DENND6A ORF clone pFN21AF0229

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Halotag ORF Clone pFN21AF0229

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Clone info.

Species

human

Clone Name

pFN21AF0229 Sequence (with vector) Restriction Map

Product ID

FHC01654

Flexi Clone PID

FXC01654

ORF Sequence

DNA sequence (1824 bp)
Protein sequence (608 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385262]

Gene

FLJ00229

Symbol

DENND6A

Alias : AFI1A, FAM116A

Description

DENN/MADD domain containing 6A

Original Clone ID

sj06546

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1824 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 608 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33773.1 0 99.8 DENND6A CCDS46732.1 1.5e-149 60.6 DENND6B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR012860 46 603 PF07792 Protein of unknown function DUF1630 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AF0229 Download

>FLJ00229  1824 bp
ATGGCTTTGAGGGGCCCTGCGGGCTTGGGGCCCGGCTCTCGAAGGCCGTTGGACGAAGCG
GTGGCAGGGGCCGAGGGCCGCGAGGCGCCGGCCCTTGTGGCGGCGGGAGGCGCGCCAGAG
GACGATGAAGAGGACGATGGCCGTGGCCGGGGCCTGCTGCGCTGGGACAGCTTCTCCGCC
TGGCTGCACTGCGTGTGTGTGGTGGGCTTCGACCTGGAGCTGGGCCAGGCCGTGGAGGTA
ATTTATCCTCAGCATTCCAAACTTACTGACAGAGAAAAAACCAATATTTGCTATTTGTCT
TTTCCAGATTCAAATTCAGGTTGTCTTGGAGATACCCAGTTTTGTTTTAGATTTCGACAG
TCTTCTGGGAGGAGGGTGTCGCTGCATTGTCTCCTGGATCAATTTGACAAAGATTTACCA
GTTTACTTAAAGAAGGATCCTGCTTATTTTTATGGATATGTGTATTTCCGACAAGTTCGA
GATAAAACTCTAAAAAGAGGCTACTTTCAGAAGTCCTTGGTTTTGATCAGCAAACTACCT
TATATTCATTTTTTTCACACTGTGCTCAAACAGATAGCACCAGAGTATTTTGAAAAGAAT
GAACCTTATTTGGAAGCAGCTTGTAATGATGTTGATCGATGGCCTGCCCCAGTGCCAGGG
AAAACATTACACCTGCCAATCATGGCGGTGGTAATGAAGGTACGGATTCCCACATGTCAT
GACAAGCCTGGGACAACTCAAATAGTGCAGTTAACTCAGCAGGTGGACACAAATATATCT
GTTATTTTACCTACTGTTCATGAGGTGGATATTTTCAGGTGTTTCTGCCCAGTTTTCCTT
CATAGTCAGATGCTCTGGGAGCTGGTGCTGTTGGGGGAGCCCCTTGTGGTTATGGCGCCA
TCACCATCGGAATCATCAGAGACTGTATTGGCACTTGTTAACTGTATTTCTCCATTAAAG
TACTTCAGTGATTTCCGACCTTATTTCACTATTCATGATAGTGAATTCAAAGAATATACT
ACCCGTACCCAAGCTCCGCCCTCAGTTATATTAGGAGTAACCAACCCTTTTTTTGCTAAG
ACACTCCAGCACTGGCCACACATTATTCGAATAGGAGACCTTAAACCTACAGGTGAAATT
CCTAAGCAGGTTAAAGTGAAAAAACTGAAGAATCTAAAGACTCTGGATTCCAAACCTGGA
GTTTATACTTCATATAAGCCATATTTAAATAGAGATGAAGAGATCATAAAACAATTACAG
AAGGGTGTACAACAGAAACGTCCTTCTGAGGCTCAAAGTGTTATTCTTCGACGCTATTTT
TTGGAACTGACACAAAGTTTCATCATTCCATTAGAAAGATATGTGGCAAGCTTGATGCCT
TTGCAGAAAAGTATTTCCCCATGGAAGAGTCCACCTCAATTAAGACAGTTTCTTCCAGAA
GAATTTATGAAAACACTTGAGAAAACAGGACCTCAGCTAACCTCTAGAATAAAAGGCGAT
TGGATTGGACTTTACCGGCATTTCCTAAAGTCTCCAAATTTTGATGGCTGGTTTAAGACC
CGGAGGAAGGAAATGACCCAAAAATTGGAGGCACTCCATCTAGAAGCTCTTTGTGAAGAG
GACTTACTTCTCTGGATCCAGAAACACACAGAAGTAGAAACAGTAGACCTTGTCTTGAAG
CTGAAAAATAAGCTGTTGCAGGCTGATCGAGAGCACTTACCTGTGAAACCTGACACTATG
GAAAAGTTACGGACACACATAGATGCCATTATCTTAGCATTGCCAGAGGACTTGCAAGGC
ATACTGCTCAAAACGGGCATGACA

ORF Protein

Cloned ORF protein sequence for pFN21AF0229 Download

>FLJ00229  608 aa
MALRGPAGLGPGSRRPLDEAVAGAEGREAPALVAAGGAPEDDEEDDGRGRGLLRWDSFSA
WLHCVCVVGFDLELGQAVEVIYPQHSKLTDREKTNICYLSFPDSNSGCLGDTQFCFRFRQ
SSGRRVSLHCLLDQFDKDLPVYLKKDPAYFYGYVYFRQVRDKTLKRGYFQKSLVLISKLP
YIHFFHTVLKQIAPEYFEKNEPYLEAACNDVDRWPAPVPGKTLHLPIMAVVMKVRIPTCH
DKPGTTQIVQLTQQVDTNISVILPTVHEVDIFRCFCPVFLHSQMLWELVLLGEPLVVMAP
SPSESSETVLALVNCISPLKYFSDFRPYFTIHDSEFKEYTTRTQAPPSVILGVTNPFFAK
TLQHWPHIIRIGDLKPTGEIPKQVKVKKLKNLKTLDSKPGVYTSYKPYLNRDEEIIKQLQ
KGVQQKRPSEAQSVILRRYFLELTQSFIIPLERYVASLMPLQKSISPWKSPPQLRQFLPE
EFMKTLEKTGPQLTSRIKGDWIGLYRHFLKSPNFDGWFKTRRKEMTQKLEALHLEALCEE
DLLLWIQKHTEVETVDLVLKLKNKLLQADREHLPVKPDTMEKLRTHIDAIILALPEDLQG
ILLKTGMT

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AF0229 Download

>pFN21AF0229  6529 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTTTGAGGGGCCCTGCGGGCTTGGGGCCCGG
CTCTCGAAGGCCGTTGGACGAAGCGGTGGCAGGGGCCGAGGGCCGCGAGGCGCCGGCCCT
TGTGGCGGCGGGAGGCGCGCCAGAGGACGATGAAGAGGACGATGGCCGTGGCCGGGGCCT
GCTGCGCTGGGACAGCTTCTCCGCCTGGCTGCACTGCGTGTGTGTGGTGGGCTTCGACCT
GGAGCTGGGCCAGGCCGTGGAGGTAATTTATCCTCAGCATTCCAAACTTACTGACAGAGA
AAAAACCAATATTTGCTATTTGTCTTTTCCAGATTCAAATTCAGGTTGTCTTGGAGATAC
CCAGTTTTGTTTTAGATTTCGACAGTCTTCTGGGAGGAGGGTGTCGCTGCATTGTCTCCT
GGATCAATTTGACAAAGATTTACCAGTTTACTTAAAGAAGGATCCTGCTTATTTTTATGG
ATATGTGTATTTCCGACAAGTTCGAGATAAAACTCTAAAAAGAGGCTACTTTCAGAAGTC
CTTGGTTTTGATCAGCAAACTACCTTATATTCATTTTTTTCACACTGTGCTCAAACAGAT
AGCACCAGAGTATTTTGAAAAGAATGAACCTTATTTGGAAGCAGCTTGTAATGATGTTGA
TCGATGGCCTGCCCCAGTGCCAGGGAAAACATTACACCTGCCAATCATGGCGGTGGTAAT
GAAGGTACGGATTCCCACATGTCATGACAAGCCTGGGACAACTCAAATAGTGCAGTTAAC
TCAGCAGGTGGACACAAATATATCTGTTATTTTACCTACTGTTCATGAGGTGGATATTTT
CAGGTGTTTCTGCCCAGTTTTCCTTCATAGTCAGATGCTCTGGGAGCTGGTGCTGTTGGG
GGAGCCCCTTGTGGTTATGGCGCCATCACCATCGGAATCATCAGAGACTGTATTGGCACT
TGTTAACTGTATTTCTCCATTAAAGTACTTCAGTGATTTCCGACCTTATTTCACTATTCA
TGATAGTGAATTCAAAGAATATACTACCCGTACCCAAGCTCCGCCCTCAGTTATATTAGG
AGTAACCAACCCTTTTTTTGCTAAGACACTCCAGCACTGGCCACACATTATTCGAATAGG
AGACCTTAAACCTACAGGTGAAATTCCTAAGCAGGTTAAAGTGAAAAAACTGAAGAATCT
AAAGACTCTGGATTCCAAACCTGGAGTTTATACTTCATATAAGCCATATTTAAATAGAGA
TGAAGAGATCATAAAACAATTACAGAAGGGTGTACAACAGAAACGTCCTTCTGAGGCTCA
AAGTGTTATTCTTCGACGCTATTTTTTGGAACTGACACAAAGTTTCATCATTCCATTAGA
AAGATATGTGGCAAGCTTGATGCCTTTGCAGAAAAGTATTTCCCCATGGAAGAGTCCACC
TCAATTAAGACAGTTTCTTCCAGAAGAATTTATGAAAACACTTGAGAAAACAGGACCTCA
GCTAACCTCTAGAATAAAAGGCGATTGGATTGGACTTTACCGGCATTTCCTAAAGTCTCC
AAATTTTGATGGCTGGTTTAAGACCCGGAGGAAGGAAATGACCCAAAAATTGGAGGCACT
CCATCTAGAAGCTCTTTGTGAAGAGGACTTACTTCTCTGGATCCAGAAACACACAGAAGT
AGAAACAGTAGACCTTGTCTTGAAGCTGAAAAATAAGCTGTTGCAGGCTGATCGAGAGCA
CTTACCTGTGAAACCTGACACTATGGAAAAGTTACGGACACACATAGATGCCATTATCTT
AGCATTGCCAGAGGACTTGCAAGGCATACTGCTCAAAACGGGCATGACAGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT