PPL ORF clone pFN21AE2387

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE2387

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Clone info.

Species

human

Clone Name

pFN21AE2387 Sequence (with vector) Restriction Map

Product ID

FHC12300

Flexi Clone PID

FXC12300

ORF Sequence

DNA sequence (5268 bp)
Protein sequence (1756 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2387

Symbol

PPL

Description

periplakin

Original Clone ID

cp01546

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 5268 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 1756 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10526.1 0 99.8 PPL CCDS11737.1 8.4e-46 30.9 EVPL CCDS82207.1 3.9e-38 30.5 EVPL CCDS4959.1 1.3e-16 23.4 DST CCDS4501.1 2.1e-16 22.7 DSP The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001101 1660 1692 PF00681 Plectin repeat HMMSmart IPR018159 126 213 SM00150 Spectrin/alpha-actinin IPR018159 216 317 SM00150 Spectrin/alpha-actinin IPR018159 323 485 SM00150 Spectrin/alpha-actinin IPR018159 505 612 SM00150 Spectrin/alpha-actinin IPR018159 733 861 SM00150 Spectrin/alpha-actinin IPR001101 1651 1685 SM00250 Plectin repeat IPR001101 1700 1735 SM00250 Plectin repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002696 1756 602871 99.9 100.0 XP_006720965 1769 602871 99.8 98.9 XP_016878864 1772 602871 98.9 100.0 XP_016878863 1785 602871 98.9 98.9 NP_001979 2033 601590 30.9 99.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2387 Download

>KIEE2387  5268 bp
ATGAACTCGCTCTTCAGGAAGAGAAACAAAGGCAAATACAGCCCCACTGTGCAGACCCGG
AGCATCTCTAACAAGGAGCTCTCGGAGCTGATCGAGCAGCTGCAGAAGAATGCCGACCAG
GTGGAGAAGAACATCGTGGACACAGAGGCCAAGATGCAGAGTGACCTGGCTCGGCTGCAG
GAGGGTCGGCAGCCTGAGCACCGGGACGTGACCCTGCAGAAGGTGTTGGACTCTGAGAAG
CTGCTCTATGTGCTAGAGGCGGATGCGGCCATTGCCAAGCACATGAAGCACCCACAGGGG
GACATGATCGCCGAGGATATCCGCCAGCTGAAGGAGCGTGTGACCAACCTGCGCGGGAAA
CACAAGCAGATCTACAGGCTGGCGGTGAAGGAAGTGGATCCACAGGTCAACTGGGCGGCA
CTGGTGGAGGAGAAGCTGGACAAGCTGAACAACCAGAGCTTTGGGACTGACCTGCCGCTG
GTGGACCACCAAGTGGAGGAGCATAACATCTTCCACAATGAGGTCAAGGCCATCGGGCCC
CACCTGGCCAAGGACGGGGACAAGGAGCAGAACAGCGAACTCCGGGCCAAGTACCAGAAA
CTGCTGGCAGCATCACAGGCCCGGCAGCAGCACCTGAGTTCGCTGCAGGACTACATGCAG
CGCTGCACCAATGAGCTGTACTGGCTGGACCAGCAGGCCAAGGGCCGCATGCAGTACGAC
TGGAGTGACCGCAACCTCGACTACCCCAGCCGCCGGCGCCAGTATGAGAATTTCATCAAC
CGGAACCTGGAGGCCAAAGAGGAGAGAATCAACAAACTGCACAGCGAGGGCGACCAGCTG
CTGGCGGCCGAGCACCCCGGGAGGAACTCCATTGAGGCGCACATGGAGGCTGTGCACGCA
GACTGGAAGGAGTACCTGAACCTGCTCATCTGCGAGGAGAGCCACCTCAAGTACATGGAG
GACTACCACCAGTTTCACGAAGACGTGAAGGACGCTCAGGAGCTGCTGCGCAAGGTGGAC
TCGGACCTGAACCAGAAGTATGGCCCTGACTTCAAGGACCGGTACCAGATTGAGCTGCTG
CTGCGGGAGCTGGATGACCAGGAGAAGGTGCTGGACAAGTATGAGGACGTGGTGCAGGGG
CTGCAGAAGCGAGGCCAGCAGGTGGTGCCCCTCAAGTACCGCCGGGAGACTCCGCTCAAG
CCCATCCCCGTGGAGGCACTCTGTGACTTTGAGGGGGAGCAGGGCCTGATCTCGCGGGGC
TACAGCTACACCCTGCAGAAGAACAACGGGGAGAGCTGGGAGCTCATGGACAGCGCTGGG
AACAAGCTGATTGCTCCGGCCGTCTGTTTTGTGATCCCCCCCACAGACCCTGAGGCCCTG
GCTCTGGCTGACAGCCTGGGCAGCCAGTACCGGAGCGTGCGGCAGAAGGCAGCTGGGAGC
AAACGCACGCTGCAGCAGCGGTATGAGGTGCTGAAGACCGAGAATCCCGGAGATGCCTCT
GACCTACAGGGGCGGCAGCTGCTGGCTGGCTTGGACAAGGTGGCCAGCGACCTGGACCGG
CAGGAGAAGGCCATCACAGGGATCCTGCGGCCACCACTGGAGCAAGGCCGGGCTGTGCAG
GACAGTGCCGAGCGGGCCAAGGACCTCAAGAACATCACCAACGAGCTACTGCGGATTGAA
CCTGAGAAGACGCGGAGCACGGCTGAGGGCGAAGCCTTCATCCAGGCCCTCCCAGGCAGT
GGCACCACACCCCTGCTGAGGACCCAGGTGGAGGACACCAACCGGAAATACGAGCACCTC
CTGCAGCTGCTGGACTTGGCCCAGGAGAAGGTTGATGTGGCCAACCGCCTGGAGAAGAGC
CTGCAGCAGAGCTGGGAGTTGCTGGCCACACACGAGAACCATCTGAATCAGGATGACACA
GTGCCTGAGAGCAGCCGTGTCCTGGACAGCAAGGGGCAGGAGCTGGCGGCCATGGCCTGT
GAGTTACAGGCCCAGAAGTCCCTCCTGGGTGAGGTGGAGCAGAACTTGCAGGCGGCCAAG
CAGTGCTCGAGCACACTGGCCAGCCGCTTCCAGGAGCACTGCCCGGACCTGGAGCGCCAG
GAGGCCGAGGTGCACAAGCTGGGCCAGCGTTTCAACAACCTGCGCCAGCAGGTGGAACGC
AGGGCGCAGAGCCTACAGAGCGCCAAGGCAGCCTACGAGCACTTCCACCGCGGCCATGAC
CACGTGCTGCAGTTCCTAGTCAGCATCCCCAGTTACGAGCCCCAGGAGACAGACAGCCTC
AGCCAGATGGAGACCAAGCTGAAGAACCAGAAGAACCTGCTAGATGAGATAGCAAGTAGG
GAGCAGGAAGTACAGAAGATCTGTGCCAATTCCCAGCAGTACCAGCAAGCTGTAAAGGAC
TATGAGTTAGAAGCAGAAAAACTAAGGTCTCTTCTCGACTTGGAGAATGGAAGGAGCAGC
CACGTGAGCAAGAGAGCCAGGCTCCAATCTCCTGCCACCAAAGTGAAGGAAGAGGAAGCA
GCACTTGCCGCCAAGTTCACTGAAGTTTATGCCATCAACAGACAGAGGCTGCAGAATCTG
GAGTTTGCTCTGAATCTCCTCAGACAGCAGCCGGAAGTAGAAGTGACCCATGAGACCCTG
CAAAGGAATAGGCCGGACTCTGGAGTGGAGGAGGCGTGGAAGATCAGGAAGGAACTGGAT
GAGGAGACTGAGCGGAGGCGGCAGCTGGAGAACGAGGTCAAGAGCACCCAGGAAGAAATC
TGGACCTTGAGGAATCAGGGGCCTCAGGAATCGGTGGTGAGGAAGGAGGTGCTCAAGAAG
GTGCCGGATCCCGTGCTGGAGGAGAGCTTCCAGCAGCTGCAGCGGACGCTGGCAGAGGAG
CAGCACAAGAACCAGCTGCTGCAGGAGGAGCTGGAGGCACTGCAGCTGCAGCTGCGTGCC
CTGGAGCAGGAGACCAGAGACGGGGGGCAGGAGTACGTGGTCAAGGAGGTCCTGCGCATC
GAGCCTGACAGGGCCCAGGCGGATGAGGTCTTGCAGCTGCGGGAGGAGCTGGAGGCACTG
AGGCGGCAGAAGGGCGCCCGGGAGGCAGAGGTGCTCCTCCTGCAGCAGCGTGTGGCCGCC
CTGGCTGAAGAGAAGAGCCGGGCGCAGGAGAAGGTCACAGAGAAAGAGGTGGTGAAACTG
CAGAATGACCCCCAGCTGGAGGCAGAGTACCAGCAGCTGCAGGAGGACCACCAGCGCCAG
GACCAGCTCAGGGAGAAGCAGGAGGAGGAGCTGAGCTTCCTCCAGGACAAGCTCAAGAGG
CTAGAGAAGGAGCGGGCCATGGCCGAGGGCAAGATCACCGTCAAGGAGGTGCTCAAGGTG
GAGAAGGACGCGGCCACCGAGAGGGAGGTCAGCGATCTCACCCGCCAATATGAGGACGAG
GCTGCCAAGGCTCGCGCTAGCCAGAGGGAGAAGACGGAGCTGCTCCGAAAGATATGGGCC
TTGGAGGAGGAGAACGCCAAAGTGGTGGTGCAGGAGAAGGTGCGGGAGATCGTGCGGCCA
GACCCCAAGGCGGAAAGTGAAGTGGCGAACCTCCGCCTGGAGCTTGTGGAGCAGGAGCGA
AAGTACCGGGGTGCCGAGGAGCAGCTCCGGAGCTACCAGAGTGAGCTGGAGGCCCTCAGG
AGGCGAGGCCCCCAGGTGGAAGTCAAAGAGGTGACTAAGGAAGTCATTAAGTACAAGACT
GACCCTGAGATGGAGAAGGAGCTTCAGCGGCTCAGGGAGGAGATCGTGGACAAGACCAGA
CTGATCGAAAGGTGTGATTTAGAGATCTACCAGCTGAAAAAGGAAATCCAGGCCCTGAAA
GACACCAAACCCCAGGTCCAGACCAAAGAGGTGGTCCAGGAGATCCTCCAATTCCAAGAA
GACCCTCAAACCAAGGAGGAGGTGGCGTCTCTGAGGGCAAAGCTCTCAGAGGAGCAGAAG
AAACAAGTGGATCTGGAGAGGGAAAGAGCTTCCCAGGAAGAGCAGATCGCCCGGAAAGAG
GAGGAGCTCTCGCGGGTGAAGGAAAGGGTGGTGCAGCAGGAGGTGGTCAGGTATGAGGAG
GAGCCAGGCCTCCGGGCCGAGGCGAGTGCCTTTGCCGAGAGCATCGATGTGGAGCTGCGG
CAGATTGACAAGCTGCGGGCAGAGCTGCGGCGGCTGCAGCGCCGGCGCACCGAGCTTGAG
CGGCAGCTGGAGGAGCTAGAGCGCGAGCGGCAGGCCCGCAGGGAGGCCGAGCGCGAGGTA
CAGCGGTTGCAGCAGCGGCTGGCAGCGCTGGAGCAGGAAGAAGCTGAGGCCCGTGAGAAG
GTAACCCATACGCAGAAGGTGGTGCTGCAGCAGGACCCGCAGCAGGCGCGAGAGCATGCC
CTGCTCCGACTCCAGCTGGAAGAAGAGCAGCACCGGCGGCAGCTCCTGGAGGGGGAGCTC
GAGACCCTCCGGAGGAAACTGGCTGCACTGGAGAAGGCGGAGGTCAAGGAGAAGGTGGTG
CTCTCCGAGAGTGTCCAGGTGGAGAAGGGCGACACCGAGCAAGAGATCCAGAGGCTCAAG
AGCAGCCTGGAGGAGGAGAGCCGCAGCAAGCGCGAGCTGGACGTCGAGGTGAGCCGGCTG
GAAGCCAGGCTTTCGGAGCTGGAATTCCATAACTCCAAGTCATCCAAGGAACTAGACTTT
CTGAGGGAAGAGAACCACAAATTACAGCTGGAGAGGGAAAACCTGCAGCTGGAGACCCGA
AGGCTCCAATCGGAAATCAACATGGCAGCGACGGAAACACGAGACCTGCGGAACATGACC
GTGGCGGACTCTGGGACCAACCATGACTCCAGACTGTGGTCCCTGGAGAGGGAACTGGAT
GACCTCAAGAGGCTCTCCAAGGACAAAGACCTCGAGATCGACGAGCTGCAGAAGCGCCTG
GGCTCCGTGGCCGTCAAGCGGGAGCAGCGGGAGAACCACCTGCGGCGCTCCATCGTAGTC
ATCCACCCTGACACAGGCCGCGAGCTGTCCCCGGAGGAAGCCCACCGTGCCGGGCTCATT
GACTGGAACATGTTCGTGAAACTCAGAAGCCAGGAGTGCGACTGGGAGGAGATCTCAGTG
AAGGGTCCCAATGGGGAGTCCTCAGTGATACACGACAGGAAGTCTGGCAAGAAGTTCTCC
ATCGAAGAGGCCCTGCAGAGTGGCAGGCTGACCCCTGCTCAGTATGACCGCTATGTCAAC
AAGGATATGTCCATCCAGGAGCTGGCGGTCTTGGTATCTGGGCAGAAG

ORF Protein

Cloned ORF protein sequence for pFN21AE2387 Download

>KIEE2387  1756 aa
MNSLFRKRNKGKYSPTVQTRSISNKELSELIEQLQKNADQVEKNIVDTEAKMQSDLARLQ
EGRQPEHRDVTLQKVLDSEKLLYVLEADAAIAKHMKHPQGDMIAEDIRQLKERVTNLRGK
HKQIYRLAVKEVDPQVNWAALVEEKLDKLNNQSFGTDLPLVDHQVEEHNIFHNEVKAIGP
HLAKDGDKEQNSELRAKYQKLLAASQARQQHLSSLQDYMQRCTNELYWLDQQAKGRMQYD
WSDRNLDYPSRRRQYENFINRNLEAKEERINKLHSEGDQLLAAEHPGRNSIEAHMEAVHA
DWKEYLNLLICEESHLKYMEDYHQFHEDVKDAQELLRKVDSDLNQKYGPDFKDRYQIELL
LRELDDQEKVLDKYEDVVQGLQKRGQQVVPLKYRRETPLKPIPVEALCDFEGEQGLISRG
YSYTLQKNNGESWELMDSAGNKLIAPAVCFVIPPTDPEALALADSLGSQYRSVRQKAAGS
KRTLQQRYEVLKTENPGDASDLQGRQLLAGLDKVASDLDRQEKAITGILRPPLEQGRAVQ
DSAERAKDLKNITNELLRIEPEKTRSTAEGEAFIQALPGSGTTPLLRTQVEDTNRKYEHL
LQLLDLAQEKVDVANRLEKSLQQSWELLATHENHLNQDDTVPESSRVLDSKGQELAAMAC
ELQAQKSLLGEVEQNLQAAKQCSSTLASRFQEHCPDLERQEAEVHKLGQRFNNLRQQVER
RAQSLQSAKAAYEHFHRGHDHVLQFLVSIPSYEPQETDSLSQMETKLKNQKNLLDEIASR
EQEVQKICANSQQYQQAVKDYELEAEKLRSLLDLENGRSSHVSKRARLQSPATKVKEEEA
ALAAKFTEVYAINRQRLQNLEFALNLLRQQPEVEVTHETLQRNRPDSGVEEAWKIRKELD
EETERRRQLENEVKSTQEEIWTLRNQGPQESVVRKEVLKKVPDPVLEESFQQLQRTLAEE
QHKNQLLQEELEALQLQLRALEQETRDGGQEYVVKEVLRIEPDRAQADEVLQLREELEAL
RRQKGAREAEVLLLQQRVAALAEEKSRAQEKVTEKEVVKLQNDPQLEAEYQQLQEDHQRQ
DQLREKQEEELSFLQDKLKRLEKERAMAEGKITVKEVLKVEKDAATEREVSDLTRQYEDE
AAKARASQREKTELLRKIWALEEENAKVVVQEKVREIVRPDPKAESEVANLRLELVEQER
KYRGAEEQLRSYQSELEALRRRGPQVEVKEVTKEVIKYKTDPEMEKELQRLREEIVDKTR
LIERCDLEIYQLKKEIQALKDTKPQVQTKEVVQEILQFQEDPQTKEEVASLRAKLSEEQK
KQVDLERERASQEEQIARKEEELSRVKERVVQQEVVRYEEEPGLRAEASAFAESIDVELR
QIDKLRAELRRLQRRRTELERQLEELERERQARREAEREVQRLQQRLAALEQEEAEAREK
VTHTQKVVLQQDPQQAREHALLRLQLEEEQHRRQLLEGELETLRRKLAALEKAEVKEKVV
LSESVQVEKGDTEQEIQRLKSSLEEESRSKRELDVEVSRLEARLSELEFHNSKSSKELDF
LREENHKLQLERENLQLETRRLQSEINMAATETRDLRNMTVADSGTNHDSRLWSLERELD
DLKRLSKDKDLEIDELQKRLGSVAVKREQRENHLRRSIVVIHPDTGRELSPEEAHRAGLI
DWNMFVKLRSQECDWEEISVKGPNGESSVIHDRKSGKKFSIEEALQSGRLTPAQYDRYVN
KDMSIQELAVLVSGQK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2387 Download

>pFN21AE2387  9973 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAACTCGCTCTTCAGGAAGAGAAACAAAGGCAA
ATACAGCCCCACTGTGCAGACCCGGAGCATCTCTAACAAGGAGCTCTCGGAGCTGATCGA
GCAGCTGCAGAAGAATGCCGACCAGGTGGAGAAGAACATCGTGGACACAGAGGCCAAGAT
GCAGAGTGACCTGGCTCGGCTGCAGGAGGGTCGGCAGCCTGAGCACCGGGACGTGACCCT
GCAGAAGGTGTTGGACTCTGAGAAGCTGCTCTATGTGCTAGAGGCGGATGCGGCCATTGC
CAAGCACATGAAGCACCCACAGGGGGACATGATCGCCGAGGATATCCGCCAGCTGAAGGA
GCGTGTGACCAACCTGCGCGGGAAACACAAGCAGATCTACAGGCTGGCGGTGAAGGAAGT
GGATCCACAGGTCAACTGGGCGGCACTGGTGGAGGAGAAGCTGGACAAGCTGAACAACCA
GAGCTTTGGGACTGACCTGCCGCTGGTGGACCACCAAGTGGAGGAGCATAACATCTTCCA
CAATGAGGTCAAGGCCATCGGGCCCCACCTGGCCAAGGACGGGGACAAGGAGCAGAACAG
CGAACTCCGGGCCAAGTACCAGAAACTGCTGGCAGCATCACAGGCCCGGCAGCAGCACCT
GAGTTCGCTGCAGGACTACATGCAGCGCTGCACCAATGAGCTGTACTGGCTGGACCAGCA
GGCCAAGGGCCGCATGCAGTACGACTGGAGTGACCGCAACCTCGACTACCCCAGCCGCCG
GCGCCAGTATGAGAATTTCATCAACCGGAACCTGGAGGCCAAAGAGGAGAGAATCAACAA
ACTGCACAGCGAGGGCGACCAGCTGCTGGCGGCCGAGCACCCCGGGAGGAACTCCATTGA
GGCGCACATGGAGGCTGTGCACGCAGACTGGAAGGAGTACCTGAACCTGCTCATCTGCGA
GGAGAGCCACCTCAAGTACATGGAGGACTACCACCAGTTTCACGAAGACGTGAAGGACGC
TCAGGAGCTGCTGCGCAAGGTGGACTCGGACCTGAACCAGAAGTATGGCCCTGACTTCAA
GGACCGGTACCAGATTGAGCTGCTGCTGCGGGAGCTGGATGACCAGGAGAAGGTGCTGGA
CAAGTATGAGGACGTGGTGCAGGGGCTGCAGAAGCGAGGCCAGCAGGTGGTGCCCCTCAA
GTACCGCCGGGAGACTCCGCTCAAGCCCATCCCCGTGGAGGCACTCTGTGACTTTGAGGG
GGAGCAGGGCCTGATCTCGCGGGGCTACAGCTACACCCTGCAGAAGAACAACGGGGAGAG
CTGGGAGCTCATGGACAGCGCTGGGAACAAGCTGATTGCTCCGGCCGTCTGTTTTGTGAT
CCCCCCCACAGACCCTGAGGCCCTGGCTCTGGCTGACAGCCTGGGCAGCCAGTACCGGAG
CGTGCGGCAGAAGGCAGCTGGGAGCAAACGCACGCTGCAGCAGCGGTATGAGGTGCTGAA
GACCGAGAATCCCGGAGATGCCTCTGACCTACAGGGGCGGCAGCTGCTGGCTGGCTTGGA
CAAGGTGGCCAGCGACCTGGACCGGCAGGAGAAGGCCATCACAGGGATCCTGCGGCCACC
ACTGGAGCAAGGCCGGGCTGTGCAGGACAGTGCCGAGCGGGCCAAGGACCTCAAGAACAT
CACCAACGAGCTACTGCGGATTGAACCTGAGAAGACGCGGAGCACGGCTGAGGGCGAAGC
CTTCATCCAGGCCCTCCCAGGCAGTGGCACCACACCCCTGCTGAGGACCCAGGTGGAGGA
CACCAACCGGAAATACGAGCACCTCCTGCAGCTGCTGGACTTGGCCCAGGAGAAGGTTGA
TGTGGCCAACCGCCTGGAGAAGAGCCTGCAGCAGAGCTGGGAGTTGCTGGCCACACACGA
GAACCATCTGAATCAGGATGACACAGTGCCTGAGAGCAGCCGTGTCCTGGACAGCAAGGG
GCAGGAGCTGGCGGCCATGGCCTGTGAGTTACAGGCCCAGAAGTCCCTCCTGGGTGAGGT
GGAGCAGAACTTGCAGGCGGCCAAGCAGTGCTCGAGCACACTGGCCAGCCGCTTCCAGGA
GCACTGCCCGGACCTGGAGCGCCAGGAGGCCGAGGTGCACAAGCTGGGCCAGCGTTTCAA
CAACCTGCGCCAGCAGGTGGAACGCAGGGCGCAGAGCCTACAGAGCGCCAAGGCAGCCTA
CGAGCACTTCCACCGCGGCCATGACCACGTGCTGCAGTTCCTAGTCAGCATCCCCAGTTA
CGAGCCCCAGGAGACAGACAGCCTCAGCCAGATGGAGACCAAGCTGAAGAACCAGAAGAA
CCTGCTAGATGAGATAGCAAGTAGGGAGCAGGAAGTACAGAAGATCTGTGCCAATTCCCA
GCAGTACCAGCAAGCTGTAAAGGACTATGAGTTAGAAGCAGAAAAACTAAGGTCTCTTCT
CGACTTGGAGAATGGAAGGAGCAGCCACGTGAGCAAGAGAGCCAGGCTCCAATCTCCTGC
CACCAAAGTGAAGGAAGAGGAAGCAGCACTTGCCGCCAAGTTCACTGAAGTTTATGCCAT
CAACAGACAGAGGCTGCAGAATCTGGAGTTTGCTCTGAATCTCCTCAGACAGCAGCCGGA
AGTAGAAGTGACCCATGAGACCCTGCAAAGGAATAGGCCGGACTCTGGAGTGGAGGAGGC
GTGGAAGATCAGGAAGGAACTGGATGAGGAGACTGAGCGGAGGCGGCAGCTGGAGAACGA
GGTCAAGAGCACCCAGGAAGAAATCTGGACCTTGAGGAATCAGGGGCCTCAGGAATCGGT
GGTGAGGAAGGAGGTGCTCAAGAAGGTGCCGGATCCCGTGCTGGAGGAGAGCTTCCAGCA
GCTGCAGCGGACGCTGGCAGAGGAGCAGCACAAGAACCAGCTGCTGCAGGAGGAGCTGGA
GGCACTGCAGCTGCAGCTGCGTGCCCTGGAGCAGGAGACCAGAGACGGGGGGCAGGAGTA
CGTGGTCAAGGAGGTCCTGCGCATCGAGCCTGACAGGGCCCAGGCGGATGAGGTCTTGCA
GCTGCGGGAGGAGCTGGAGGCACTGAGGCGGCAGAAGGGCGCCCGGGAGGCAGAGGTGCT
CCTCCTGCAGCAGCGTGTGGCCGCCCTGGCTGAAGAGAAGAGCCGGGCGCAGGAGAAGGT
CACAGAGAAAGAGGTGGTGAAACTGCAGAATGACCCCCAGCTGGAGGCAGAGTACCAGCA
GCTGCAGGAGGACCACCAGCGCCAGGACCAGCTCAGGGAGAAGCAGGAGGAGGAGCTGAG
CTTCCTCCAGGACAAGCTCAAGAGGCTAGAGAAGGAGCGGGCCATGGCCGAGGGCAAGAT
CACCGTCAAGGAGGTGCTCAAGGTGGAGAAGGACGCGGCCACCGAGAGGGAGGTCAGCGA
TCTCACCCGCCAATATGAGGACGAGGCTGCCAAGGCTCGCGCTAGCCAGAGGGAGAAGAC
GGAGCTGCTCCGAAAGATATGGGCCTTGGAGGAGGAGAACGCCAAAGTGGTGGTGCAGGA
GAAGGTGCGGGAGATCGTGCGGCCAGACCCCAAGGCGGAAAGTGAAGTGGCGAACCTCCG
CCTGGAGCTTGTGGAGCAGGAGCGAAAGTACCGGGGTGCCGAGGAGCAGCTCCGGAGCTA
CCAGAGTGAGCTGGAGGCCCTCAGGAGGCGAGGCCCCCAGGTGGAAGTCAAAGAGGTGAC
TAAGGAAGTCATTAAGTACAAGACTGACCCTGAGATGGAGAAGGAGCTTCAGCGGCTCAG
GGAGGAGATCGTGGACAAGACCAGACTGATCGAAAGGTGTGATTTAGAGATCTACCAGCT
GAAAAAGGAAATCCAGGCCCTGAAAGACACCAAACCCCAGGTCCAGACCAAAGAGGTGGT
CCAGGAGATCCTCCAATTCCAAGAAGACCCTCAAACCAAGGAGGAGGTGGCGTCTCTGAG
GGCAAAGCTCTCAGAGGAGCAGAAGAAACAAGTGGATCTGGAGAGGGAAAGAGCTTCCCA
GGAAGAGCAGATCGCCCGGAAAGAGGAGGAGCTCTCGCGGGTGAAGGAAAGGGTGGTGCA
GCAGGAGGTGGTCAGGTATGAGGAGGAGCCAGGCCTCCGGGCCGAGGCGAGTGCCTTTGC
CGAGAGCATCGATGTGGAGCTGCGGCAGATTGACAAGCTGCGGGCAGAGCTGCGGCGGCT
GCAGCGCCGGCGCACCGAGCTTGAGCGGCAGCTGGAGGAGCTAGAGCGCGAGCGGCAGGC
CCGCAGGGAGGCCGAGCGCGAGGTACAGCGGTTGCAGCAGCGGCTGGCAGCGCTGGAGCA
GGAAGAAGCTGAGGCCCGTGAGAAGGTAACCCATACGCAGAAGGTGGTGCTGCAGCAGGA
CCCGCAGCAGGCGCGAGAGCATGCCCTGCTCCGACTCCAGCTGGAAGAAGAGCAGCACCG
GCGGCAGCTCCTGGAGGGGGAGCTCGAGACCCTCCGGAGGAAACTGGCTGCACTGGAGAA
GGCGGAGGTCAAGGAGAAGGTGGTGCTCTCCGAGAGTGTCCAGGTGGAGAAGGGCGACAC
CGAGCAAGAGATCCAGAGGCTCAAGAGCAGCCTGGAGGAGGAGAGCCGCAGCAAGCGCGA
GCTGGACGTCGAGGTGAGCCGGCTGGAAGCCAGGCTTTCGGAGCTGGAATTCCATAACTC
CAAGTCATCCAAGGAACTAGACTTTCTGAGGGAAGAGAACCACAAATTACAGCTGGAGAG
GGAAAACCTGCAGCTGGAGACCCGAAGGCTCCAATCGGAAATCAACATGGCAGCGACGGA
AACACGAGACCTGCGGAACATGACCGTGGCGGACTCTGGGACCAACCATGACTCCAGACT
GTGGTCCCTGGAGAGGGAACTGGATGACCTCAAGAGGCTCTCCAAGGACAAAGACCTCGA
GATCGACGAGCTGCAGAAGCGCCTGGGCTCCGTGGCCGTCAAGCGGGAGCAGCGGGAGAA
CCACCTGCGGCGCTCCATCGTAGTCATCCACCCTGACACAGGCCGCGAGCTGTCCCCGGA
GGAAGCCCACCGTGCCGGGCTCATTGACTGGAACATGTTCGTGAAACTCAGAAGCCAGGA
GTGCGACTGGGAGGAGATCTCAGTGAAGGGTCCCAATGGGGAGTCCTCAGTGATACACGA
CAGGAAGTCTGGCAAGAAGTTCTCCATCGAAGAGGCCCTGCAGAGTGGCAGGCTGACCCC
TGCTCAGTATGACCGCTATGTCAACAAGGATATGTCCATCCAGGAGCTGGCGGTCTTGGT
ATCTGGGCAGAAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT