(Linear) (Six-base) MAPSORT of: KIEE4568.seq Check: 2506 from: 1 to: 2163 KIEE4568.orf With 182 enzymes: SgfI * March 22, 2012 18:24 .. AatII G_ACGT'C Cuts at: 0 938 2163 Size: 938 1225 AccI GT'mk_AC Cuts at: 0 953 2163 Size: 953 1210 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 2163 Size: 340 1823 AflIII A'CryG_T Cuts at: 0 911 2163 Size: 911 1252 AhdI GACnn_n'nnGTC Cuts at: 0 656 2163 Size: 656 1507 AleI CACnn'nnGTG Cuts at: 0 1154 2163 Size: 1154 1009 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 701 733 2163 Size: 701 32 1430 Fragments arranged by size: 1430 701 32 AlwNI CAG_nnn'CTG Cuts at: 0 752 1233 1539 1785 2163 Size: 752 481 306 246 378 Fragments arranged by size: 752 481 378 306 246 ApaI G_GGCC'C Cuts at: 0 1315 2163 Size: 1315 848 ApoI r'AATT_y Cuts at: 0 241 2163 Size: 241 1922 AseI AT'TA_AT Cuts at: 0 223 2163 Size: 223 1940 AvaI C'yCGr_G Cuts at: 0 191 803 2163 Size: 191 612 1360 Fragments arranged by size: 1360 612 191 AvrII C'CTAG_G Cuts at: 0 846 2163 Size: 846 1317 BanI G'GyrC_C Cuts at: 0 18 332 889 2163 Size: 18 314 557 1274 Fragments arranged by size: 1274 557 314 18 BanII G_rGCy'C Cuts at: 0 380 492 1090 1315 1339 1888 2163 Size: 380 112 598 225 24 549 275 Fragments arranged by size: 598 549 380 275 225 112 24 BbeI G_GCGC'C Cuts at: 0 22 2163 Size: 22 2141 BbsI GAAGACnn'nnnn_ Cuts at: 0 599 655 2163 Size: 599 56 1508 Fragments arranged by size: 1508 599 56 BbvCI CC'TCA_GC Cuts at: 0 1250 2030 2163 Size: 1250 780 133 Fragments arranged by size: 1250 780 133 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1180 1214 2163 Size: 1180 34 949 Fragments arranged by size: 1180 949 34 BglI GCCn_nnn'nGGC Cuts at: 0 100 217 1625 2163 Size: 100 117 1408 538 Fragments arranged by size: 1408 538 117 100 Bme1580I G_kGCm'C Cuts at: 0 894 1315 1857 2163 Size: 894 421 542 306 Fragments arranged by size: 894 542 421 306 BmrI ACTGGGnnnn_n' Cuts at: 0 400 2163 Size: 400 1763 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 418 1022 2163 Size: 418 604 1141 Fragments arranged by size: 1141 604 418 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1232 1343 1822 1902 2038 2163 Size: 1232 111 479 80 136 125 Fragments arranged by size: 1232 479 136 125 111 80 Bpu10I CC'TnA_GC Cuts at: 0 878 1250 2030 2163 Size: 878 372 780 133 Fragments arranged by size: 878 780 372 133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 514 1534 1726 1824 2163 Size: 514 1020 192 98 339 Fragments arranged by size: 1020 514 339 192 98 BsaI GGTCTCn'nnnn_ Cuts at: 0 664 829 2163 Size: 664 165 1334 Fragments arranged by size: 1334 664 165 BsaAI yAC'GTr Cuts at: 0 870 1794 2163 Size: 870 924 369 Fragments arranged by size: 924 870 369 BsaHI Gr'CG_yC Cuts at: 0 19 935 2163 Size: 19 916 1228 Fragments arranged by size: 1228 916 19 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 701 731 2163 Size: 701 30 1432 Fragments arranged by size: 1432 701 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 121 749 853 1534 1621 1711 2158 2163 Size: 121 628 104 681 87 90 447 5 Fragments arranged by size: 681 628 447 121 104 90 87 5 BseYI C'CCAG_C Cuts at: 0 568 2163 Size: 568 1595 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 987 2163 Size: 987 1176 BsiEI CG_ry'CG Cuts at: 0 159 2163 Size: 159 2004 BsiHKAI G_wGCw'C Cuts at: 0 380 1082 1218 2163 Size: 380 702 136 945 Fragments arranged by size: 945 702 380 136 BsmBI CGTCTCn'nnnn_ Cuts at: 0 504 2163 Size: 504 1659 Bsp1286I G_dGCh'C Cuts at: 0 380 492 894 1082 1090 1218 1315 1339 Size: 380 112 402 188 8 128 97 24 Cuts at: 1339 1857 1888 2163 Size: 518 31 275 Fragments arranged by size: 518 402 380 275 188 128 112 97 31 24 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1770 2163 Size: 1770 393 BsrGI T'GTAC_A Cuts at: 0 261 2163 Size: 261 1902 BssHII G'CGCG_C Cuts at: 0 1911 2163 Size: 1911 252 BstAPI GCAn_nnn'nTGC Cuts at: 0 1112 2163 Size: 1112 1051 BstBI TT'CG_AA Cuts at: 0 360 2163 Size: 360 1803 BstXI CCAn_nnnn'nTGG Cuts at: 0 426 2163 Size: 426 1737 BstYI r'GATC_y Cuts at: 0 842 857 1220 2021 2163 Size: 842 15 363 801 142 Fragments arranged by size: 842 801 363 142 15 Bsu36I CC'TnA_GG Cuts at: 0 761 1037 1331 2163 Size: 761 276 294 832 Fragments arranged by size: 832 761 294 276 BtgI C'CryG_G Cuts at: 0 1053 1985 2163 Size: 1053 932 178 Fragments arranged by size: 1053 932 178 ClaI AT'CG_AT Cuts at: 0 384 2163 Size: 384 1779 DrdI GACnn_nn'nnGTC Cuts at: 0 1720 2163 Size: 1720 443 EaeI y'GGCC_r Cuts at: 0 143 209 1988 2163 Size: 143 66 1779 175 Fragments arranged by size: 1779 175 143 66 EarI CTCTTCn'nnn_ Cuts at: 0 1511 1562 2135 2163 Size: 1511 51 573 28 Fragments arranged by size: 1511 573 51 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 118 931 2163 Size: 118 813 1232 Fragments arranged by size: 1232 813 118 EcoICRI GAG'CTC Cuts at: 0 378 2163 Size: 378 1785 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 1232 1343 1822 1902 2038 2163 Size: 340 892 111 479 80 136 125 Fragments arranged by size: 892 479 340 136 125 111 80 EcoNI CCTnn'n_nnAGG Cuts at: 0 691 1189 2029 2163 Size: 691 498 840 134 Fragments arranged by size: 840 691 498 134 EcoO109I rG'GnC_Cy Cuts at: 0 195 1033 2163 Size: 195 838 1130 Fragments arranged by size: 1130 838 195 EcoRI G'AATT_C Cuts at: 0 241 2163 Size: 241 1922 FspI TGC'GCA Cuts at: 0 2092 2163 Size: 2092 71 HaeII r_GCGC'y Cuts at: 0 22 2163 Size: 22 2141 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 451 483 642 674 1205 1237 1538 1570 Size: 451 32 159 32 531 32 301 32 Cuts at: 1570 2134 2163 Size: 564 29 Fragments arranged by size: 564 531 451 301 159 32 32 32 32 29 KasI G'GCGC_C Cuts at: 0 18 2163 Size: 18 2145 MfeI C'AATT_G Cuts at: 0 1110 2163 Size: 1110 1053 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 926 2163 Size: 926 1237 MscI TGG'CCA Cuts at: 0 211 1990 2163 Size: 211 1779 173 Fragments arranged by size: 1779 211 173 MslI CAynn'nnrTG Cuts at: 0 1154 1507 1651 2163 Size: 1154 353 144 512 Fragments arranged by size: 1154 512 353 144 MspA1I CmG'CkG Cuts at: 0 59 80 922 1686 2016 2106 2163 Size: 59 21 842 764 330 90 57 Fragments arranged by size: 842 764 330 90 59 57 21 NarI GG'CG_CC Cuts at: 0 19 2163 Size: 19 2144 NcoI C'CATG_G Cuts at: 0 1053 1985 2163 Size: 1053 932 178 Fragments arranged by size: 1053 932 178 NspI r_CATG'y Cuts at: 0 867 915 2163 Size: 867 48 1248 Fragments arranged by size: 1248 867 48 PasI CC'CwG_GG Cuts at: 0 654 2163 Size: 654 1509 PciI A'CATG_T Cuts at: 0 911 2163 Size: 911 1252 PfoI T'CCnGG_A Cuts at: 0 1724 1799 2163 Size: 1724 75 364 Fragments arranged by size: 1724 364 75 PmlI CAC'GTG Cuts at: 0 1794 2163 Size: 1794 369 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 701 733 2163 Size: 701 32 1430 Fragments arranged by size: 1430 701 32 PpuMI rG'GwC_Cy Cuts at: 0 195 2163 Size: 195 1968 PspOMI G'GGCC_C Cuts at: 0 1311 2163 Size: 1311 852 PstI C_TGCA'G Cuts at: 0 1500 1532 2111 2163 Size: 1500 32 579 52 Fragments arranged by size: 1500 579 52 32 PvuI CG_AT'CG Cuts at: 0 159 2163 Size: 159 2004 PvuII CAG'CTG Cuts at: 0 922 2016 2106 2163 Size: 922 1094 90 57 Fragments arranged by size: 1094 922 90 57 SacI G_AGCT'C Cuts at: 0 380 2163 Size: 380 1783 SfcI C'TryA_G Cuts at: 0 11 1496 1528 1681 2107 2163 Size: 11 1485 32 153 426 56 Fragments arranged by size: 1485 426 153 56 32 11 SfoI GGC'GCC Cuts at: 0 20 2163 Size: 20 2143 SmlI C'TyrA_G Cuts at: 0 493 803 1549 1741 1839 2163 Size: 493 310 746 192 98 324 Fragments arranged by size: 746 493 324 310 192 98 StuI AGG'CCT Cuts at: 0 1879 2163 Size: 1879 284 StyI C'CwwG_G Cuts at: 0 179 419 846 1053 1985 1991 2163 Size: 179 240 427 207 932 6 172 Fragments arranged by size: 932 427 240 207 179 172 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 590 1009 2163 Size: 590 419 1154 Fragments arranged by size: 1154 590 419 TatI w'GTAC_w Cuts at: 0 261 914 2163 Size: 261 653 1249 Fragments arranged by size: 1249 653 261 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1271 1303 2163 Size: 1271 32 860 Fragments arranged by size: 1271 860 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1998 2163 Size: 1998 165 XhoI C'TCGA_G Cuts at: 0 803 2163 Size: 803 1360 XmnI GAAnn'nnTTC Cuts at: 0 2039 2163 Size: 2039 124 ZraI GAC'GTC Cuts at: 0 936 2163 Size: 936 1227 Enzymes that do cut: AatII AccI AcuI AflIII AhdI AleI AloI AlwNI ApaI ApoI AseI AvaI AvrII BanI BanII BbeI BbsI BbvCI BcgI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrGI BssHII BstAPI BstBI BstXI BstYI Bsu36I BtgI ClaI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NspI PasI PciI PfoI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII SacI SfcI SfoI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AlfI ApaLI AscI AsiSI BaeI BamHI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BssSI BstEII BstZ17I BtgZI BtsI CspCI DraI DraIII EagI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI PshAI PsiI PspXI PsrI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmaI