OPN1SW ORF clone pFN21AE9524

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE9524

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Clone info.

Species

human

Clone Name

pFN21AE9524 Sequence (with vector) Restriction Map

Product ID

FHC23078

ORF Sequence

DNA sequence (1044 bp)
Protein sequence (348 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9524

Symbol

OPN1SW

Alias : BCP, BOP, CBT

Description

opsin 1 (cone pigments), short-wave-sensitive

Original Clone ID

cp99524

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1044 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 348 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5806.1 2.7e-102 100.0 OPN1SW CCDS3063.1 9.8e-44 45.9 RHO CCDS83509.1 2.2e-41 44.3 OPN1MW3 CCDS35447.1 2.2e-41 44.3 OPN1MW2 CCDS14743.1 2.2e-41 44.3 OPN1MW The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001521 4 12 PR00574 Opsin IPR001521 13 25 PR00574 Opsin IPR000276 36 60 PR00237 GPCR IPR001760 57 69 PR00238 Opsin IPR000276 69 90 PR00237 GPCR IPR000276 114 136 PR00237 GPCR IPR000276 149 170 PR00237 GPCR IPR001760 171 183 PR00238 Opsin IPR001521 190 200 PR00574 Opsin IPR001521 200 211 PR00574 Opsin IPR000276 201 224 PR00237 GPCR IPR000276 247 271 PR00237 GPCR IPR001521 267 286 PR00574 Opsin IPR001760 282 294 PR00238 Opsin IPR000276 285 311 PR00237 GPCR IPR001521 314 328 PR00574 Opsin HMMPfam IPR000276 52 303 PF00001 GPCR ProfileScan IPR017452 51 303 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 33 AFYLQAAFMGTVFLIGFPLNAMV 55 PRIMARY 23 2 72 ILVNVSFGGFLLCIFSVFPVFVA 94 PRIMARY 23 3 112 FLGTVAGLVTGWSLAFLAFERYI 134 SECONDARY 23 4 150 ALTVVLATWTIGIGVSIPPFFGW 172 SECONDARY 23 5 205 FIFCFIVPLSLICFSYTQLLRAL 227 PRIMARY 23 6 249 RMVVVMVGSFCVCYVPYAAFAMY 271 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001699 348 190900,613522 100.0 100.0 NP_000530 348 136880,180380,610445,613731 45.9 95.7 NP_000504 364 300821,303700,303800 44.3 98.6 NP_064445 364 300822,303700,303900 43.2 98.6 NP_055137 402 606695 30.5 86.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9524 Download

>KIEE9524  1044 bp
ATGAGAAAAATGTCGGAGGAAGAGTTTTATCTGTTCAAAAATATCTCTTCAGTGGGGCCG
TGGGATGGGCCTCAGTACCACATTGCCCCTGTCTGGGCCTTCTACCTCCAGGCAGCTTTC
ATGGGCACTGTCTTCCTTATAGGGTTCCCACTCAATGCCATGGTGCTGGTGGCCACACTG
CGCTACAAAAAGTTGCGGCAGCCCCTCAACTACATTCTGGTCAACGTGTCCTTCGGAGGC
TTCCTCCTCTGCATCTTCTCTGTCTTCCCTGTCTTCGTCGCCAGCTGTAACGGATACTTC
GTCTTCGGTCGCCATGTTTGTGCTTTGGAGGGCTTCCTGGGCACTGTAGCAGGTCTGGTT
ACAGGATGGTCACTGGCCTTCCTGGCCTTTGAGCGCTACATTGTCATCTGTAAGCCCTTC
GGCAACTTCCGCTTCAGCTCCAAGCATGCACTGACGGTGGTCCTGGCTACCTGGACCATT
GGTATTGGCGTCTCCATCCCACCCTTCTTTGGCTGGAGCCGGTTCATCCCTGAGGGCCTG
CAGTGTTCCTGTGGCCCTGACTGGTACACCGTGGGCACCAAATACCGCAGCGAGTCCTAT
ACGTGGTTCCTCTTCATCTTCTGCTTCATTGTGCCTCTCTCCCTCATCTGCTTCTCCTAC
ACTCAGCTGCTGAGGGCCCTGAAAGCTGTTGCAGCTCAGCAGCAGGAGTCAGCTACGACC
CAGAAGGCTGAACGGGAGGTGAGCCGCATGGTGGTTGTGATGGTAGGATCCTTCTGTGTC
TGCTACGTGCCCTACGCGGCCTTCGCCATGTACATGGTCAACAACCGTAACCATGGGCTG
GACTTACGGCTTGTCACCATTCCTTCATTCTTCTCCAAGAGTGCTTGCATCTACAATCCC
ATCATCTACTGCTTCATGAATAAGCAGTTCCAAGCTTGCATCATGAAGATGGTGTGTGGG
AAGGCCATGACAGATGAATCCGACACATGCAGCTCCCAGAAAACAGAAGTTTCTACTGTC
TCGTCTACCCAAGTTGGCCCCAAC

ORF Protein

Cloned ORF protein sequence for pFN21AE9524 Download

>KIEE9524  348 aa
MRKMSEEEFYLFKNISSVGPWDGPQYHIAPVWAFYLQAAFMGTVFLIGFPLNAMVLVATL
RYKKLRQPLNYILVNVSFGGFLLCIFSVFPVFVASCNGYFVFGRHVCALEGFLGTVAGLV
TGWSLAFLAFERYIVICKPFGNFRFSSKHALTVVLATWTIGIGVSIPPFFGWSRFIPEGL
QCSCGPDWYTVGTKYRSESYTWFLFIFCFIVPLSLICFSYTQLLRALKAVAAQQQESATT
QKAEREVSRMVVVMVGSFCVCYVPYAAFAMYMVNNRNHGLDLRLVTIPSFFSKSACIYNP
IIYCFMNKQFQACIMKMVCGKAMTDESDTCSSQKTEVSTVSSTQVGPN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9524 Download

>pFN21AE9524  5749 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAGAAAAATGTCGGAGGAAGAGTTTTATCTGTT
CAAAAATATCTCTTCAGTGGGGCCGTGGGATGGGCCTCAGTACCACATTGCCCCTGTCTG
GGCCTTCTACCTCCAGGCAGCTTTCATGGGCACTGTCTTCCTTATAGGGTTCCCACTCAA
TGCCATGGTGCTGGTGGCCACACTGCGCTACAAAAAGTTGCGGCAGCCCCTCAACTACAT
TCTGGTCAACGTGTCCTTCGGAGGCTTCCTCCTCTGCATCTTCTCTGTCTTCCCTGTCTT
CGTCGCCAGCTGTAACGGATACTTCGTCTTCGGTCGCCATGTTTGTGCTTTGGAGGGCTT
CCTGGGCACTGTAGCAGGTCTGGTTACAGGATGGTCACTGGCCTTCCTGGCCTTTGAGCG
CTACATTGTCATCTGTAAGCCCTTCGGCAACTTCCGCTTCAGCTCCAAGCATGCACTGAC
GGTGGTCCTGGCTACCTGGACCATTGGTATTGGCGTCTCCATCCCACCCTTCTTTGGCTG
GAGCCGGTTCATCCCTGAGGGCCTGCAGTGTTCCTGTGGCCCTGACTGGTACACCGTGGG
CACCAAATACCGCAGCGAGTCCTATACGTGGTTCCTCTTCATCTTCTGCTTCATTGTGCC
TCTCTCCCTCATCTGCTTCTCCTACACTCAGCTGCTGAGGGCCCTGAAAGCTGTTGCAGC
TCAGCAGCAGGAGTCAGCTACGACCCAGAAGGCTGAACGGGAGGTGAGCCGCATGGTGGT
TGTGATGGTAGGATCCTTCTGTGTCTGCTACGTGCCCTACGCGGCCTTCGCCATGTACAT
GGTCAACAACCGTAACCATGGGCTGGACTTACGGCTTGTCACCATTCCTTCATTCTTCTC
CAAGAGTGCTTGCATCTACAATCCCATCATCTACTGCTTCATGAATAAGCAGTTCCAAGC
TTGCATCATGAAGATGGTGTGTGGGAAGGCCATGACAGATGAATCCGACACATGCAGCTC
CCAGAAAACAGAAGTTTCTACTGTCTCGTCTACCCAAGTTGGCCCCAACGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT