GALK2 ORF clone pFN21AE3605

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3605

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Clone info.

Species

human

Clone Name

pFN21AE3605 Sequence (with vector) Restriction Map

Product ID

FHC11046

ORF Sequence

DNA sequence (1374 bp)
Protein sequence (458 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3605

Symbol

GALK2

Alias : GK2

Description

galactokinase 2, transcript variant 1

Original Clone ID

oc93605

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1374 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 458 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS42034.1 2.1e-204 99.8 GALK2 CCDS32236.1 8.1e-197 99.3 GALK2 CCDS73724.1 5.7e-194 99.8 GALK2 CCDS11728.1 2.8e-20 31.0 GALK1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR006206 40 64 PR00959 Mevalonate/galactokinase IPR000705 42 60 PR00473 Galactokinase IPR000705 107 118 PR00473 Galactokinase IPR000705 129 147 PR00473 Galactokinase IPR006206 136 158 PR00959 Mevalonate/galactokinase IPR006206 178 197 PR00959 Mevalonate/galactokinase IPR000705 330 344 PR00473 Galactokinase IPR006206 396 413 PR00959 Mevalonate/galactokinase HMMPfam IPR019539 24 73 PF10509 Galactokinase galactose-binding domain IPR006204 133 196 PF00288 GHMP kinase IPR013750 355 419 PF08544 GHMP kinase HMMTigr IPR000705 21 433 TIGR00131 Galactokinase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002035 458 137028 99.8 100.0 XP_005254336 462 137028 99.8 98.3 XP_006720524 458 137028 99.8 98.0 NP_001001556 447 137028 99.3 96.7 NP_001275960 434 137028 99.8 94.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3605 Download

>KIEE3605  1374 bp
ATGGCTACAGAGAGCCCTGCTACGCGTCGGGTCCAGGTGGCAGAACATCCTAGGTTACTG
AAGCTAAAGGAGATGTTTAACTCCAAGTTTGGATCTATTCCCAAGTTTTATGTTCGAGCA
CCAGGAAGAGTCAACATAATAGGAGAGCATATAGATTATTGTGGATATTCTGTTCTTCCT
ATGGCTGTAGAACAAGATGTGCTAATAGCTGTAGAACCTGTGAAAACGTACGCTCTCCAA
CTGGCCAATACAAATCCCTTGTATCCGGACTTCAGTACTAGTGCTAATAACATCCAGATT
GATAAAACCAAGCCTTTGTGGCACAACTATTTCTTATGTGGACTTAAAGGAATTCAGGAA
CACTTTGGTCTTAGTAACCTGACTGGAATGAACTGCCTGGTAGATGGAAATATCCCACCA
AGTTCTGGCCTCTCCAGCTCCAGTGCTTTGGTCTGTTGTGCTGGCTTGGTGACGCTCACA
GTGCTGGGAAGGAATCTATCCAAGGTGGAACTTGCAGAAATCTGTGCCAAGAGTGAGCGT
TACATTGGCACTGAAGGAGGAGGCATGGACCAGTCTATATCATTTCTTGCAGAAGAAGGA
ACTGCCAAGTTGATAGAATTTAGTCCTCTGAGGGCAACCGATGTAAAACTCCCAAGTGGA
GCAGTGTTTGTGATTGCCAACAGTTGTGTGGAGATGAATAAGGCAGCAACTTCCCATTTC
AATATCAGGGTGATGGAGTGTCGGCTGGCTGCGAAGCTCCTGGCTAAATACAAAAGCTTG
CAATGGGACAAAGTACTGAGGCTGGAGGAGGTGCAGGCTAAACTAGGGATTAGTCTAGAA
GAAATGCTGTTGGTCACAGAAGATGCCCTTCATCCTGAACCCTATAACCCTGAGGAGATC
TGCAGGTGTCTGGGAATTAGCCTGGAGGAACTCCGAACCCAAATCCTGAGTCCAAACACT
CAAGATGTGCTCATCTTCAAACTCTATCAGTGGGCAAAGCATGTGTACAGCGAGGCTGCG
CGAGTGCTCCAGTTTAAGAAGATATGTGAAGAAGCACCTGAAAACATGGTCCAGCTGCTG
GGAGAGTTGATGAACCAGAGCCACATGAGCTGCCGGGACATGTATGAGTGCAGCTGCCCC
GAGCTGGATCAGCTGGTGGACATCTGTCGGAAGTTTGGGGCTCAAGGGTCACGACTTACT
GGAGCAGGATGGGGAGGCTGTACAGTATCAATGGTACCTGCGGACAAGCTGCCCAGCTTT
CTAGCAAATGTGCACAAAGCTTATTACCAGAGGAGTGATGGAAGCTTAGCACCGGAGAAG
CAAAGTTTGTTTGCTACCAAACCTGGAGGTGGGGCTTTGGTTTTGCTTGAGGCC

ORF Protein

Cloned ORF protein sequence for pFN21AE3605 Download

>KIEE3605  458 aa
MATESPATRRVQVAEHPRLLKLKEMFNSKFGSIPKFYVRAPGRVNIIGEHIDYCGYSVLP
MAVEQDVLIAVEPVKTYALQLANTNPLYPDFSTSANNIQIDKTKPLWHNYFLCGLKGIQE
HFGLSNLTGMNCLVDGNIPPSSGLSSSSALVCCAGLVTLTVLGRNLSKVELAEICAKSER
YIGTEGGGMDQSISFLAEEGTAKLIEFSPLRATDVKLPSGAVFVIANSCVEMNKAATSHF
NIRVMECRLAAKLLAKYKSLQWDKVLRLEEVQAKLGISLEEMLLVTEDALHPEPYNPEEI
CRCLGISLEELRTQILSPNTQDVLIFKLYQWAKHVYSEAARVLQFKKICEEAPENMVQLL
GELMNQSHMSCRDMYECSCPELDQLVDICRKFGAQGSRLTGAGWGGCTVSMVPADKLPSF
LANVHKAYYQRSDGSLAPEKQSLFATKPGGGALVLLEA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3605 Download

>pFN21AE3605  6079 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTACAGAGAGCCCTGCTACGCGTCGGGTCCA
GGTGGCAGAACATCCTAGGTTACTGAAGCTAAAGGAGATGTTTAACTCCAAGTTTGGATC
TATTCCCAAGTTTTATGTTCGAGCACCAGGAAGAGTCAACATAATAGGAGAGCATATAGA
TTATTGTGGATATTCTGTTCTTCCTATGGCTGTAGAACAAGATGTGCTAATAGCTGTAGA
ACCTGTGAAAACGTACGCTCTCCAACTGGCCAATACAAATCCCTTGTATCCGGACTTCAG
TACTAGTGCTAATAACATCCAGATTGATAAAACCAAGCCTTTGTGGCACAACTATTTCTT
ATGTGGACTTAAAGGAATTCAGGAACACTTTGGTCTTAGTAACCTGACTGGAATGAACTG
CCTGGTAGATGGAAATATCCCACCAAGTTCTGGCCTCTCCAGCTCCAGTGCTTTGGTCTG
TTGTGCTGGCTTGGTGACGCTCACAGTGCTGGGAAGGAATCTATCCAAGGTGGAACTTGC
AGAAATCTGTGCCAAGAGTGAGCGTTACATTGGCACTGAAGGAGGAGGCATGGACCAGTC
TATATCATTTCTTGCAGAAGAAGGAACTGCCAAGTTGATAGAATTTAGTCCTCTGAGGGC
AACCGATGTAAAACTCCCAAGTGGAGCAGTGTTTGTGATTGCCAACAGTTGTGTGGAGAT
GAATAAGGCAGCAACTTCCCATTTCAATATCAGGGTGATGGAGTGTCGGCTGGCTGCGAA
GCTCCTGGCTAAATACAAAAGCTTGCAATGGGACAAAGTACTGAGGCTGGAGGAGGTGCA
GGCTAAACTAGGGATTAGTCTAGAAGAAATGCTGTTGGTCACAGAAGATGCCCTTCATCC
TGAACCCTATAACCCTGAGGAGATCTGCAGGTGTCTGGGAATTAGCCTGGAGGAACTCCG
AACCCAAATCCTGAGTCCAAACACTCAAGATGTGCTCATCTTCAAACTCTATCAGTGGGC
AAAGCATGTGTACAGCGAGGCTGCGCGAGTGCTCCAGTTTAAGAAGATATGTGAAGAAGC
ACCTGAAAACATGGTCCAGCTGCTGGGAGAGTTGATGAACCAGAGCCACATGAGCTGCCG
GGACATGTATGAGTGCAGCTGCCCCGAGCTGGATCAGCTGGTGGACATCTGTCGGAAGTT
TGGGGCTCAAGGGTCACGACTTACTGGAGCAGGATGGGGAGGCTGTACAGTATCAATGGT
ACCTGCGGACAAGCTGCCCAGCTTTCTAGCAAATGTGCACAAAGCTTATTACCAGAGGAG
TGATGGAAGCTTAGCACCGGAGAAGCAAAGTTTGTTTGCTACCAAACCTGGAGGTGGGGC
TTTGGTTTTGCTTGAGGCCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT