(Circular) (Six-base) MAPSORT of: pFN21ASDA0253.seq Check: 5451 from: 1 to: 6856 pFN21ASDA0253 6856 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2717 2717 Size: 6856 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4666 278 Size: 53 83 186 936 3130 2468 Fragments arranged by size: 3130 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3054 4199 1937 Size: 1117 1145 4594 Fragments arranged by size: 4594 1145 1117 Acc65I G'GTAC_C Cuts at: 4177 4177 Size: 6856 AclI AA'CG_TT Cuts at: 1563 4983 5356 1563 Size: 3420 373 3063 Fragments arranged by size: 3420 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2725 2970 3462 3526 4919 6303 6571 1632 Size: 1093 245 492 64 1393 1384 268 1917 Fragments arranged by size: 1917 1393 1384 1093 492 268 245 64 AflII C'TTAA_G Cuts at: 820 1017 3130 820 Size: 197 2113 4546 Fragments arranged by size: 4546 2113 197 AflIII A'CryG_T Cuts at: 1203 5755 6732 1203 Size: 4552 977 1327 Fragments arranged by size: 4552 1327 977 AgeI A'CCGG_T Cuts at: 5687 5687 Size: 6856 AhdI GACnn_n'nnGTC Cuts at: 2773 3649 5585 2773 Size: 876 1936 4044 Fragments arranged by size: 4044 1936 876 AleI CACnn'nnGTG Cuts at: 1166 3357 1166 Size: 2191 4665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2128 1184 Size: 944 5912 AlwNI CAG_nnn'CTG Cuts at: 2906 3167 3470 4139 4599 6171 2906 Size: 261 303 669 460 1572 3591 Fragments arranged by size: 3591 1572 669 460 303 261 ApaI G_GGCC'C Cuts at: 3845 3845 Size: 6856 ApaLI G'TGCA_C Cuts at: 4913 6069 4913 Size: 1156 5700 ApoI r'AATT_y Cuts at: 1493 2515 3739 4165 4391 4543 6652 1493 Size: 1022 1224 426 226 152 2109 1697 Fragments arranged by size: 2109 1697 1224 1022 426 226 152 AseI AT'TA_AT Cuts at: 160 1032 3411 5410 160 Size: 872 2379 1999 1606 Fragments arranged by size: 2379 1999 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 AvaI C'yCGr_G Cuts at: 1870 1943 2056 3043 3768 4125 4181 1870 Size: 73 113 987 725 357 56 4545 Fragments arranged by size: 4545 987 725 357 113 73 56 BamHI G'GATC_C Cuts at: 3784 4186 4553 3784 Size: 402 367 6087 Fragments arranged by size: 6087 402 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2965 3359 4177 5632 618 Size: 325 217 627 1178 394 818 1455 1842 Fragments arranged by size: 1842 1455 1178 818 627 394 325 217 BanII G_rGCy'C Cuts at: 1391 3322 3440 3845 3947 4175 1391 Size: 1931 118 405 102 228 4072 Fragments arranged by size: 4072 1931 405 228 118 102 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2205 2550 2998 928 Size: 971 306 345 448 4786 Fragments arranged by size: 4786 971 448 345 306 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3759 3793 5047 5081 1903 Size: 34 1822 34 1254 34 3678 Fragments arranged by size: 3678 1822 1254 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4748 5958 4748 Size: 1210 5646 BclI T'GATC_A Cuts at: 1548 4032 1548 Size: 2484 4372 BfrBI ATG'CAT Cuts at: 2686 2686 Size: 6856 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2903 5467 136 Size: 107 122 71 631 605 1231 2564 1525 Fragments arranged by size: 2564 1525 1231 631 605 122 107 71 BglII A'GATC_T Cuts at: 6851 6851 Size: 6856 BlpI GC'TnA_GC Cuts at: 2638 3434 4273 2638 Size: 796 839 5221 Fragments arranged by size: 5221 839 796 Bme1580I G_kGCm'C Cuts at: 1790 2444 3845 3997 4917 6073 1790 Size: 654 1401 152 920 1156 2573 Fragments arranged by size: 2573 1401 1156 920 654 152 BmgBI CAC'GTC Cuts at: 1318 3509 1318 Size: 2191 4665 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2400 2829 4587 Size: 416 53 83 399 24 983 429 1758 Cuts at: 4587 5545 442 Size: 958 1753 Fragments arranged by size: 1758 1753 983 958 429 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6856 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 3679 5516 1239 Size: 1097 1343 1837 2579 Fragments arranged by size: 2579 1837 1343 1097 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4046 4981 5846 6144 4046 Size: 935 865 298 4758 Fragments arranged by size: 4758 935 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2075 5519 882 Size: 617 576 3444 2219 Fragments arranged by size: 3444 2219 617 576 BsaAI yAC'GTr Cuts at: 493 1204 2245 4153 4647 493 Size: 711 1041 1908 494 2702 Fragments arranged by size: 2702 1908 1041 711 494 BsaBI GATnn'nnATC Cuts at: 2144 6850 2144 Size: 4706 2150 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4663 5045 5680 275 Size: 53 83 186 936 3130 382 635 1451 Fragments arranged by size: 3130 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5289 5687 5961 6108 6519 5289 Size: 398 274 147 411 5626 Fragments arranged by size: 5626 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6631 Fragments arranged by size: 6631 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2273 1186 Size: 1087 5769 BseYI C'CCAG_C Cuts at: 2521 2934 3115 3279 3502 6059 6555 2521 Size: 413 181 164 223 2557 496 2822 Fragments arranged by size: 2822 2557 496 413 223 181 164 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2134 3104 3879 1163 Size: 573 398 970 775 4140 Fragments arranged by size: 4140 970 775 573 398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4306 5067 6095 1540 Size: 275 186 2305 761 1028 2301 Fragments arranged by size: 2305 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3322 3514 3947 4917 5002 6073 3322 Size: 192 433 970 85 1071 4105 Fragments arranged by size: 4105 1071 970 433 192 85 BsmI GAATG_Cn' Cuts at: 3850 4367 4460 3850 Size: 517 93 6246 Fragments arranged by size: 6246 517 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3854 3854 Size: 6856 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2444 3322 3440 3514 3845 3947 3997 Size: 399 654 878 118 74 331 102 50 Cuts at: 3997 4175 4917 5002 6073 1391 Size: 178 742 85 1071 2174 Fragments arranged by size: 2174 1071 878 742 654 399 331 178 118 102 85 74 50 BspEI T'CCGG_A Cuts at: 6519 6519 Size: 6856 BspHI T'CATG_A Cuts at: 4745 6475 4745 Size: 1730 5126 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2096 2717 4211 844 Size: 1252 621 1494 3489 Fragments arranged by size: 3489 1494 1252 621 BsrBI CCG'CTC Cuts at: 1394 1823 4743 1394 Size: 429 2920 3507 Fragments arranged by size: 3507 2920 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2533 2998 4116 5351 5525 65 Size: 1183 1285 465 1118 1235 174 1396 Fragments arranged by size: 1396 1285 1235 1183 1118 465 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5500 5687 1700 Size: 110 3690 187 2869 Fragments arranged by size: 3690 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2434 3721 96 Size: 2338 1287 3231 Fragments arranged by size: 3231 2338 1287 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6856 BssSI C'ACGA_G Cuts at: 4916 5928 4916 Size: 1012 5844 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6856 BstBI TT'CG_AA Cuts at: 2007 5660 2007 Size: 3653 3203 BstEII G'GTnAC_C Cuts at: 1184 3574 1184 Size: 2390 4466 BstXI CCAn_nnnn'nTGG Cuts at: 2866 3481 3502 2866 Size: 615 21 6220 Fragments arranged by size: 6220 615 21 BstYI r'GATC_y Cuts at: 1972 3163 3208 3784 3814 4186 4553 4938 4955 Size: 1191 45 576 30 372 367 385 17 Cuts at: 4955 6407 6493 6505 6851 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1191 576 385 372 367 346 86 45 30 17 12 Bsu36I CC'TnA_GG Cuts at: 2416 3260 3635 3647 2416 Size: 844 375 12 5625 Fragments arranged by size: 5625 844 375 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3495 513 Size: 552 957 1473 3874 Fragments arranged by size: 3874 1473 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3670 3994 487 Size: 683 56 2444 324 3349 Fragments arranged by size: 3349 2444 683 324 56 BtsI GCAGTG_nn' Cuts at: 2394 2494 2609 2700 2922 3396 3468 3782 4372 Size: 100 115 91 222 474 72 314 590 Cuts at: 4372 5166 5186 2394 Size: 794 20 4064 Fragments arranged by size: 4064 794 590 474 314 222 115 100 91 72 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6856 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3700 3735 529 Size: 35 3136 35 3650 Fragments arranged by size: 3650 3136 35 35 DraI TTT'AAA Cuts at: 4160 4160 Size: 6856 DraIII CAC_nnn'GTG Cuts at: 2660 2660 Size: 6856 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5863 809 Size: 507 4547 1802 Fragments arranged by size: 4547 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3216 3492 4303 Size: 54 1425 50 275 18 1386 276 811 Cuts at: 4303 5192 6821 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1386 889 811 276 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4303 1537 Size: 275 2491 4090 Fragments arranged by size: 4090 2491 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3224 3438 3678 4786 1353 Size: 1871 214 240 1108 3423 Fragments arranged by size: 3423 1871 1108 240 214 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5443 5817 5963 387 Size: 1064 138 3854 374 146 1280 Fragments arranged by size: 3854 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3320 3945 3320 Size: 625 6231 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2725 2970 3462 3526 3679 4919 Size: 393 704 389 245 492 64 153 1240 Cuts at: 4919 5516 6303 6571 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1240 787 704 597 492 393 389 268 245 153 64 EcoNI CCTnn'n_nnAGG Cuts at: 2415 3638 2415 Size: 1223 5633 EcoO109I rG'GnC_Cy Cuts at: 2263 3448 3478 3643 3651 3841 3842 3909 2263 Size: 1185 30 165 8 190 1 67 5210 Fragments arranged by size: 5210 1185 190 165 67 30 8 1 EcoRI G'AATT_C Cuts at: 2515 4165 4543 2515 Size: 1650 378 4828 Fragments arranged by size: 4828 1650 378 EcoRV GAT'ATC Cuts at: 3988 3988 Size: 6856 FspI TGC'GCA Cuts at: 5362 5362 Size: 6856 HaeII r_GCGC'y Cuts at: 1678 6003 1678 Size: 4325 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2759 2791 3256 3288 3635 Size: 32 135 32 1187 32 465 32 347 Cuts at: 3635 3667 5497 5529 5571 5603 1373 Size: 32 1830 32 42 32 2626 Fragments arranged by size: 2626 1830 1187 465 347 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3100 3760 4200 4446 669 Size: 988 281 1162 660 440 246 3079 Fragments arranged by size: 3079 1162 988 660 440 281 246 HindIII A'AGCT_T Cuts at: 748 2952 748 Size: 2204 4652 HpaI GTT'AAC Cuts at: 4446 4446 Size: 6856 KpnI G_GTAC'C Cuts at: 4181 4181 Size: 6856 MfeI C'AATT_G Cuts at: 4455 4455 Size: 6856 MluI A'CGCG_T Cuts at: 6732 6732 Size: 6856 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5970 6154 1305 Size: 4665 184 2007 Fragments arranged by size: 4665 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3218 3494 10 Size: 54 1425 343 1386 276 3372 Fragments arranged by size: 3372 1425 1386 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 2685 2711 3357 3413 3855 4814 5173 Size: 648 1519 26 646 56 442 959 359 Cuts at: 5173 5332 518 Size: 159 2042 Fragments arranged by size: 2042 1519 959 648 646 442 359 159 56 26 MspA1I CmG'CkG Cuts at: 1606 2938 3978 4272 4949 6097 6342 1606 Size: 1332 1040 294 677 1148 245 2120 Fragments arranged by size: 2120 1332 1148 1040 677 294 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5347 Fragments arranged by size: 5347 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6856 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6856 NotI GC'GGCC_GC Cuts at: 4303 4303 Size: 6856 NsiI A_TGCA'T Cuts at: 2688 2688 Size: 6856 NspI r_CATG'y Cuts at: 1132 1229 2686 2690 2716 2800 4214 5759 1132 Size: 97 1457 4 26 84 1414 1545 2229 Fragments arranged by size: 2229 1545 1457 1414 97 84 26 4 PasI CC'CwG_GG Cuts at: 1348 2900 1348 Size: 1552 5304 PciI A'CATG_T Cuts at: 5755 5755 Size: 6856 PflMI CCAn_nnn'nTGG Cuts at: 1108 2450 1108 Size: 1342 5514 PmeI GTTT'AAAC Cuts at: 4160 4160 Size: 6856 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4925 4957 6438 6470 4925 Size: 32 1481 32 5311 Fragments arranged by size: 5311 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2263 3448 3643 3651 2263 Size: 1185 195 8 5468 Fragments arranged by size: 5468 1185 195 8 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6856 PsiI TTA'TAA Cuts at: 4426 4426 Size: 6856 PspOMI G'GGCC_C Cuts at: 3841 3841 Size: 6856 PspXI vC'TCGA_Gb Cuts at: 3043 3043 Size: 6856 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2011 2043 2011 Size: 32 6824 PstI C_TGCA'G Cuts at: 830 2501 3306 4208 830 Size: 1671 805 902 3478 Fragments arranged by size: 3478 1671 902 805 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6856 PvuII CAG'CTG Cuts at: 2938 2938 Size: 6856 RsrII CG'GwC_CG Cuts at: 3865 3865 Size: 6856 SacI G_AGCT'C Cuts at: 3322 3947 3322 Size: 625 6231 SalI G'TCGA_C Cuts at: 1936 4198 1936 Size: 2262 4594 SbfI CC_TGCA'GG Cuts at: 4208 4208 Size: 6856 ScaI AGT'ACT Cuts at: 3827 5104 6816 3827 Size: 1277 1712 3867 Fragments arranged by size: 3867 1712 1277 SexAI A'CCwGG_T Cuts at: 3198 3198 Size: 6856 SfcI C'TryA_G Cuts at: 826 1045 2267 2497 3302 4204 5339 6020 6211 Size: 219 1222 230 805 902 1135 681 191 Cuts at: 6211 826 Size: 1471 Fragments arranged by size: 1471 1222 1135 902 805 681 230 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6856 SmaI CCC'GGG Cuts at: 1872 2058 4127 4183 1872 Size: 186 2069 56 4545 Fragments arranged by size: 4545 2069 186 56 SmlI C'TyrA_G Cuts at: 820 1017 1943 3043 3130 4025 4960 5861 6123 Size: 197 926 1100 87 895 935 901 262 Cuts at: 6123 820 Size: 1553 Fragments arranged by size: 1553 1100 935 926 901 895 262 197 87 SnaBI TAC'GTA Cuts at: 493 4153 4647 493 Size: 3660 494 2702 Fragments arranged by size: 3660 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6856 SphI G_CATG'C Cuts at: 1132 2690 2716 2800 4214 1132 Size: 1558 26 84 1414 3774 Fragments arranged by size: 3774 1558 1414 84 26 SspI AAT'ATT Cuts at: 5 52 3377 4780 5 Size: 47 3325 1403 2081 Fragments arranged by size: 3325 2081 1403 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6856 StyI C'CwwG_G Cuts at: 513 1065 2022 3474 3732 4295 513 Size: 552 957 1452 258 563 3074 Fragments arranged by size: 3074 1452 957 563 552 258 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2948 3650 3882 4883 4900 5053 5238 Size: 1255 1368 702 232 1001 17 153 185 Cuts at: 5238 5683 325 Size: 445 1498 Fragments arranged by size: 1498 1368 1255 1001 702 445 232 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2434 3721 3825 Size: 274 80 33 51 1443 457 1287 104 Cuts at: 3825 5102 6814 96 Size: 1277 1712 138 Fragments arranged by size: 1712 1443 1287 1277 457 274 138 104 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2112 2144 2255 2287 2634 2666 6816 6848 2112 Size: 32 111 32 347 32 4150 32 2120 Fragments arranged by size: 4150 2120 347 111 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6856 XbaI T'CTAG_A Cuts at: 4192 4192 Size: 6856 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2341 2341 Size: 6856 XhoI C'TCGA_G Cuts at: 1943 3043 1943 Size: 1100 5756 XmaI C'CCGG_G Cuts at: 1870 2056 4125 4181 1870 Size: 186 2069 56 4545 Fragments arranged by size: 4545 2069 186 56 XmnI GAAnn'nnTTC Cuts at: 4985 4985 Size: 6856 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4664 276 Size: 53 83 186 936 3130 2468 Fragments arranged by size: 3130 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI SacII SanDI SapI SfiI SfoI SrfI SwaI