(Circular) (Six-base) MAPSORT of: pFN21ASDA0180.seq Check: 1602 from: 1 to: 5893 pFN21ASDA0180 5893 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3703 278 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2940 3039 3236 1937 Size: 1003 99 197 4594 Fragments arranged by size: 4594 1003 197 99 Acc65I G'GTAC_C Cuts at: 3214 3214 Size: 5893 AclI AA'CG_TT Cuts at: 1563 4020 4393 1563 Size: 2457 373 3063 Fragments arranged by size: 3063 2457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2515 2798 2903 3038 3956 5340 5608 1632 Size: 883 283 105 135 918 1384 268 1917 Fragments arranged by size: 1917 1384 918 883 283 268 135 105 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5696 AflIII A'CryG_T Cuts at: 1203 4792 5769 1203 Size: 3589 977 1327 Fragments arranged by size: 3589 1327 977 AgeI A'CCGG_T Cuts at: 4724 4724 Size: 5893 AhdI GACnn_n'nnGTC Cuts at: 4622 4622 Size: 5893 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5893 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2753 1184 Size: 1569 4324 AlwNI CAG_nnn'CTG Cuts at: 3636 5208 3636 Size: 1572 4321 ApaLI G'TGCA_C Cuts at: 3950 5106 3950 Size: 1156 4737 ApoI r'AATT_y Cuts at: 1493 3202 3428 3580 5689 1493 Size: 1709 226 152 2109 1697 Fragments arranged by size: 2109 1709 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4447 160 Size: 872 3415 1606 Fragments arranged by size: 3415 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 AvaI C'yCGr_G Cuts at: 1870 1943 2086 3218 1870 Size: 73 143 1132 4545 Fragments arranged by size: 4545 1132 143 73 BamHI G'GATC_C Cuts at: 3223 3590 3223 Size: 367 5526 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 2359 3214 4669 618 Size: 325 217 627 240 332 855 1455 1842 Fragments arranged by size: 1842 1455 855 627 332 325 240 217 BanII G_rGCy'C Cuts at: 1391 2105 2183 2307 2608 2979 3212 1391 Size: 714 78 124 301 371 233 4072 Fragments arranged by size: 4072 714 371 301 233 124 78 BbeI G_GCGC'C Cuts at: 2031 2031 Size: 5893 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2441 2687 3132 928 Size: 971 542 246 445 3689 Fragments arranged by size: 3689 971 542 445 246 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4084 4118 1903 Size: 34 2147 34 3678 Fragments arranged by size: 3678 2147 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3785 4995 3785 Size: 1210 4683 BclI T'GATC_A Cuts at: 1548 1548 Size: 5893 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 4504 136 Size: 107 122 71 631 605 367 2465 1525 Fragments arranged by size: 2465 1525 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 5888 5888 Size: 5893 BlpI GC'TnA_GC Cuts at: 2051 3310 2051 Size: 1259 4634 Bme1580I G_kGCm'C Cuts at: 1790 2044 2444 3954 5110 1790 Size: 254 400 1510 1156 2573 Fragments arranged by size: 2573 1510 1156 400 254 BmgBI CAC'GTC Cuts at: 1318 2439 1318 Size: 1121 4772 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2608 2926 3624 Size: 416 53 83 399 24 1191 318 698 Cuts at: 3624 4582 442 Size: 958 1753 Fragments arranged by size: 1753 1191 958 698 416 399 318 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5893 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2586 2586 Size: 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 2321 3110 4553 1239 Size: 1055 27 789 1443 2579 Fragments arranged by size: 2579 1443 1055 789 27 Bpu10I CC'TnA_GC Cuts at: 2973 3168 2973 Size: 195 5698 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4018 4883 5181 4018 Size: 865 298 4730 Fragments arranged by size: 4730 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4556 882 Size: 617 3057 2219 Fragments arranged by size: 3057 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3190 3684 493 Size: 711 1986 494 2702 Fragments arranged by size: 2702 1986 711 494 BsaBI GATnn'nnATC Cuts at: 5887 5887 Size: 5893 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2028 3700 4082 4717 Size: 53 83 186 936 495 1672 382 635 Cuts at: 4717 275 Size: 1451 Fragments arranged by size: 1672 1451 936 635 495 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2584 4326 4724 4998 5145 5556 2584 Size: 1742 398 274 147 411 2921 Fragments arranged by size: 2921 1742 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3071 3101 1178 Size: 30 165 30 1668 30 3970 Fragments arranged by size: 3970 1668 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3079 1186 Size: 1893 4000 BseYI C'CCAG_C Cuts at: 2415 2608 5096 5592 2415 Size: 193 2488 496 2716 Fragments arranged by size: 2716 2488 496 193 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5320 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2048 2118 3343 4104 5132 1540 Size: 275 186 47 70 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 70 47 BsiHKAI G_wGCw'C Cuts at: 2979 3954 4039 5110 2979 Size: 975 85 1071 3762 Fragments arranged by size: 3762 1071 975 85 BsmI GAATG_Cn' Cuts at: 2730 3404 3497 2730 Size: 674 93 5126 Fragments arranged by size: 5126 674 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2105 2183 2307 2444 2608 2979 Size: 399 254 61 78 124 137 164 371 Cuts at: 2979 3212 3954 4039 5110 1391 Size: 233 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 371 254 233 164 137 124 85 78 61 BspEI T'CCGG_A Cuts at: 5556 5556 Size: 5893 BspHI T'CATG_A Cuts at: 3782 5512 3782 Size: 1730 4163 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2133 3248 844 Size: 1289 1115 3489 Fragments arranged by size: 3489 1289 1115 BsrBI CCG'CTC Cuts at: 1394 1823 2083 3118 3780 1394 Size: 429 260 1035 662 3507 Fragments arranged by size: 3507 1035 662 429 260 BsrDI GCAATG_nn' Cuts at: 65 1248 4388 4562 65 Size: 1183 3140 174 1396 Fragments arranged by size: 3140 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2117 4537 4724 1700 Size: 110 307 2420 187 2869 Fragments arranged by size: 2869 2420 307 187 110 BsrGI T'GTAC_A Cuts at: 96 3045 96 Size: 2949 2944 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5893 BssSI C'ACGA_G Cuts at: 3953 4965 3953 Size: 1012 4881 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5893 BstBI TT'CG_AA Cuts at: 2007 4697 2007 Size: 2690 3203 BstEII G'GTnAC_C Cuts at: 1184 2120 1184 Size: 936 4957 BstXI CCAn_nnnn'nTGG Cuts at: 3074 3074 Size: 5893 BstYI r'GATC_y Cuts at: 1972 2889 3223 3590 3975 3992 5444 5530 5542 Size: 917 334 367 385 17 1452 86 12 Cuts at: 5542 5888 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 917 385 367 346 334 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3024 3024 Size: 5893 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4384 Fragments arranged by size: 4384 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5154 Fragments arranged by size: 5154 683 56 BtsI GCAGTG_nn' Cuts at: 2825 3096 3409 4203 4223 2825 Size: 271 313 794 20 4495 Fragments arranged by size: 4495 794 313 271 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5893 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5858 DraI TTT'AAA Cuts at: 3197 3197 Size: 5893 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4900 809 Size: 507 3584 1802 Fragments arranged by size: 3584 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2045 2115 3055 Size: 54 1425 50 275 18 215 70 940 Cuts at: 3055 3340 4229 5858 8 Size: 285 889 1629 43 Fragments arranged by size: 1629 1425 940 889 285 275 215 70 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2045 2115 3340 1537 Size: 275 233 70 1225 4090 Fragments arranged by size: 4090 1225 275 233 70 EarI CTCTTCn'nnn_ Cuts at: 1353 2993 3823 1353 Size: 1640 830 3423 Fragments arranged by size: 3423 1640 830 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4480 4854 5000 387 Size: 1064 138 2891 374 146 1280 Fragments arranged by size: 2891 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2977 2977 Size: 5893 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2321 2515 2798 2903 3038 3110 Size: 393 662 27 194 283 105 135 72 Cuts at: 3110 3956 4553 5340 5608 1239 Size: 846 597 787 268 1524 Fragments arranged by size: 1524 846 787 662 597 393 283 268 194 135 105 72 27 EcoO109I rG'GnC_Cy Cuts at: 2110 3121 3162 2110 Size: 1011 41 4841 Fragments arranged by size: 4841 1011 41 EcoRI G'AATT_C Cuts at: 3202 3580 3202 Size: 378 5515 FspI TGC'GCA Cuts at: 2741 4399 2741 Size: 1658 4235 HaeII r_GCGC'y Cuts at: 1678 2031 2152 5040 1678 Size: 353 121 2888 2531 Fragments arranged by size: 2888 2531 353 121 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2918 2950 4534 4566 4608 Size: 32 135 32 1346 32 1584 32 42 Cuts at: 4608 4640 1373 Size: 32 2626 Fragments arranged by size: 2626 1584 1346 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3040 3237 3483 669 Size: 988 281 1102 197 246 3079 Fragments arranged by size: 3079 1102 988 281 246 197 HindIII A'AGCT_T Cuts at: 748 748 Size: 5893 HpaI GTT'AAC Cuts at: 3483 3483 Size: 5893 KasI G'GCGC_C Cuts at: 2027 2027 Size: 5893 KpnI G_GTAC'C Cuts at: 3218 3218 Size: 5893 MfeI C'AATT_G Cuts at: 3492 3492 Size: 5893 MluI A'CGCG_T Cuts at: 5769 5769 Size: 5893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2487 3004 5007 5191 1305 Size: 1182 517 2003 184 2007 Fragments arranged by size: 2007 2003 1182 517 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4071 Fragments arranged by size: 4071 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3851 4210 4369 518 Size: 648 2685 359 159 2042 Fragments arranged by size: 2685 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2846 3309 3986 5134 5379 1606 Size: 444 796 463 677 1148 245 2120 Fragments arranged by size: 2120 1148 796 677 463 444 245 NarI GG'CG_CC Cuts at: 2028 2028 Size: 5893 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4384 Fragments arranged by size: 4384 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5893 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5893 NotI GC'GGCC_GC Cuts at: 3340 3340 Size: 5893 NspI r_CATG'y Cuts at: 1132 1229 3251 4796 1132 Size: 97 2022 1545 2229 Fragments arranged by size: 2229 2022 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5893 PciI A'CATG_T Cuts at: 4792 4792 Size: 5893 PflMI CCAn_nnn'nTGG Cuts at: 1108 2370 1108 Size: 1262 4631 PmeI GTTT'AAAC Cuts at: 3197 3197 Size: 5893 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 5475 5507 3962 Size: 32 1481 32 4348 Fragments arranged by size: 4348 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2110 3121 2110 Size: 1011 4882 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5893 PsiI TTA'TAA Cuts at: 3463 3463 Size: 5893 PstI C_TGCA'G Cuts at: 830 2794 3245 830 Size: 1964 451 3478 Fragments arranged by size: 3478 1964 451 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5893 SacI G_AGCT'C Cuts at: 2979 2979 Size: 5893 SalI G'TCGA_C Cuts at: 1936 3038 3235 1936 Size: 1102 197 4594 Fragments arranged by size: 4594 1102 197 SanDI GG'GwC_CC Cuts at: 2110 2110 Size: 5893 SapI GCTCTTCn'nnn_ Cuts at: 2993 2993 Size: 5893 SbfI CC_TGCA'GG Cuts at: 3245 3245 Size: 5893 ScaI AGT'ACT Cuts at: 2291 4141 5853 2291 Size: 1850 1712 2331 Fragments arranged by size: 2331 1850 1712 SfcI C'TryA_G Cuts at: 826 1045 2542 2645 2790 2830 3241 4376 5057 Size: 219 1497 103 145 40 411 1135 681 Cuts at: 5057 5248 826 Size: 191 1471 Fragments arranged by size: 1497 1471 1135 681 411 219 191 145 103 40 SfoI GGC'GCC Cuts at: 2029 2029 Size: 5893 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5893 SmaI CCC'GGG Cuts at: 1872 2088 3220 1872 Size: 216 1132 4545 Fragments arranged by size: 4545 1132 216 SmlI C'TyrA_G Cuts at: 820 1017 1943 3997 4898 5160 820 Size: 197 926 2054 901 262 1553 Fragments arranged by size: 2054 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3190 3684 493 Size: 2697 494 2702 Fragments arranged by size: 2702 2697 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5893 SphI G_CATG'C Cuts at: 1132 3251 1132 Size: 2119 3774 SspI AAT'ATT Cuts at: 5 52 3817 5 Size: 47 3765 2081 Fragments arranged by size: 3765 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5893 StyI C'CwwG_G Cuts at: 513 1065 2022 2214 2228 3332 513 Size: 552 957 192 14 1104 3074 Fragments arranged by size: 3074 1104 957 552 192 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2377 2381 2902 2926 3920 3937 4090 Size: 1255 797 4 521 24 994 17 153 Cuts at: 4090 4275 4720 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 994 797 521 445 185 153 24 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2289 2652 3045 Size: 274 80 33 51 1443 312 363 393 Cuts at: 3045 4139 5851 96 Size: 1094 1712 138 Fragments arranged by size: 1712 1443 1094 393 363 312 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5853 5885 5853 Size: 32 5861 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5893 XbaI T'CTAG_A Cuts at: 3229 3229 Size: 5893 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5893 XmaI C'CCGG_G Cuts at: 1870 2086 3218 1870 Size: 216 1132 4545 Fragments arranged by size: 4545 1132 216 XmnI GAAnn'nnTTC Cuts at: 2291 4022 2291 Size: 1731 4162 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3701 276 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SrfI SwaI XcmI