KCNK6 ORF clone pFN21AE5401

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Halotag ORF Clone pFN21AE5401

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Clone info.

Species

human

Clone Name

pFN21AE5401 Sequence (with vector) Restriction Map

Product ID

FHC08183

ORF Sequence

DNA sequence (939 bp)
Protein sequence (313 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5401

Symbol

KCNK6

Alias : K2p6.1, KCNK8, TOSS, TWIK-2, TWIK2

Description

potassium channel, two pore domain subfamily K, member 6

Original Clone ID

oc95401

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 939 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 313 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12513.1 9.2e-133 100.0 KCNK6 CCDS1599.1 2.2e-48 45.5 KCNK1 CCDS8067.1 5e-28 35.8 KCNK4 CCDS31608.1 2.3e-27 36.4 KCNK7 CCDS4842.1 4.7e-26 33.0 KCNK17 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR005408 53 65 PR01586 Potassium channel IPR005408 67 83 PR01586 Potassium channel IPR003280 102 130 PR01333 Potassium channel IPR005408 132 144 PR01586 Potassium channel IPR005409 153 167 PR01587 Potassium channel IPR005409 171 181 PR01587 Potassium channel IPR003280 213 222 PR01333 Potassium channel IPR005408 222 235 PR01586 Potassium channel IPR005408 239 252 PR01586 Potassium channel IPR005409 241 253 PR01587 Potassium channel IPR005408 254 272 PR01586 Potassium channel IPR005409 255 271 PR01587 Potassium channel IPR005409 282 295 PR01587 Potassium channel HMMPfam IPR013099 89 145 PF07885 Ion transport 2 IPR013099 181 257 PF07885 Ion transport 2
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 6 LLAGALAAYAAYLVLGALLVARL 28 PRIMARY 23 2 90 AWDFASALFFASTLITTVGYGYT 112 SECONDARY 23 3 119 GKAFSIAFALLGVPTTMLLLTAS 141 SECONDARY 23 4 171 WHLVALLGVVVTVCFLVPAVIFA 193 PRIMARY 23 5 198 AWSFLDAFYFCFISLSTIGLGDY 220 SECONDARY 23 6 233 LYKVLVTVYLFLGLVAMVLVLQT 255 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004814 313 603939 100.0 100.0 NP_002236 336 601745 45.5 98.4 NP_001304019 393 605720 35.8 95.8 NP_201567 393 605720 35.8 95.8 NP_203133 307 603940 36.4 92.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5401 Download

>KIEE5401  939 bp
ATGCGGAGGGGCGCGCTTCTGGCGGGCGCCTTGGCCGCGTACGCCGCGTACCTGGTGCTG
GGCGCGCTGTTGGTGGCGCGGCTGGAGGGGCCGCACGAAGCCAGGCTCCGAGCCGAGCTG
GAGACGCTGCGGGCGCAGCTGCTTCAGCGCAGCCCGTGTGTGGCTGCCCCCGCCCTGGAC
GCCTTCGTGGAGCGAGTGCTGGCGGCCGGACGGCTGGGGCGGGTCGTGCTTGCTAACGCT
TCGGGGTCCGCCAACGCCTCGGACCCCGCCTGGGACTTCGCCTCTGCTCTCTTCTTCGCC
AGCACGCTGATCACCACCGTGGGCTATGGGTACACAACGCCACTGACTGATGCGGGCAAG
GCCTTCTCCATCGCCTTTGCGCTCCTGGGCGTGCCGACCACCATGCTGCTGCTGACCGCC
TCAGCCCAGCGCCTGTCACTGCTGCTGACTCACGTGCCCCTGTCTTGGCTGAGCATGCGT
TGGGGCTGGGACCCCCGGCGGGCGGCCTGCTGGCACTTGGTGGCCCTGTTGGGGGTCGTA
GTGACCGTCTGCTTTCTGGTGCCGGCTGTGATCTTTGCCCACCTCGAGGAGGCCTGGAGC
TTCTTGGATGCCTTCTACTTCTGCTTTATCTCTCTGTCCACCATCGGCCTGGGCGACTAC
GTGCCCGGGGAGGCCCCTGGCCAGCCCTACCGGGCCCTCTACAAGGTGCTGGTCACAGTC
TACCTCTTCCTGGGCCTGGTGGCCATGGTGCTGGTGCTGCAGACCTTCCGCCACGTGTCC
GACCTCCACGGCCTCACGGAGCTCATCCTGCTGCCCCCTCCGTGCCCTGCCAGTTTCAAT
GCGGATGAGGACGATCGGGTGGACATCCTGGGCCCCCAGCCGGAGTCGCACCAGCAACTC
TCTGCCAGCTCCCACACCGACTACGCTTCCATCCCCAGG

ORF Protein

Cloned ORF protein sequence for pFN21AE5401 Download

>KIEE5401  313 aa
MRRGALLAGALAAYAAYLVLGALLVARLEGPHEARLRAELETLRAQLLQRSPCVAAPALD
AFVERVLAAGRLGRVVLANASGSANASDPAWDFASALFFASTLITTVGYGYTTPLTDAGK
AFSIAFALLGVPTTMLLLTASAQRLSLLLTHVPLSWLSMRWGWDPRRAACWHLVALLGVV
VTVCFLVPAVIFAHLEEAWSFLDAFYFCFISLSTIGLGDYVPGEAPGQPYRALYKVLVTV
YLFLGLVAMVLVLQTFRHVSDLHGLTELILLPPPCPASFNADEDDRVDILGPQPESHQQL
SASSHTDYASIPR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5401 Download

>pFN21AE5401  5644 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCGGAGGGGCGCGCTTCTGGCGGGCGCCTTGGC
CGCGTACGCCGCGTACCTGGTGCTGGGCGCGCTGTTGGTGGCGCGGCTGGAGGGGCCGCA
CGAAGCCAGGCTCCGAGCCGAGCTGGAGACGCTGCGGGCGCAGCTGCTTCAGCGCAGCCC
GTGTGTGGCTGCCCCCGCCCTGGACGCCTTCGTGGAGCGAGTGCTGGCGGCCGGACGGCT
GGGGCGGGTCGTGCTTGCTAACGCTTCGGGGTCCGCCAACGCCTCGGACCCCGCCTGGGA
CTTCGCCTCTGCTCTCTTCTTCGCCAGCACGCTGATCACCACCGTGGGCTATGGGTACAC
AACGCCACTGACTGATGCGGGCAAGGCCTTCTCCATCGCCTTTGCGCTCCTGGGCGTGCC
GACCACCATGCTGCTGCTGACCGCCTCAGCCCAGCGCCTGTCACTGCTGCTGACTCACGT
GCCCCTGTCTTGGCTGAGCATGCGTTGGGGCTGGGACCCCCGGCGGGCGGCCTGCTGGCA
CTTGGTGGCCCTGTTGGGGGTCGTAGTGACCGTCTGCTTTCTGGTGCCGGCTGTGATCTT
TGCCCACCTCGAGGAGGCCTGGAGCTTCTTGGATGCCTTCTACTTCTGCTTTATCTCTCT
GTCCACCATCGGCCTGGGCGACTACGTGCCCGGGGAGGCCCCTGGCCAGCCCTACCGGGC
CCTCTACAAGGTGCTGGTCACAGTCTACCTCTTCCTGGGCCTGGTGGCCATGGTGCTGGT
GCTGCAGACCTTCCGCCACGTGTCCGACCTCCACGGCCTCACGGAGCTCATCCTGCTGCC
CCCTCCGTGCCCTGCCAGTTTCAATGCGGATGAGGACGATCGGGTGGACATCCTGGGCCC
CCAGCCGGAGTCGCACCAGCAACTCTCTGCCAGCTCCCACACCGACTACGCTTCCATCCC
CAGGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT