################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII AccI AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AviII BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BpuAI BsaAI BsaBI BsaHI BsaI BsiEI BsmAI BsmBI BsmI BspDI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII Hin1I HpaI KasI KspI MamI MfeI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspI StuI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 1233 GGTACC G^GTACC AccIII 264 TCCGGA T^CCGGA AciI 1240 GCGG CCGC(-3/-1) Alw44I 1270 GTGCAC G^TGCAC AlwNI 1072 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 889 CCTGAGG CC^T[ACGT]AGG ApaLI 1270 GTGCAC G^TGCAC Asp718I 1233 GGTACC G^GTACC AvaI 1138 CCCGAG C^[CT]CG[GA]G AvrII 49 CCTAGG C^CTAGG BalI 242 TGGCCA TGG^CCA BanI 1233 GGTACC G^G[CT][GA]CC BciVI 261 GTATCC GTATCC(6/5) BcnI 1113 CCGGG CC^[GC]GG BglI 312 GCCTTTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 896 AGATCT A^GATCT BlnI 49 CCTAGG C^CTAGG BlpI 1304 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1304 GCTTAGC GC^T[ACGT]AGC BseAI 264 TCCGGA T^CCGGA BsiWI 227 CGTACG C^GTACG BsoBI 1138 CCCGAG C^[CT]CG[GA]G BspEI 264 TCCGGA T^CCGGA BspLU11I 1118 ACATGT A^CATGT BsrDI 780 GCAATG GCAATG(2/0) BstXI 440 CCAGTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 889 CCTGAGG CC^T[ACGT]AGG CelII 1304 GCTTAGC GC^T[ACGT]AGC CfoI 1018 GCGC GCG^C EaeI 242 TGGCCA [CT]^GGCC[GA] EarI 125 GAAGAG CTCTTC(1/4) Eco81I 889 CCTGAGG CC^T[ACGT]AGG EcoRI 350 GAATTC G^AATTC HhaI 1018 GCGC GCG^C HinP1I 1018 GCGC G^CGC HincII 130 GTCAAC GT[CT]^[GA]AC HindII 130 GTCAAC GT[CT]^[GA]AC KpnI 1233 GGTACC GGTAC^C Ksp632I 125 GAAGAG CTCTTC(1/4) MaeII 226 ACGT A^CGT MluI 22 ACGCGT A^CGCGT MluNI 242 TGGCCA TGG^CCA MroI 264 TCCGGA T^CCGGA MscI 242 TGGCCA TGG^CCA NciI 1113 CCGGG CC^[GC]GG PshAI 1159 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 900 CTGCAG CTGCA^G SfaNI 862 GATGC GCATC(5/9) SpeI 277 ACTAGT A^CTAGT TaqI 114 TCGA T^CGA TfiI 492 GAATC G^A[AT]TC TthHB8I 114 TCGA T^CGA XbaI 834 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 23 CGCG CG^CG 1019 CGCG AcsI 350 GAATTC [GA]^AATT[CT] 616 GAATTT AflIII 22 ACGCGT A^C[GA][CT]GT 1118 ACATGT AlwI 91 GGATC GGATC(4/5) 1146 GGATC ApoI 350 GAATTC [GA]^AATT[CT] 616 GAATTT BanII 12 GAGCCC G[GA]GC[CT]^C 1178 GGGCTC BsaJI 49 CCTAGG C^C[ACGT][ACGT]GG 500 CCAAGG BsaWI 264 TCCGGA [AT]^CCGG[AT] 1311 ACCGGA BsgI 811 GTGCAG GTGCAG(16/14) 1128 GTGCAG BsmFI 785 GGGAC GGGAC(10/14) 1115 GGGAC Bsp1407I 1004 TGTACA T^GTACA 1219 TGTACA BspMI 1236 ACCTGC ACCTGC(4/8) 902 GCAGGT BsrGI 1004 TGTACA T^GTACA 1219 TGTACA BstUI 23 CGCG CG^CG 1019 CGCG BstYI 91 GGATCT [GA]^GATC[CT] 896 AGATCT EcoT14I 49 CCTAGG C^C[AT][AT]GG 500 CCAAGG HgaI 471 GACGC GACGC(5/10) 24 GCGTC HphI 468 GGTGA GGTGA(8/7) 729 GGTGA MflI 91 GGATCT [GA]^GATC[CT] 896 AGATCT MvnI 23 CGCG CG^CG 1019 CGCG NlaIV 29 GGGTCC GG[ACGT]^[ACGT]CC 1233 GGTACC NspI 999 GCATGT [GA]CATG^[CT] 1118 ACATGT PflMI 418 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1071 CCAGCTGCTGG PleI 128 GAGTC GAGTC(4/5) 948 GAGTC ScaI 274 AGTACT AGT^ACT 792 AGTACT SspBI 1004 TGTACA T^GTACA 1219 TGTACA StyI 49 CCTAGG C^C[AT][AT]GG 500 CCAAGG Van91I 418 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1071 CCAGCTGCTGG XcmI 677 CCAACAGTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1287 CCAGAGGAGTGATGG XhoII 91 GGATCT [GA]^GATC[CT] 896 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AfaI AluI AspHI AvaII BbvI BfaI BmyI BpmI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII ItaI MaeI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII PvuII RsaI Sau3AI Sau96I ScrFI SfcI SmlI Tru9I TseI Tsp45I Tsp509I TspRI