POFUT1 ORF clone pFN21ASDA0180

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Halotag ORF Clone pFN21ASDA0180

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Clone info.

Species

human

Clone Name

pFN21ASDA0180 Sequence (with vector) Restriction Map

Product ID

FHC00446

Flexi Clone PID

FXC00446

ORF Sequence

DNA sequence (1164 bp)
Protein sequence (388 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383785]

Gene

KIAA0180

Symbol

POFUT1

Alias : DDD2, FUT12, O-FUT, O-Fuc-T, O-FucT-1, OFUCT1

Description

protein O-fucosyltransferase 1, transcript variant 1

Original Clone ID

ha02567s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1164 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 388 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13198.1 4e-172 100.0 POFUT1 CCDS13199.1 9.9e-80 94.8 POFUT1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019378 35 373 PF10250 GDP-fucose protein O-fucosyltransferase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_056167 388 607491,615327 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0180 Download

>KIAA0180  1164 bp
ATGGGCGCCGCCGCGTGGGCACGGCCGCTGAGCGTGTCTTTCCTGCTGCTGCTTCTGCCG
CTCCCGGGGATGCCTGCGGGCTCCTGGGACCCGGCCGGTTACCTGCTCTACTGCCCCTGC
ATGGGGCGCTTTGGGAACCAGGCCGATCACTTCTTGGGCTCTCTGGCATTTGCAAAGCTG
CTAAACCGTACCTTGGCTGTCCCTCCTTGGATTGAGTACCAGCATCACAAGCCTCCTTTC
ACCAACCTCCATGTGTCCTACCAGAAGTACTTCAAGCTGGAGCCCCTCCAGGCTTACCAT
CGGGTCATCAGCTTGGAGGATTTCATGGAGAAGCTGGCACCCACCCACTGGCCCCCTGAG
AAGCGGGTGGCATACTGCTTTGAGGTGGCAGCCCAGCGAAGCCCAGATAAGAAGACGTGC
CCCATGAAGGAAGGAAACCCCTTTGGCCCATTCTGGGATCAGTTTCATGTGAGTTTCAAC
AAGTCGGAGCTTTTTACAGGCATTTCCTTCAGTGCTTCCTACAGAGAACAATGGAGCCAG
AGATTTTCTCCAAAGGAACATCCGGTGCTTGCCCTGCCAGGAGCCCCAGCCCAGTTCCCC
GTCCTAGAGGAACACAGGCCACTACAGAAGTACATGGTATGGTCAGACGAAATGGTGAAG
ACGGGAGAGGCCCAGATTCATGCCCACCTTGTCCGGCCCTATGTGGGCATTCATCTGCGC
ATTGGCTCTGACTGGAAGAACGCCTGTGCCATGCTGAAGGACGGGACTGCAGGCTCGCAC
TTCATGGCCTCTCCGCAGTGTGTGGGCTACAGCCGCAGCACAGCGGCCCCCCTCACGATG
ACTATGTGCCTGCCTGACCTGAAGGAGATCCAGAGGGCTGTGAAGCTCTGGGTGAGGTCG
CTGGATGCCCAGTCGGTCTACGTTGCTACTGATTCCGAGAGTTATGTGCCTGAGCTCCAA
CAGCTCTTCAAAGGGAAGGTGAAGGTGGTGAGCCTGAAGCCTGAGGTGGCCCAGGTCGAC
CTGTACATCCTCGGCCAAGCCGACCACTTTATTGGCAACTGTGTCTCCTCCTTCACTGCC
TTTGTGAAGCGGGAGCGGGACCTCCAGGGGAGGCCGTCTTCTTTCTTCGGCATGGACAGG
CCCCCTAAGCTGCGGGACGAGTTC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0180 Download

>KIAA0180  388 aa
MGAAAWARPLSVSFLLLLLPLPGMPAGSWDPAGYLLYCPCMGRFGNQADHFLGSLAFAKL
LNRTLAVPPWIEYQHHKPPFTNLHVSYQKYFKLEPLQAYHRVISLEDFMEKLAPTHWPPE
KRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYREQWSQ
RFSPKEHPVLALPGAPAQFPVLEEHRPLQKYMVWSDEMVKTGEAQIHAHLVRPYVGIHLR
IGSDWKNACAMLKDGTAGSHFMASPQCVGYSRSTAAPLTMTMCLPDLKEIQRAVKLWVRS
LDAQSVYVATDSESYVPELQQLFKGKVKVVSLKPEVAQVDLYILGQADHFIGNCVSSFTA
FVKRERDLQGRPSSFFGMDRPPKLRDEF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0180 Download

>pFN21ASDA0180  5893 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGGCGCCGCCGCGTG
GGCACGGCCGCTGAGCGTGTCTTTCCTGCTGCTGCTTCTGCCGCTCCCGGGGATGCCTGC
GGGCTCCTGGGACCCGGCCGGTTACCTGCTCTACTGCCCCTGCATGGGGCGCTTTGGGAA
CCAGGCCGATCACTTCTTGGGCTCTCTGGCATTTGCAAAGCTGCTAAACCGTACCTTGGC
TGTCCCTCCTTGGATTGAGTACCAGCATCACAAGCCTCCTTTCACCAACCTCCATGTGTC
CTACCAGAAGTACTTCAAGCTGGAGCCCCTCCAGGCTTACCATCGGGTCATCAGCTTGGA
GGATTTCATGGAGAAGCTGGCACCCACCCACTGGCCCCCTGAGAAGCGGGTGGCATACTG
CTTTGAGGTGGCAGCCCAGCGAAGCCCAGATAAGAAGACGTGCCCCATGAAGGAAGGAAA
CCCCTTTGGCCCATTCTGGGATCAGTTTCATGTGAGTTTCAACAAGTCGGAGCTTTTTAC
AGGCATTTCCTTCAGTGCTTCCTACAGAGAACAATGGAGCCAGAGATTTTCTCCAAAGGA
ACATCCGGTGCTTGCCCTGCCAGGAGCCCCAGCCCAGTTCCCCGTCCTAGAGGAACACAG
GCCACTACAGAAGTACATGGTATGGTCAGACGAAATGGTGAAGACGGGAGAGGCCCAGAT
TCATGCCCACCTTGTCCGGCCCTATGTGGGCATTCATCTGCGCATTGGCTCTGACTGGAA
GAACGCCTGTGCCATGCTGAAGGACGGGACTGCAGGCTCGCACTTCATGGCCTCTCCGCA
GTGTGTGGGCTACAGCCGCAGCACAGCGGCCCCCCTCACGATGACTATGTGCCTGCCTGA
CCTGAAGGAGATCCAGAGGGCTGTGAAGCTCTGGGTGAGGTCGCTGGATGCCCAGTCGGT
CTACGTTGCTACTGATTCCGAGAGTTATGTGCCTGAGCTCCAACAGCTCTTCAAAGGGAA
GGTGAAGGTGGTGAGCCTGAAGCCTGAGGTGGCCCAGGTCGACCTGTACATCCTCGGCCA
AGCCGACCACTTTATTGGCAACTGTGTCTCCTCCTTCACTGCCTTTGTGAAGCGGGAGCG
GGACCTCCAGGGGAGGCCGTCTTCTTTCTTCGGCATGGACAGGCCCCCTAAGCTGCGGGA
CGAGTTCTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT