################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AgeI AhdI AocI Aor51HI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsiEI BsmBI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrGI Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MscI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 5 TCCGGA T^CCGGA AflIII 839 ACGTGT A^C[GA][CT]GT AscI 59 GGCGCGCC GG^CGCGCC AvaI 48 CCCGGG C^[CT]CG[GA]G BciVI 568 GTATCC GTATCC(6/5) BfaI 295 CTAG C^TAG BglI 47 GCCCGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 838 TACGTG [CT]AC^GT[GA] BsaI 298 GAGACC GGTCTC(1/5) BsaWI 5 TCCGGA [AT]^CCGG[AT] BseAI 5 TCCGGA T^CCGGA BsgI 238 GTGCAG GTGCAG(16/14) BsiWI 866 CGTACG C^GTACG BsmFI 368 GGGAC GGGAC(10/14) BsmI 409 GAATGC GAATGC(1/-1) BsoBI 48 CCCGGG C^[CT]CG[GA]G BspEI 5 TCCGGA T^CCGGA BspHI 794 TCATGA T^CATGA BsrDI 338 GCAATG GCAATG(2/0) BsrFI 833 GCCGGT [GA]^CCGG[CT] BssHII 60 GCGCGC G^CGCGC BssSI 445 CTCGTG CACGAG(-5/-1) BstEII 465 GGTAACC G^GT[ACGT]ACC BstPI 465 GGTAACC G^GT[ACGT]ACC Cfr10I 833 GCCGGT [GA]^CCGG[CT] DsaI 140 CCATGG C^C[GA][CT]GG EcoNI 696 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 465 GGTAACC G^GT[ACGT]ACC MaeI 295 CTAG C^TAG MroI 5 TCCGGA T^CCGGA NcoI 140 CCATGG C^CATGG PflMI 245 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 813 GACTC GAGTC(4/5) PvuII 121 CAGCTG CAG^CTG RcaI 794 TCATGA T^CATGA SacI 883 GAGCTC GAGCT^C SexAI 908 ACCTGGT A^CC[AT]GGT SfaNI 986 GCATC GCATC(5/9) SmaI 48 CCCGGG CCC^GGG SphI 455 GCATGC GCATG^C TaqI 665 TCGA T^CGA TfiI 798 GAATC G^A[AT]TC Tsp509I 933 AATT ^AATT TspRI 1005 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 665 TCGA T^CGA Van91I 245 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 48 CCCGGG C^CCGGG XmaI 48 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Alw44I 166 GTGCAC G^TGCAC 706 GTGCAC AlwI 3 GATCC GGATC(4/5) 1043 GGATC AlwNI 574 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG 1138 CAGCCCCTG ApaI 45 GGGCCC GGGCC^C 199 GGGCCC ApaLI 166 GTGCAC G^TGCAC 706 GTGCAC AvaII 560 GGACC G^G[AT]CC 906 GGACC BanI 267 GGTGCC G^G[CT][GA]CC 658 GGTGCC BpmI 697 CTGGAG CTGGAG(16/14) 1111 CTGGAG BseRI 178 GAGGAG GAGGAG(10/8) 220 GAGGAG BsmAI 298 GAGAC GTCTC(1/5) 637 GAGAC BsrI 573 CCAGT ACTGG(1/-1) 603 CCAGT BstF5I 600 CATCC GGATG(2/0) 948 GGATG BstXI 1052 CCAACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1094 CCAAGGAGCTGG DrdI 754 GACCAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1027 GACCTCATCGTC EaeI 108 CGGCCA [CT]^GGCC[GA] 363 TGGCCG EcoT14I 140 CCATGG C^C[AT][AT]GG 1094 CCAAGG FauI 17 GCGGG CCCGC(4/6) 739 GCGGG FokI 600 CATCC GGATG(9/13) 948 GGATG HinfI 798 GAATC G^A[ACGT]TC 813 GACTC MaeII 284 ACGT A^CGT 839 ACGT NspI 455 GCATGC [GA]CATG^[CT] 684 GCATGT PstI 23 CTGCAG CTGCA^G 580 CTGCAG StyI 140 CCATGG C^C[AT][AT]GG 1094 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AfaI AluI AspHI BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI TseI Tsp45I XcmI