PCYT2 ORF clone pFN21AE5546

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5546

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Clone info.

Species

human

Clone Name

pFN21AE5546 Sequence (with vector) Restriction Map

Product ID

FHC26222

ORF Sequence

DNA sequence (1167 bp)
Protein sequence (389 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5546

Symbol

PCYT2

Alias : ET, SPG82

Description

phosphate cytidylyltransferase 2, ethanolamine, transcript variant 2

Original Clone ID

oc95546

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1167 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 389 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11791.1 2.7e-180 100.0 PCYT2 CCDS62364.1 5.9e-163 100.0 PCYT2 CCDS58610.1 8.9e-141 100.0 PCYT2 CCDS82220.1 2.6e-139 94.3 PCYT2 CCDS54178.1 2.6e-93 95.6 PCYT2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004820 26 150 PF01467 Cytidylyltransferase IPR004820 218 308 PF01467 Cytidylyltransferase HMMTigr IPR004821 24 89 TIGR00125 Cytidyltransferase-related IPR004821 215 283 TIGR00125 Cytidyltransferase-related
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002852 389 602679 100.0 100.0 NP_001269133 357 602679 100.0 91.8 XP_016880400 357 602679 100.0 91.8 NP_001317447 367 602679 94.3 100.0 NP_001243362 335 602679 85.9 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5546 Download

>KIEE5546  1167 bp
ATGATCCGGAACGGGCGCGGGGCTGCAGGCGGCGCAGAGCAGCCGGGCCCGGGGGGCAGG
CGCGCCGTGAGGGTGTGGTGCGATGGCTGCTATGACATGGTGCATTACGGCCACTCCAAC
CAGCTGCGCCAGGCACGGGCCATGGGTGACTACCTCATCGTAGGCGTGCACACCGATGAG
GAGATCGCCAAGCACAAGGGGCCCCCGGTGTTCACTCAGGAGGAGAGATACAAGATGGTG
CAGGCCATCAAATGGGTGGACGAGGTGGTGCCAGCGGCTCCCTACGTCACTACACTAGAG
ACCCTGGACAAATACAACTGTGACTTCTGTGTTCACGGCAATGACATCACCCTGACTGTA
GATGGCCGGGACACCTATGAGGAAGTAAAGCAGGCTGGGAGGTACAGAGAATGCAAGCGC
ACGCAAGGGGTGTCCACCACAGACCTCGTGGGCCGCATGCTGCTGGTAACCAAAGCCCAT
CACAGCAGCCAGGAGATGTCCTCTGAGTACCGGGAGTATGCAGACAGTTTTGGCAAGTGC
CCTGGTGGGCGGAACCCCTGGACCGGGGTATCCCAGTTCCTGCAGACATCTCAGAAGATC
ATCCAGTTTGCTTCTGGGAAGGAGCCCCAGCCAGGGGAGACAGTCATCTATGTGGCTGGT
GCCTTCGACCTGTTCCACATCGGGCATGTGGACTTCCTGGAGAAGGTGCACAGGCTGGCA
GAGAGGCCCTACATCATCGCGGGCTTACACTTTGACCAGGAGGTCAATCACTACAAGGGG
AAGAACTACCCCATCATGAATCTGCATGAACGGACTCTGAGCGTGCTGGCCTGCCGGTAC
GTGTCAGAAGTGGTGATTGGAGCCCCGTACGCGGTCACAGCAGAGCTCCTAAGTCACTTC
AAGGTGGACCTGGTGTGTCACGGCAAGACAGAAATTATCCCTGACAGGGATGGCTCCGAC
CCATACCAGGAGCCCAAGAGAAGGGGCATCTTCCGTCAGATTGACAGTGGCAGCAACCTC
ACCACAGACCTCATCGTCCAGCGGATCATCACCAACAGGTTGGAGTATGAGGCGCGAAAC
CAGAAGAAGGAAGCCAAGGAGCTGGCCTTCCTGGAGGCTGCCAGGCAGCAGGCGGCACAG
CCCCTGGGGGAGCGCGATGGTGACTTC

ORF Protein

Cloned ORF protein sequence for pFN21AE5546 Download

>KIEE5546  389 aa
MIRNGRGAAGGAEQPGPGGRRAVRVWCDGCYDMVHYGHSNQLRQARAMGDYLIVGVHTDE
EIAKHKGPPVFTQEERYKMVQAIKWVDEVVPAAPYVTTLETLDKYNCDFCVHGNDITLTV
DGRDTYEEVKQAGRYRECKRTQGVSTTDLVGRMLLVTKAHHSSQEMSSEYREYADSFGKC
PGGRNPWTGVSQFLQTSQKIIQFASGKEPQPGETVIYVAGAFDLFHIGHVDFLEKVHRLA
ERPYIIAGLHFDQEVNHYKGKNYPIMNLHERTLSVLACRYVSEVVIGAPYAVTAELLSHF
KVDLVCHGKTEIIPDRDGSDPYQEPKRRGIFRQIDSGSNLTTDLIVQRIITNRLEYEARN
QKKEAKELAFLEAARQQAAQPLGERDGDF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5546 Download

>pFN21AE5546  5872 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGATCCGGAACGGGCGCGGGGCTGCAGGCGGCGC
AGAGCAGCCGGGCCCGGGGGGCAGGCGCGCCGTGAGGGTGTGGTGCGATGGCTGCTATGA
CATGGTGCATTACGGCCACTCCAACCAGCTGCGCCAGGCACGGGCCATGGGTGACTACCT
CATCGTAGGCGTGCACACCGATGAGGAGATCGCCAAGCACAAGGGGCCCCCGGTGTTCAC
TCAGGAGGAGAGATACAAGATGGTGCAGGCCATCAAATGGGTGGACGAGGTGGTGCCAGC
GGCTCCCTACGTCACTACACTAGAGACCCTGGACAAATACAACTGTGACTTCTGTGTTCA
CGGCAATGACATCACCCTGACTGTAGATGGCCGGGACACCTATGAGGAAGTAAAGCAGGC
TGGGAGGTACAGAGAATGCAAGCGCACGCAAGGGGTGTCCACCACAGACCTCGTGGGCCG
CATGCTGCTGGTAACCAAAGCCCATCACAGCAGCCAGGAGATGTCCTCTGAGTACCGGGA
GTATGCAGACAGTTTTGGCAAGTGCCCTGGTGGGCGGAACCCCTGGACCGGGGTATCCCA
GTTCCTGCAGACATCTCAGAAGATCATCCAGTTTGCTTCTGGGAAGGAGCCCCAGCCAGG
GGAGACAGTCATCTATGTGGCTGGTGCCTTCGACCTGTTCCACATCGGGCATGTGGACTT
CCTGGAGAAGGTGCACAGGCTGGCAGAGAGGCCCTACATCATCGCGGGCTTACACTTTGA
CCAGGAGGTCAATCACTACAAGGGGAAGAACTACCCCATCATGAATCTGCATGAACGGAC
TCTGAGCGTGCTGGCCTGCCGGTACGTGTCAGAAGTGGTGATTGGAGCCCCGTACGCGGT
CACAGCAGAGCTCCTAAGTCACTTCAAGGTGGACCTGGTGTGTCACGGCAAGACAGAAAT
TATCCCTGACAGGGATGGCTCCGACCCATACCAGGAGCCCAAGAGAAGGGGCATCTTCCG
TCAGATTGACAGTGGCAGCAACCTCACCACAGACCTCATCGTCCAGCGGATCATCACCAA
CAGGTTGGAGTATGAGGCGCGAAACCAGAAGAAGGAAGCCAAGGAGCTGGCCTTCCTGGA
GGCTGCCAGGCAGCAGGCGGCACAGCCCCTGGGGGAGCGCGATGGTGACTTCGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT