EIF2B5 ORF clone pFN21AE4568

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Halotag ORF Clone pFN21AE4568

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Clone info.

Species

human

Clone Name

pFN21AE4568 Sequence (with vector) Restriction Map

Product ID

FHC08700

ORF Sequence

DNA sequence (2163 bp)
Protein sequence (721 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4568

Symbol

EIF2B5

Alias : CACH, CLE, EIF-2B, EIF2Bepsilon, LVWM

Description

eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa

Original Clone ID

oc94568

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2163 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 721 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3252.1 0 99.9 EIF2B5 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003307 641 721 PF02020 eIF4-gamma/eIF5/eIF2-epsilon HMMSmart IPR003307 629 716 SM00515 eIF4-gamma/eIF5/eIF2-epsilon ProfileScan IPR003307 543 720 PS51363 eIF4-gamma/eIF5/eIF2-epsilon
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_003898 721 603896,603945 99.9 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4568 Download

>KIEE4568  2163 bp
ATGGCGGCCCCTGTAGTGGCGCCGCCTGGTGTGGTGGTTAGTCGGGCTAACAAGCGCAGC
GGCGCGGGGCCGGGAGGCAGCGGTGGCGGGGGAGCCAGAGGGGCGGAGGAGGAACCGCCG
CCGCCCCTACAAGCAGTTCTGGTGGCCGATAGCTTCGATCGCCGCTTCTTCCCCATCTCC
AAGGACCAGCCTCGGGTCCTCTTGCCCCTGGCCAATGTGGCATTAATTGACTACACTCTG
GAATTCCTGACTGCCACAGGTGTACAGGAAACATTTGTCTTTTGTTGCTGGAAAGCTGCT
CAAATCAAAGAACATTTACTGAAGTCAAAGTGGTGCCGCCCTACATCTCTCAATGTGGTT
CGAATAATTACATCAGAGCTCTATCGATCACTGGGAGATGTCCTCCGTGATGTTGATGCC
AAGGCTTTGGTGCGCTCTGACTTTCTTCTGGTGTATGGGGATGTCATCTCAAACATCAAT
ATCACCAGAGCCCTTGAGGAACACAGGTTGAGACGGAAGCTAGAAAAAAATGTTTCTGTG
ATGACGATGATCTTCAAGGAGTCATCCCCCAGCCACCCAACTCGTTGCCACGAAGACAAT
GTGGTAGTGGCTGTGGATAGTACCACAAACAGGGTTCTCCATTTTCAGAAGACCCAGGGT
CTCCGGCGTTTTGCATTTCCTCTGAGCCTGTTTCAGGGCAGTAGTGATGGAGTGGAGGTT
CGATATGATTTACTGGATTGTCATATCAGCATCTGTTCTCCTCAGGTGGCACAACTCTTT
ACAGACAACTTTGACTACCAAACTCGAGATGACTTTGTGCGAGGTCTCTTAGTGAATGAG
GAGATCCTAGGGAACCAGATCCACATGCACGTAACAGCTAAGGAATATGGTGCCCGTGTC
TCCAACCTACACATGTACTCAGCTGTCTGTGCTGACGTCATCCGCCGATGGGTCTACCCT
CTCACCCCAGAGGCGAACTTCACTGACAGCACCACCCAGAGCTGCACTCATTCCCGGCAC
AACATCTACCGAGGGCCTGAGGTCAGCCTGGGCCATGGCAGCATCCTAGAGGAAAATGTG
CTCCTGGGCTCTGGCACTGTCATTGGCAGCAATTGCTTTATCACCAACAGTGTCATTGGC
CCCGGCTGCCACATTGGTGATAACGTGGTGCTGGACCAGACCTACCTGTGGCAGGGTGTT
CGAGTGGCGGCTGGAGCACAGATCCATCAGTCTCTGCTTTGTGACAATGCTGAGGTCAAG
GAACGAGTGACACTGAAACCACGCTCTGTCCTCACTTCCCAGGTGGTCGTGGGCCCAAAT
ATCACGCTGCCTGAGGGCTCGGTGATCTCTTTGCACCCTCCAGATGCAGAGGAAGATGAA
GATGATGGCGAGTTCAGTGATGATTCTGGGGCTGACCAAGAAAAGGACAAAGTGAAGATG
AAAGGTTACAATCCAGCAGAAGTAGGAGCTGCTGGCAAGGGCTACCTCTGGAAAGCTGCA
GGCATGAACATGGAGGAAGAGGAGGAACTGCAGCAGAATCTGTGGGGACTCAAGATCAAC
ATGGAAGAAGAGAGTGAAAGTGAAAGTGAGCAAAGTATGGATTCTGAGGAGCCGGACAGC
CGGGGAGGCTCCCCTCAGATGGATGACATCAAAGTGTTCCAGAATGAAGTTTTAGGAACA
CTACAGCGGGGCAAAGAGGAGAACATTTCTTGTGACAATCTCGTCCTGGAAATCAACTCT
CTCAAGTATGCCTATAACGTAAGTCTAAAGGAGGTGATGCAGGTACTGAGCCACGTGGTC
CTGGAGTTCCCCCTGCAACAGATGGATTCCCCGCTTGACTCAAGCCGCTACTGTGCCCTG
CTGCTTCCTCTGCTAAAGGCCTGGAGCCCTGTTTTTAGGAACTACATAAAGCGCGCAGCC
GACCATTTGGAAGCGTTAGCAGCCATTGAGGACTTCTTCCTAGAGCATGAAGCTCTTGGT
ATTTCCATGGCCAAGGTACTGATGGCTTTCTACCAGCTGGAGATCCTGGCTGAGGAAACA
ATTCTGAGCTGGTTCAGCCAAAGAGATACAACTGACAAGGGCCAGCAGTTGCGCAAGAAT
CAACAGCTGCAGAGGTTCATCCAGTGGCTAAAAGAGGCAGAAGAGGAGTCATCTGAAGAT
GAC

ORF Protein

Cloned ORF protein sequence for pFN21AE4568 Download

>KIEE4568  721 aa
MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPIS
KDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLLKSKWCRPTSLNVV
RIITSELYRSLGDVLRDVDAKALVRSDFLLVYGDVISNINITRALEEHRLRRKLEKNVSV
MTMIFKESSPSHPTRCHEDNVVVAVDSTTNRVLHFQKTQGLRRFAFPLSLFQGSSDGVEV
RYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEEILGNQIHMHVTAKEYGARV
SNLHMYSAVCADVIRRWVYPLTPEANFTDSTTQSCTHSRHNIYRGPEVSLGHGSILEENV
LLGSGTVIGSNCFITNSVIGPGCHIGDNVVLDQTYLWQGVRVAAGAQIHQSLLCDNAEVK
ERVTLKPRSVLTSQVVVGPNITLPEGSVISLHPPDAEEDEDDGEFSDDSGADQEKDKVKM
KGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDS
RGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNVSLKEVMQVLSHVV
LEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALG
ISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLKEAEEESSED
D

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4568 Download

>pFN21AE4568  6868 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGGCCCCTGTAGTGGCGCCGCCTGGTGTGGT
GGTTAGTCGGGCTAACAAGCGCAGCGGCGCGGGGCCGGGAGGCAGCGGTGGCGGGGGAGC
CAGAGGGGCGGAGGAGGAACCGCCGCCGCCCCTACAAGCAGTTCTGGTGGCCGATAGCTT
CGATCGCCGCTTCTTCCCCATCTCCAAGGACCAGCCTCGGGTCCTCTTGCCCCTGGCCAA
TGTGGCATTAATTGACTACACTCTGGAATTCCTGACTGCCACAGGTGTACAGGAAACATT
TGTCTTTTGTTGCTGGAAAGCTGCTCAAATCAAAGAACATTTACTGAAGTCAAAGTGGTG
CCGCCCTACATCTCTCAATGTGGTTCGAATAATTACATCAGAGCTCTATCGATCACTGGG
AGATGTCCTCCGTGATGTTGATGCCAAGGCTTTGGTGCGCTCTGACTTTCTTCTGGTGTA
TGGGGATGTCATCTCAAACATCAATATCACCAGAGCCCTTGAGGAACACAGGTTGAGACG
GAAGCTAGAAAAAAATGTTTCTGTGATGACGATGATCTTCAAGGAGTCATCCCCCAGCCA
CCCAACTCGTTGCCACGAAGACAATGTGGTAGTGGCTGTGGATAGTACCACAAACAGGGT
TCTCCATTTTCAGAAGACCCAGGGTCTCCGGCGTTTTGCATTTCCTCTGAGCCTGTTTCA
GGGCAGTAGTGATGGAGTGGAGGTTCGATATGATTTACTGGATTGTCATATCAGCATCTG
TTCTCCTCAGGTGGCACAACTCTTTACAGACAACTTTGACTACCAAACTCGAGATGACTT
TGTGCGAGGTCTCTTAGTGAATGAGGAGATCCTAGGGAACCAGATCCACATGCACGTAAC
AGCTAAGGAATATGGTGCCCGTGTCTCCAACCTACACATGTACTCAGCTGTCTGTGCTGA
CGTCATCCGCCGATGGGTCTACCCTCTCACCCCAGAGGCGAACTTCACTGACAGCACCAC
CCAGAGCTGCACTCATTCCCGGCACAACATCTACCGAGGGCCTGAGGTCAGCCTGGGCCA
TGGCAGCATCCTAGAGGAAAATGTGCTCCTGGGCTCTGGCACTGTCATTGGCAGCAATTG
CTTTATCACCAACAGTGTCATTGGCCCCGGCTGCCACATTGGTGATAACGTGGTGCTGGA
CCAGACCTACCTGTGGCAGGGTGTTCGAGTGGCGGCTGGAGCACAGATCCATCAGTCTCT
GCTTTGTGACAATGCTGAGGTCAAGGAACGAGTGACACTGAAACCACGCTCTGTCCTCAC
TTCCCAGGTGGTCGTGGGCCCAAATATCACGCTGCCTGAGGGCTCGGTGATCTCTTTGCA
CCCTCCAGATGCAGAGGAAGATGAAGATGATGGCGAGTTCAGTGATGATTCTGGGGCTGA
CCAAGAAAAGGACAAAGTGAAGATGAAAGGTTACAATCCAGCAGAAGTAGGAGCTGCTGG
CAAGGGCTACCTCTGGAAAGCTGCAGGCATGAACATGGAGGAAGAGGAGGAACTGCAGCA
GAATCTGTGGGGACTCAAGATCAACATGGAAGAAGAGAGTGAAAGTGAAAGTGAGCAAAG
TATGGATTCTGAGGAGCCGGACAGCCGGGGAGGCTCCCCTCAGATGGATGACATCAAAGT
GTTCCAGAATGAAGTTTTAGGAACACTACAGCGGGGCAAAGAGGAGAACATTTCTTGTGA
CAATCTCGTCCTGGAAATCAACTCTCTCAAGTATGCCTATAACGTAAGTCTAAAGGAGGT
GATGCAGGTACTGAGCCACGTGGTCCTGGAGTTCCCCCTGCAACAGATGGATTCCCCGCT
TGACTCAAGCCGCTACTGTGCCCTGCTGCTTCCTCTGCTAAAGGCCTGGAGCCCTGTTTT
TAGGAACTACATAAAGCGCGCAGCCGACCATTTGGAAGCGTTAGCAGCCATTGAGGACTT
CTTCCTAGAGCATGAAGCTCTTGGTATTTCCATGGCCAAGGTACTGATGGCTTTCTACCA
GCTGGAGATCCTGGCTGAGGAAACAATTCTGAGCTGGTTCAGCCAAAGAGATACAACTGA
CAAGGGCCAGCAGTTGCGCAAGAATCAACAGCTGCAGAGGTTCATCCAGTGGCTAAAAGA
GGCAGAAGAGGAGTCATCTGAAGATGACGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT