(Circular) (Six-base) MAPSORT of: pFN21AF0229.seq Check: 7902 from: 1 to: 6529 pFN21AF0229 6529 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2685 2755 2685 Size: 70 6459 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4339 278 Size: 53 83 186 936 2803 2468 Fragments arranged by size: 2803 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3669 3872 1937 Size: 1732 203 4594 Fragments arranged by size: 4594 1732 203 Acc65I G'GTAC_C Cuts at: 3850 3850 Size: 6529 AclI AA'CG_TT Cuts at: 1563 4656 5029 1563 Size: 3093 373 3063 Fragments arranged by size: 3093 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2953 3191 4592 5976 6244 1632 Size: 1321 238 1401 1384 268 1917 Fragments arranged by size: 1917 1401 1384 1321 268 238 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6332 AflIII A'CryG_T Cuts at: 1203 2717 5428 6405 1203 Size: 1514 2711 977 1327 Fragments arranged by size: 2711 1514 1327 977 AgeI A'CCGG_T Cuts at: 5360 5360 Size: 6529 AhdI GACnn_n'nnGTC Cuts at: 5258 5258 Size: 6529 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6529 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6529 AlwNI CAG_nnn'CTG Cuts at: 2927 4272 5844 2927 Size: 1345 1572 3612 Fragments arranged by size: 3612 1572 1345 ApaI G_GGCC'C Cuts at: 2021 2037 2021 Size: 16 6513 ApaLI G'TGCA_C Cuts at: 4586 5742 4586 Size: 1156 5373 ApoI r'AATT_y Cuts at: 1493 2317 3008 3141 3446 3541 3838 4064 4216 Size: 824 691 133 305 95 297 226 152 Cuts at: 4216 6325 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 824 691 305 297 226 152 133 95 AscI GG'CGCG_CC Cuts at: 2115 2115 Size: 6529 AseI AT'TA_AT Cuts at: 160 1032 5083 160 Size: 872 4051 1606 Fragments arranged by size: 4051 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6529 AvaI C'yCGr_G Cuts at: 1870 1943 3854 1870 Size: 73 1911 4545 Fragments arranged by size: 4545 1911 73 BamHI G'GATC_C Cuts at: 2440 3639 3859 4226 2440 Size: 1199 220 367 4743 Fragments arranged by size: 4743 1199 367 220 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2089 2899 3850 5305 618 Size: 325 217 627 302 810 951 1455 1842 Fragments arranged by size: 1842 1455 951 810 627 325 302 217 BanII G_rGCy'C Cuts at: 1391 2021 2037 2886 3848 1391 Size: 630 16 849 962 4072 Fragments arranged by size: 4072 962 849 630 16 BbeI G_GCGC'C Cuts at: 2093 2903 2093 Size: 810 5719 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2358 928 Size: 971 459 5099 Fragments arranged by size: 5099 971 459 BbvCI CC'TCA_GC Cuts at: 2253 3477 2253 Size: 1224 5305 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3692 3726 4720 4754 1903 Size: 34 1755 34 994 34 3678 Fragments arranged by size: 3678 1755 994 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4421 5631 4421 Size: 1210 5319 BclI T'GATC_A Cuts at: 1548 2529 1548 Size: 981 5548 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2025 2102 5140 Size: 107 122 71 631 605 353 77 3038 Cuts at: 5140 136 Size: 1525 Fragments arranged by size: 3038 1525 631 605 353 122 107 77 71 BglII A'GATC_T Cuts at: 6524 6524 Size: 6529 BlpI GC'TnA_GC Cuts at: 3946 3946 Size: 6529 Bme1580I G_kGCm'C Cuts at: 1790 2021 2037 4590 5746 1790 Size: 231 16 2553 1156 2573 Fragments arranged by size: 2573 2553 1156 231 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6529 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2335 2648 2828 Size: 416 53 83 399 24 918 313 180 Cuts at: 2828 4260 5218 442 Size: 1432 958 1753 Fragments arranged by size: 1753 1432 958 918 416 399 313 180 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6529 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3435 3435 Size: 6529 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 3074 3222 5189 1239 Size: 1001 834 148 1967 2579 Fragments arranged by size: 2579 1967 1001 834 148 Bpu10I CC'TnA_GC Cuts at: 2253 3147 3477 2253 Size: 894 330 5305 Fragments arranged by size: 5305 894 330 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3482 4654 5519 5817 3482 Size: 1172 865 298 4194 Fragments arranged by size: 4194 1172 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3114 5192 882 Size: 617 1615 2078 2219 Fragments arranged by size: 2219 2078 1615 617 BsaAI yAC'GTr Cuts at: 493 1204 4320 493 Size: 711 3116 2702 Fragments arranged by size: 3116 2702 711 BsaBI GATnn'nnATC Cuts at: 2637 6523 2637 Size: 3886 2643 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2090 2900 4336 4718 Size: 53 83 186 936 557 810 1436 382 Cuts at: 4718 5353 275 Size: 635 1451 Fragments arranged by size: 1451 1436 936 810 635 557 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4962 5360 5634 5781 6192 4962 Size: 398 274 147 411 5299 Fragments arranged by size: 5299 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2162 2192 3022 3052 1178 Size: 30 165 30 759 30 830 30 4655 Fragments arranged by size: 4655 830 759 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2389 1186 Size: 1203 5326 BseYI C'CCAG_C Cuts at: 2166 2224 5732 6228 2166 Size: 58 3508 496 2467 Fragments arranged by size: 3508 2467 496 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2174 2771 1163 Size: 573 438 597 4921 Fragments arranged by size: 4921 597 573 438 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3979 4740 5768 1540 Size: 275 186 1978 761 1028 2301 Fragments arranged by size: 2301 1978 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2571 3720 4590 4675 5746 2571 Size: 1149 870 85 1071 3354 Fragments arranged by size: 3354 1149 1071 870 85 BsmI GAATG_Cn' Cuts at: 2257 4040 4133 2257 Size: 1783 93 4653 Fragments arranged by size: 4653 1783 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2037 2571 2886 3720 3848 4590 Size: 399 231 16 534 315 834 128 742 Cuts at: 4590 4675 5746 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 834 742 534 399 315 231 128 85 16 BspEI T'CCGG_A Cuts at: 6192 6192 Size: 6529 BspHI T'CATG_A Cuts at: 2722 2803 2998 4418 6148 2722 Size: 81 195 1420 1730 3103 Fragments arranged by size: 3103 1730 1420 195 81 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2685 2755 3142 3884 844 Size: 1841 70 387 742 3489 Fragments arranged by size: 3489 1841 742 387 70 BsrBI CCG'CTC Cuts at: 1394 1823 4416 1394 Size: 429 2593 3507 Fragments arranged by size: 3507 2593 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3782 5024 5198 65 Size: 1183 2534 1242 174 1396 Fragments arranged by size: 2534 1396 1242 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2092 3519 5173 5360 1700 Size: 110 282 1427 1654 187 2869 Fragments arranged by size: 2869 1654 1427 282 187 110 BsrGI T'GTAC_A Cuts at: 96 3271 96 Size: 3175 3354 BssHII G'CGCG_C Cuts at: 1925 2115 1925 Size: 190 6339 BssSI C'ACGA_G Cuts at: 4589 5601 4589 Size: 1012 5517 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6529 BstBI TT'CG_AA Cuts at: 3112 5333 3112 Size: 2221 4308 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6529 BstYI r'GATC_y Cuts at: 1972 2440 3639 3859 4226 4611 4628 6080 6166 Size: 468 1199 220 367 385 17 1452 86 Cuts at: 6166 6178 6524 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1199 468 385 367 346 220 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2146 2235 3404 513 Size: 552 939 142 89 1169 3638 Fragments arranged by size: 3638 1169 939 552 142 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5790 Fragments arranged by size: 5790 683 56 BtsI GCAGTG_nn' Cuts at: 2191 4045 4839 4859 2191 Size: 1854 794 20 3861 Fragments arranged by size: 3861 1854 794 20 ClaI AT'CG_AT Cuts at: 1554 2641 1554 Size: 1087 5442 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6494 DraI TTT'AAA Cuts at: 3231 3833 3231 Size: 602 5927 DraIII CAC_nnn'GTG Cuts at: 2197 2566 2197 Size: 369 6160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5536 809 Size: 507 4220 1802 Fragments arranged by size: 4220 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2143 2149 3098 Size: 54 1425 50 275 18 313 6 949 Cuts at: 3098 3976 4865 6494 8 Size: 878 889 1629 43 Fragments arranged by size: 1629 1425 949 889 878 313 275 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3976 1537 Size: 275 2164 4090 Fragments arranged by size: 4090 2164 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 3237 3404 3615 4459 1353 Size: 774 1110 167 211 844 3423 Fragments arranged by size: 3423 1110 844 774 211 167 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2170 3030 5116 5490 5636 387 Size: 1064 138 581 860 2086 374 146 1280 Fragments arranged by size: 2086 1280 1064 860 581 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2953 3074 3191 3222 4592 5189 Size: 393 608 713 121 117 31 1370 597 Cuts at: 5189 5976 6244 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1370 787 713 608 597 393 268 121 117 31 EcoO109I rG'GnC_Cy Cuts at: 2017 2018 2033 2155 3473 2017 Size: 1 15 122 1318 5073 Fragments arranged by size: 5073 1318 122 15 1 EcoRI G'AATT_C Cuts at: 3008 3838 4216 3008 Size: 830 378 5321 Fragments arranged by size: 5321 830 378 FspI TGC'GCA Cuts at: 5035 5035 Size: 6529 HaeII r_GCGC'y Cuts at: 1678 2093 2903 5676 1678 Size: 415 810 2773 2531 Fragments arranged by size: 2773 2531 810 415 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2162 2194 5170 5202 5244 Size: 32 135 32 590 32 2976 32 42 Cuts at: 5244 5276 1373 Size: 32 2626 Fragments arranged by size: 2976 2626 590 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2757 2944 3873 4119 669 Size: 988 281 819 187 929 246 3079 Fragments arranged by size: 3079 988 929 819 281 246 187 HindIII A'AGCT_T Cuts at: 748 3373 748 Size: 2625 3904 HpaI GTT'AAC Cuts at: 2757 2944 4119 2757 Size: 187 1175 5167 Fragments arranged by size: 5167 1175 187 KasI G'GCGC_C Cuts at: 2089 2899 2089 Size: 810 5719 KpnI G_GTAC'C Cuts at: 3854 3854 Size: 6529 MfeI C'AATT_G Cuts at: 4128 4128 Size: 6529 MluI A'CGCG_T Cuts at: 6405 6405 Size: 6529 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2034 2497 3004 5643 5827 1305 Size: 729 463 507 2639 184 2007 Fragments arranged by size: 2639 2007 729 507 463 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3100 10 Size: 54 1425 343 1268 3439 Fragments arranged by size: 3439 1425 1268 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2690 3003 3765 4487 4846 5005 518 Size: 648 1524 313 762 722 359 159 2042 Fragments arranged by size: 2042 1524 762 722 648 359 313 159 MspA1I CmG'CkG Cuts at: 1606 3945 4622 5770 6015 1606 Size: 2339 677 1148 245 2120 Fragments arranged by size: 2339 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2094 2094 Size: 6529 NarI GG'CG_CC Cuts at: 2090 2900 2090 Size: 810 5719 NcoI C'CATG_G Cuts at: 513 1065 2004 3404 513 Size: 552 939 1400 3638 Fragments arranged by size: 3638 1400 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6529 NgoMIV G'CCGG_C Cuts at: 2092 2092 Size: 6529 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6529 NotI GC'GGCC_GC Cuts at: 3976 3976 Size: 6529 NspI r_CATG'y Cuts at: 1132 1229 2721 3887 5432 1132 Size: 97 1492 1166 1545 2229 Fragments arranged by size: 2229 1545 1492 1166 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6529 PciI A'CATG_T Cuts at: 2717 5428 2717 Size: 2711 3818 PflMI CCAn_nnn'nTGG Cuts at: 1108 2236 3589 1108 Size: 1128 1353 4048 Fragments arranged by size: 4048 1353 1128 PfoI T'CCnGG_A Cuts at: 2397 2397 Size: 6529 PmeI GTTT'AAAC Cuts at: 3833 3833 Size: 6529 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4598 4630 6111 6143 4598 Size: 32 1481 32 4984 Fragments arranged by size: 4984 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3473 3473 Size: 6529 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6529 PsiI TTA'TAA Cuts at: 4099 4099 Size: 6529 PspOMI G'GGCC_C Cuts at: 2017 2033 2017 Size: 16 6513 PstI C_TGCA'G Cuts at: 830 3881 830 Size: 3051 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6529 SalI G'TCGA_C Cuts at: 1936 3871 1936 Size: 1935 4594 SbfI CC_TGCA'GG Cuts at: 3881 3881 Size: 6529 ScaI AGT'ACT Cuts at: 2966 4777 6489 2966 Size: 1811 1712 3006 Fragments arranged by size: 3006 1811 1712 SfcI C'TryA_G Cuts at: 826 1045 3132 3877 5012 5693 5884 826 Size: 219 2087 745 1135 681 191 1471 Fragments arranged by size: 2087 1471 1135 745 681 219 191 SfoI GGC'GCC Cuts at: 2091 2901 2091 Size: 810 5719 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6529 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6529 SmaI CCC'GGG Cuts at: 1872 3856 1872 Size: 1984 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3461 4633 5534 5796 820 Size: 197 926 1518 1172 901 262 1553 Fragments arranged by size: 1553 1518 1172 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4320 493 Size: 3827 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6529 SphI G_CATG'C Cuts at: 1132 3887 1132 Size: 2755 3774 SspI AAT'ATT Cuts at: 5 52 2290 4453 5 Size: 47 2238 2163 2081 Fragments arranged by size: 2238 2163 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6529 StyI C'CwwG_G Cuts at: 513 1065 2004 2520 3404 3968 513 Size: 552 939 516 884 564 3074 Fragments arranged by size: 3074 939 884 564 552 516 SwaI ATTT'AAAT Cuts at: 3231 3231 Size: 6529 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4556 4573 4726 4911 5356 325 Size: 1255 2976 17 153 185 445 1498 Fragments arranged by size: 2976 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2964 3271 4775 Size: 274 80 33 51 1443 987 307 1504 Cuts at: 4775 6487 96 Size: 1712 138 Fragments arranged by size: 1712 1504 1443 987 307 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6489 6521 6489 Size: 32 6497 Tth111I GACn'n_nGTC Cuts at: 1316 3674 1316 Size: 2358 4171 XbaI T'CTAG_A Cuts at: 3488 3604 3865 3488 Size: 116 261 6152 Fragments arranged by size: 6152 261 116 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6529 XmaI C'CCGG_G Cuts at: 1870 3854 1870 Size: 1984 4545 XmnI GAAnn'nnTTC Cuts at: 4658 4658 Size: 6529 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4337 276 Size: 53 83 186 936 2803 2468 Fragments arranged by size: 2803 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI XcmI