(Circular) (Six-base) MAPSORT of: pFN21AE5401.seq Check: 3910 from: 1 to: 5644 pFN21AE5401 5644 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2724 2987 1937 Size: 787 263 4594 Fragments arranged by size: 4594 787 263 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2132 3707 5091 5359 1632 Size: 500 1575 1384 268 1917 Fragments arranged by size: 1917 1575 1384 500 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 2778 4543 5520 1203 Size: 1575 1765 977 1327 Fragments arranged by size: 1765 1575 1327 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 2321 1166 Size: 1155 4489 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2922 1184 Size: 1738 3906 AlwNI CAG_nnn'CTG Cuts at: 2437 3387 4959 2437 Size: 950 1572 3122 Fragments arranged by size: 3122 1572 950 ApaI G_GGCC'C Cuts at: 2701 2879 2701 Size: 178 5466 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2953 3179 3331 5440 1493 Size: 1460 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2588 2669 2969 1870 Size: 73 645 81 300 4545 Fragments arranged by size: 4545 645 300 81 73 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2030 2563 2965 4420 618 Size: 325 217 627 243 533 402 1455 1842 Fragments arranged by size: 1842 1455 627 533 402 325 243 217 BanII G_rGCy'C Cuts at: 1391 2701 2808 2879 2963 1391 Size: 1310 107 71 84 4072 Fragments arranged by size: 4072 1310 107 84 71 BbeI G_GCGC'C Cuts at: 2034 2034 Size: 5644 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4673 BbvCI CC'TCA_GC Cuts at: 2425 2425 Size: 5644 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2389 2423 3835 3869 1903 Size: 34 50 34 368 34 1412 34 3678 Fragments arranged by size: 3678 1412 368 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 2313 1548 Size: 765 4879 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4255 136 Size: 107 122 71 631 605 2583 1525 Fragments arranged by size: 2583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 2474 3061 2474 Size: 587 5057 Bme1580I G_kGCm'C Cuts at: 1790 2463 2670 2701 2831 2879 3705 4861 1790 Size: 673 207 31 130 48 826 1156 2573 Fragments arranged by size: 2573 1156 826 673 207 130 48 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2144 2620 4304 1239 Size: 869 36 476 1684 2579 Fragments arranged by size: 2579 1684 869 476 36 Bpu10I CC'TnA_GC Cuts at: 2425 2425 Size: 5644 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3769 4634 4932 3769 Size: 865 298 4481 Fragments arranged by size: 4481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4307 882 Size: 617 2808 2219 Fragments arranged by size: 2808 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2458 2665 2779 3435 493 Size: 711 1254 207 114 656 2702 Fragments arranged by size: 2702 1254 711 656 207 114 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 2184 3451 3833 Size: 53 83 186 936 498 153 1267 382 Cuts at: 3833 4468 275 Size: 635 1451 Fragments arranged by size: 1451 1267 936 635 498 382 186 153 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5419 Fragments arranged by size: 5419 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2606 1186 Size: 1420 4224 BseYI C'CCAG_C Cuts at: 2062 2218 2430 2490 2880 4847 5343 2062 Size: 156 212 60 390 1967 496 2363 Fragments arranged by size: 2363 1967 496 390 212 156 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5071 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2211 2860 3094 3855 4883 1540 Size: 275 186 210 649 234 761 1028 2301 Fragments arranged by size: 2301 1028 761 649 275 234 210 186 BsiHKAI G_wGCw'C Cuts at: 2808 3705 3790 4861 2808 Size: 897 85 1071 3591 Fragments arranged by size: 3591 1071 897 85 BsiWI C'GTAC_G Cuts at: 2043 2043 Size: 5644 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 BsmBI CGTCTCn'nnnn_ Cuts at: 2120 2120 Size: 5644 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2463 2670 2701 2808 2831 2879 2963 Size: 399 673 207 31 107 23 48 84 Cuts at: 2963 3705 3790 4861 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 673 399 207 107 85 84 48 31 23 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2999 844 Size: 2155 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2566 4288 4475 1700 Size: 110 756 1722 187 2869 Fragments arranged by size: 2869 1722 756 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 2016 2067 1925 Size: 91 51 5502 Fragments arranged by size: 5502 91 51 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstXI CCAn_nnnn'nTGG Cuts at: 2653 2755 2653 Size: 102 5542 BstYI r'GATC_y Cuts at: 1972 2974 3341 3726 3743 5195 5281 5293 5639 Size: 1002 367 385 17 1452 86 12 346 Cuts at: 5639 1972 Size: 1977 Fragments arranged by size: 1977 1452 1002 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2322 2748 2791 513 Size: 552 1257 426 43 3366 Fragments arranged by size: 3366 1257 552 426 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2359 487 Size: 683 56 1133 3772 Fragments arranged by size: 3772 1133 683 56 BtsI GCAGTG_nn' Cuts at: 2441 3160 3954 3974 2441 Size: 719 794 20 4111 Fragments arranged by size: 4111 794 719 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DraIII CAC_nnn'GTG Cuts at: 2524 2524 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2208 2683 Size: 54 1425 50 275 18 207 171 475 Cuts at: 2683 2745 3091 3980 5609 8 Size: 62 346 889 1629 43 Fragments arranged by size: 1629 1425 889 475 346 275 207 171 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2208 3091 1537 Size: 275 396 883 4090 Fragments arranged by size: 4090 883 396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2300 2735 3574 1353 Size: 947 435 839 3423 Fragments arranged by size: 3423 947 839 435 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2242 2762 4231 4605 4751 387 Size: 1064 138 653 520 1469 374 146 1280 Fragments arranged by size: 1469 1280 1064 653 520 374 146 138 EcoICRI GAG'CTC Cuts at: 2806 2806 Size: 5644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2132 2144 2620 3707 4304 5091 Size: 393 476 24 12 476 1087 597 787 Cuts at: 5091 5359 1239 Size: 268 1524 Fragments arranged by size: 1524 1087 787 597 476 476 393 268 24 12 EcoNI CCTnn'n_nnAGG Cuts at: 2591 2705 2591 Size: 114 5530 EcoO109I rG'GnC_Cy Cuts at: 2494 2677 2698 2876 2494 Size: 183 21 178 5262 Fragments arranged by size: 5262 183 178 21 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2034 2437 4791 1678 Size: 356 403 2354 2531 Fragments arranged by size: 2531 2354 403 356 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2270 2302 4285 4317 4359 Size: 32 135 32 698 32 1983 32 42 Cuts at: 4359 4391 1373 Size: 32 2626 Fragments arranged by size: 2626 1983 698 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2988 3234 669 Size: 988 281 1050 246 3079 Fragments arranged by size: 3079 1050 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KasI G'GCGC_C Cuts at: 2030 2030 Size: 5644 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2808 4758 4942 1305 Size: 1503 1950 184 2007 Fragments arranged by size: 2007 1950 1503 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2685 2747 10 Size: 54 1425 343 853 62 2907 Fragments arranged by size: 2907 1425 853 343 62 54 MslI CAynn'nnrTG Cuts at: 518 1166 2321 3602 3961 4120 518 Size: 648 1155 1281 359 159 2042 Fragments arranged by size: 2042 1281 1155 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2143 3060 3737 4885 5130 1606 Size: 537 917 677 1148 245 2120 Fragments arranged by size: 2120 1148 917 677 537 245 NaeI GCC'GGC Cuts at: 2568 2568 Size: 5644 NarI GG'CG_CC Cuts at: 2031 2031 Size: 5644 NcoI C'CATG_G Cuts at: 513 1065 2748 513 Size: 552 1683 3409 Fragments arranged by size: 3409 1683 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NgoMIV G'CCGG_C Cuts at: 2566 2566 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 2482 3002 4547 1132 Size: 97 1253 520 1545 2229 Fragments arranged by size: 2229 1545 1253 520 97 PasI CC'CwG_GG Cuts at: 1348 2940 1348 Size: 1592 4052 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PmlI CAC'GTG Cuts at: 2458 2779 2458 Size: 321 5323 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2494 2494 Size: 5644 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PspOMI G'GGCC_C Cuts at: 2697 2875 2697 Size: 178 5466 PspXI vC'TCGA_Gb Cuts at: 2588 2588 Size: 5644 PstI C_TGCA'G Cuts at: 830 2766 2996 830 Size: 1936 230 3478 Fragments arranged by size: 3478 1936 230 PvuI CG_AT'CG Cuts at: 2001 2860 2001 Size: 859 4785 PvuII CAG'CTG Cuts at: 2143 2143 Size: 5644 SacI G_AGCT'C Cuts at: 2808 2808 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SanDI GG'GwC_CC Cuts at: 2494 2494 Size: 5644 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SexAI A'CCwGG_T Cuts at: 2055 2055 Size: 5644 SfcI C'TryA_G Cuts at: 826 1045 2762 2992 4127 4808 4999 826 Size: 219 1717 230 1135 681 191 1471 Fragments arranged by size: 1717 1471 1135 681 230 219 191 SfoI GGC'GCC Cuts at: 2032 2032 Size: 5644 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2671 2971 1872 Size: 799 300 4545 Fragments arranged by size: 4545 799 300 SmlI C'TyrA_G Cuts at: 820 1017 1943 2588 3748 4649 4911 820 Size: 197 926 645 1160 901 262 1553 Fragments arranged by size: 1553 1160 926 901 645 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 2482 3002 1132 Size: 1350 520 3774 Fragments arranged by size: 3774 1350 520 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 2366 2597 1517 Size: 849 231 4564 Fragments arranged by size: 4564 849 231 StyI C'CwwG_G Cuts at: 513 1065 2034 2748 3083 513 Size: 552 969 714 335 3074 Fragments arranged by size: 3074 969 714 552 335 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3671 3688 3841 4026 4471 325 Size: 1255 2091 17 153 185 445 1498 Fragments arranged by size: 2091 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3890 5602 96 Size: 274 80 33 51 1443 1913 1712 138 Fragments arranged by size: 1913 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2650 2650 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 2588 1943 Size: 645 4999 XmaI C'CCGG_G Cuts at: 1870 2669 2969 1870 Size: 799 300 4545 Fragments arranged by size: 4545 799 300 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PsrI RsrII SacII SapI SfiI SrfI SwaI