TRAM2 ORF clone pFN21ASDA0057

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21ASDA0057

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Clone info.

Species

human

Clone Name

pFN21ASDA0057 Sequence (with vector) Restriction Map

Product ID

FHC00388

Flexi Clone PID

FXC00388

ORF Sequence

DNA sequence (1110 bp)
Protein sequence (370 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383729]

Gene

KIAA0057

Symbol

TRAM2

Description

translocation associated membrane protein 2

Original Clone ID

ha00909

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1110 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 370 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34477.1 3.6e-171 100.0 TRAM2 CCDS6207.1 5.2e-89 52.5 TRAM1 CCDS3707.1 7.5e-70 44.9 TRAM1L1 CCDS83300.1 1.4e-69 53.6 TRAM1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013599 48 111 PF08390 TRAM1-like protein IPR006634 113 311 PF03798 TRAM/LAG1/CLN8 homology domain HMMSmart IPR006634 112 321 SM00724 TRAM/LAG1/CLN8 homology domain ProfileScan IPR006634 112 321 PS50922 TRAM/LAG1/CLN8 homology domain
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 29 LVLCVLIGLMFEVTAKTAFLFIL 51 PRIMARY 23 2 73 KDLVTILFYIFITIILHAVVQEY 95 PRIMARY 23 3 118 GQLVVFHFTSVIWCFYVVVTEGY 140 SECONDARY 23 4 156 LPFQVKFFYLCQLAYWLHALPEL 178 SECONDARY 23 5 195 ICLYLVHIAGAYLLNLSRLGLIL 217 PRIMARY 23 6 248 WAAVFGVTRLFILTLAVLAIGFG 270 PRIMARY 23 7 286 FNTLFCRLCVLLLVCAAQAWLMW 308 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_036420 370 608485 100.0 100.0 XP_011513307 333 608485 100.0 83.5 NP_055109 374 605190 52.5 99.7 NP_001304733 343 605190 52.3 91.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0057 Download

>KIAA0057  1110 bp
ATGGCTTTCCGCAGGAGGACGAAAAGTTACCCGCTCTTCAGCCAGGAGTTCGTCATCCAC
AACCATGCGGACATCGGCTTCTGCCTGGTGCTCTGCGTCCTCATCGGGCTTATGTTCGAG
GTCACAGCCAAGACTGCCTTTCTATTTATTTTACCTCAGTATAACATTAGCGTGCCTACA
GCAGACAGTGAGACCGTGCACTACCACTATGGCCCTAAGGACCTGGTCACAATCTTGTTC
TACATCTTCATCACCATCATCTTGCATGCTGTGGTTCAGGAGTACATTTTAGATAAAATC
AGCAAACGGCTTCATCTCTCCAAAGTCAAACACAGCAAGTTCAATGAATCTGGACAGCTG
GTCGTCTTTCATTTCACCTCGGTGATTTGGTGCTTCTACGTGGTGGTGACGGAAGGATAC
TTAACAAACCCAAGAAGCCTCTGGGAAGACTACCCGCATGTGCACCTCCCCTTCCAGGTG
AAGTTTTTCTACCTATGCCAGCTGGCCTACTGGCTGCACGCACTTCCTGAGCTATACTTC
CAGAAGGTACGGAAGGAGGAAATTCCCCGCCAGCTCCAGTATATTTGCCTGTACCTGGTG
CATATAGCTGGAGCATACCTCTTAAACCTGAGCCGCCTGGGCCTGATCTTGCTGCTGCTG
CAGTACTCAACTGAGTTCCTCTTCCACACGGCTAGACTCTTCTACTTTGCAGATGAAAAC
AACGAGAAACTGTTCAGTGCCTGGGCTGCTGTTTTTGGGGTTACCCGCCTCTTCATCCTC
ACCCTTGCCGTGCTGGCCATTGGCTTTGGACTGGCTCGCATGGAAAACCAGGCATTTGAT
CCCGAGAAAGGGAACTTCAACACTTTGTTTTGCAGGCTCTGCGTGCTGCTGCTGGTGTGT
GCCGCCCAGGCCTGGCTCATGTGGCGCTTCATCCACTCCCAGCTGCGGCACTGGCGGGAA
TACTGGAATGAGCAGAGTGCAAAGCGGAGAGTCCCAGCCACACCCAGACTACCAGCCAGG
CTCATCAAGAGGGAATCTGGTTACCATGAAAATGGAGTGGTGAAGGCAGAGAACGGAACC
TCCCCACGGACTAAGAAACTCAAGTCTCCC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0057 Download

>KIAA0057  370 aa
MAFRRRTKSYPLFSQEFVIHNHADIGFCLVLCVLIGLMFEVTAKTAFLFILPQYNISVPT
ADSETVHYHYGPKDLVTILFYIFITIILHAVVQEYILDKISKRLHLSKVKHSKFNESGQL
VVFHFTSVIWCFYVVVTEGYLTNPRSLWEDYPHVHLPFQVKFFYLCQLAYWLHALPELYF
QKVRKEEIPRQLQYICLYLVHIAGAYLLNLSRLGLILLLLQYSTEFLFHTARLFYFADEN
NEKLFSAWAAVFGVTRLFILTLAVLAIGFGLARMENQAFDPEKGNFNTLFCRLCVLLLVC
AAQAWLMWRFIHSQLRHWREYWNEQSAKRRVPATPRLPARLIKRESGYHENGVVKAENGT
SPRTKKLKSP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0057 Download

>pFN21ASDA0057  5839 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCTTTCCGCAGGAG
GACGAAAAGTTACCCGCTCTTCAGCCAGGAGTTCGTCATCCACAACCATGCGGACATCGG
CTTCTGCCTGGTGCTCTGCGTCCTCATCGGGCTTATGTTCGAGGTCACAGCCAAGACTGC
CTTTCTATTTATTTTACCTCAGTATAACATTAGCGTGCCTACAGCAGACAGTGAGACCGT
GCACTACCACTATGGCCCTAAGGACCTGGTCACAATCTTGTTCTACATCTTCATCACCAT
CATCTTGCATGCTGTGGTTCAGGAGTACATTTTAGATAAAATCAGCAAACGGCTTCATCT
CTCCAAAGTCAAACACAGCAAGTTCAATGAATCTGGACAGCTGGTCGTCTTTCATTTCAC
CTCGGTGATTTGGTGCTTCTACGTGGTGGTGACGGAAGGATACTTAACAAACCCAAGAAG
CCTCTGGGAAGACTACCCGCATGTGCACCTCCCCTTCCAGGTGAAGTTTTTCTACCTATG
CCAGCTGGCCTACTGGCTGCACGCACTTCCTGAGCTATACTTCCAGAAGGTACGGAAGGA
GGAAATTCCCCGCCAGCTCCAGTATATTTGCCTGTACCTGGTGCATATAGCTGGAGCATA
CCTCTTAAACCTGAGCCGCCTGGGCCTGATCTTGCTGCTGCTGCAGTACTCAACTGAGTT
CCTCTTCCACACGGCTAGACTCTTCTACTTTGCAGATGAAAACAACGAGAAACTGTTCAG
TGCCTGGGCTGCTGTTTTTGGGGTTACCCGCCTCTTCATCCTCACCCTTGCCGTGCTGGC
CATTGGCTTTGGACTGGCTCGCATGGAAAACCAGGCATTTGATCCCGAGAAAGGGAACTT
CAACACTTTGTTTTGCAGGCTCTGCGTGCTGCTGCTGGTGTGTGCCGCCCAGGCCTGGCT
CATGTGGCGCTTCATCCACTCCCAGCTGCGGCACTGGCGGGAATACTGGAATGAGCAGAG
TGCAAAGCGGAGAGTCCCAGCCACACCCAGACTACCAGCCAGGCTCATCAAGAGGGAATC
TGGTTACCATGAAAATGGAGTGGTGAAGGCAGAGAACGGAACCTCCCCACGGACTAAGAA
ACTCAAGTCTCCCTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT