTTTGGCTTATGAAGACTTTAGTTTCCCCATCTTTCTTCTTGAAGATGA
AAATGAAACCAAAGTCATCAAGCAGTGCTATCAAGATCACAACCTGAGTCAGAATGGCTC
AGCACCAACCTTCCCACTATGTGCCATGCAGCTCTTTTCACACATGCATGCTGTCATCAG
CACTGCCACCTGCATGCGGCGCAGCTCCATCCAAAGCACCTTCAGCATCAACCCAGAAAT
CGTCTGTGACCCCCTGTCTGATTACAATGTGTGGAGCATGCTAAAGCCTATAAATACAAC
TGGGACATTAAAGCCTGACGACAGGGTTGTGGTTGCTGCCACCCGGCTGGATAGTCGTTC
CTTTTTCTGGAATGTGGCCCCAGGGGCTGAAAGCGCAGTGGCTTCCTTTGTCACCCAGCT
GGCTGCTGCTGAAGCTTTGCAAAAGGCACCTGATGTGACCACCCTGCCCCGCAATGTCAT
GTTTGTCTTCTTTCAAGGGGAAACTTTTGACTACATTGGCAGCTCGAGGATGGTCTACGA
TATGGAGAAGGGCAAGTTTCCCGTGCAGTTAGAGAATGTTGACTCATTTGTGGAGCTGGG
ACAGGTGGCCTTAAGAACTTCATTAGAGCTTTGGATGCACACAGATCCTGTTTCTCAGAA
AAATGAGTCTGTACGGAACCAGGTGGAGGATCTCCTGGCCACATTGGAGAAGAGTGGTGC
TGGTGTCCCTGCTGTCATCCTCAGGAGGCCAAATCAGTCCCAGCCTCTCCCACCATCTTC
CCTGCAGCGATTTCTTCGAGCTCGAAACATCTCTGGCGTTGTTCTGGCTGACCACTCTGG
TGCCTTCCATAACAAATATTACCAGAGTATTTACGACACTGCTGAGAACATTAATGTGAG
CTATCCCGAATGGCTGAGCCCTGAAGAGGACCTGAACTTTGTAACAGACACTGCCAAGGC
CCTGGCAGATGTGGCCACGGTGCTGGGACGTGCTCTGTATGAGCTTGCAGGAGGAACCAA
CTTCAGCGACACAGTTCAGGCTGATCCCCAAACGGTTACCCGCCTGCTCTATGGGTTCCT
GATTAAAGCCAACAACTCATGGTTCCAGTCTATCCTCAGGCAGGACCTAAGGTCCTACTT
GGGTGACGGGCCTCTTCAACATTACATCGCTGTCTCCAGCCCCACCAACACCACTTATGT
TGTACAGTATGCCTTGGCAAATTTGACTGGCACAGTGGTCAACCTCACCCGAGAGCAGTG
CCAGGATCCAAGTAAAGTCCCAAGTGAAAACAAGGATCTGTATGAGTACTCATGGGTCCA
GGGCCCTTTGCATTCTAATGAGACGGACCGACTCCCCCGGTGTGTGCGTTCTACTGCACG
ATTAGCCAGGGCCTTGTCTCCTGCCTTTGAACTGAGTCAGTGGAGCTCTACTGAATACTC
TACATGGACTGAGAGCCGCTGGAAAGATATCCGTGCCCGGATATTTCTCATCGCCAGCAA
AGAGCTTGAGTTGATCACCCTGACAGTGGGCTTCGGCATCCTCATCTTCTCCCTCATCGT
CACCTACTGCATCAATGCCAAAGCTGATGTCCTTTTCATTGCTCCCCGGGAGCCAGGAGC
TGTGTCATACTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT